Recombinant Mouse C-terminal-binding protein 2 (Ctbp2)

Code CSB-YP006136MO
MSDS
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Source Yeast
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Code CSB-EP006136MO
MSDS
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Source E.coli
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Code CSB-EP006136MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006136MO
MSDS
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Source Baculovirus
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Code CSB-MP006136MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ctbp2
Uniprot No.
Alternative Names
Ctbp2C-terminal-binding protein 2; CtBP2
Species
Mus musculus (Mouse)
Expression Region
1-445
Target Protein Sequence
MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI LKDLATVAFC DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV IVRIGSGYDN VDIKAAGELG IAVCNIPSAA VEETADSTVC HILNLYRRNT WLYQALREGT RVQSVEQIRE VASGAARIRG ETLGLIGFGR TGQAVAVRAK AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS LHCNLNEHNH HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP ESLRNCVNKE FFVTSAPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP PAMEGIIPGG IPVTHNLPTV AHPSQAPSPN QPTKHGDNRE HPNEQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Corepressor targeting diverse transcription regulators. Isoform 2 probably acts as a scaffold for specialized synapses. Functions in brown adipose tissue (BAT) differentiation.
Gene References into Functions
  1. The authors postulate that RIBEYE disruption is partially compensated by several small active zone organization at each synaptic contact between inner hair cells and spiral ganglion neurons. PMID: 29328020
  2. studies revealed a new epigenetic pathway in the control of IL-2 production in systemic lupus erythematosus whereby low levels of miR-200a-3p accumulate the binding of the ZEB1-CtBP2 complex to the IL-2 promoter and suppress IL-2 production PMID: 28438897
  3. Our results show that RIBEYE is essential for synaptic ribbons as such, and may organize presynaptic nano-domains that position release-ready synaptic vesicles adjacent to Ca(2+) channels PMID: 26929012
  4. Ctbp2 preoccupies regions in active genes with the NuRD complex in undifferentiated ESCs that are directed toward H3K27me3 by PRC2 to induce stable silencing, which is pivotal for natural lineage commitment. PMID: 25944056
  5. Phosphorylation of KLF3 and CtBP2 by HIPK2 strengthens the interaction between these two factors and increases transcriptional repression by KLF3. PMID: 25659434
  6. propose that Zscan4 may regulate gene transcription in mouse ES cells through interaction with LSD1 and CtBP2 PMID: 24594919
  7. RIBEYE(B) interacts with ArfGAP3 in vivo and in vitro. PMID: 24719103
  8. CTBP2 is indispensable to create a chromatin environment conducive for RAR/RXR-mediated transcription by recruiting the histone acetyltransferase p300. PMID: 23775127
  9. Study demonstrates differential regional and subcellular expression patterns for the two CtBP family members in brain and reveals a previously unknown synaptic localization for CtBP2 in particular brain regions. PMID: 22745816
  10. Ctbp2 has a role in maintaining a balance in decisions to self-renewal and differentiate in embryonic stem cells and mesodermal progenitors PMID: 19825414
  11. The CtBP2 co-repressor function is regulated by an NADH-dependent effect on CtBP2 dimerization. PMID: 15037661
  12. calsenilin and CtBP2 are present in synaptic vesicles and can interact in vivo PMID: 16787403
  13. These results identify a new CtBP interaction motif and establish ZNF217 as a transcriptional repressor protein that functions, at least in part, by associating with CtBP. PMID: 16940172
  14. These results demonstrate that the PXDLS binding cleft is functionally important but suggest that it is primarily required for localization of the CtBP2 complex to promoter-bound transcription factors. PMID: 16940173
  15. analysis of the CtBP2 corepressor complex induced by E1A and modulation of E1A transcriptional activity by CtBP2 PMID: 17023432
  16. E1A may gain access to cellular promoters through conservved sequence-dependent interaction with CtBP2. PMID: 17546044
  17. cooperates with ZEB1 and HDAC1 to suppress IL-2 gene activation in T cells PMID: 19181930

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Subcellular Location
Nucleus. Cell junction, synapse.
Protein Families
D-isomer specific 2-hydroxyacid dehydrogenase family
Tissue Specificity
Found in all tissues except spleen and liver.
Database Links
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