Product Details

Purity

>85% (SDS-PAGE)

Target Names

Cd300lf

Uniprot No.

Q6SJQ7

Alternative Names

Cd300lf; Clm1; Lmir3; CMRF35-like molecule 1; CLM-1; CD300 antigen-like family member F; Leukocyte mono-Ig-like receptor 3; Myeloid-associated immunoglobulin-like receptor 5; MAIR-5; MAIR-V; CD antigen CD300f

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide which acts as a ligand. May act as a coreceptor for interleukin 4 (IL-4). Associates with and regulates IL-4 receptor alpha-mediated responses by augmenting IL-4- and IL-13-induced signaling. Negatively regulates the Toll-like receptor (TLR) signaling mediated by MYD88 and TRIF through activation of PTPN6/SHP-1 and PTPN11/SHP-2. Inhibits osteoclast formation. Induces macrophage cell death upon engagement.; (Microbial infection) Acts as a functional murine norovirus (MNV) receptor that mediates binding to the cell surface and is both necessary and sufficient for viral entry and replication. Primary determinant of MNV species tropism and is sufficient to render cells permissive to infection by MNV. Can render nonmurine mammalian cells susceptible to MNV infection.

Gene References into Functions

Docking of the cocomplex structures onto a cryo-EM-derived model of MNoV suggests that each virion can make multiple CD300lf engagements, and thus, infection may be driven by the avidity of cell surface clustered CD300lf. PMID: 30194229
sCD300lf bound on the mouse norovirus P domain. The receptor-binding site was located on the topside of the P2 subdomain and involved hydrophilic and hydrophobic interactions. The sCD300lf bound in a small cavity and was further supported by a positive surface charge on the sCD300lf and negative surface charge on the P domain. PMID: 29563286
CD300f helps to maintain immune homeostasis by promoting macrophage clearance of self-antigens, while conversely inhibiting DC uptake and presentation of self-antigens. PMID: 26768664
dextran sodium sulfate-treated Cd300f-/- mice exhibit attenuated disease activity and histopathology in comparison with wild type controls PMID: 27118491
data highlight a previously unappreciated role for dendritic cells in controlling gut homeostasis and show that CD300f-dependent regulation of apoptotic cell uptake is essential for suppressing overactive dendritic cell-mediated inflammatory responses, thereby controlling the development of chronic gut inflammation. PMID: 28414292
ceramide-CD300f binding inhibits LPS-induced skin inflammation, implicating CD300f as a negative regulator of Toll-like receptor 4 (TLR4) signaling in vivo PMID: 28073916
CD300lf is essential for murine noroviruse binding and replication in cell lines and primary cells. PMID: 27540007
CD300f is implicated in Wallerian degeneration and nerve regeneration by modulating both the influx and phenotype of macrophages PMID: 26259611
Y276 phosphorylation is necessary for enhanced CD300f-mediated phagocytosis through the recruitment of the p85alpha regulatory subunit of phosphatidylinositol-3-kinase PMID: 24477292
data highlight a previously unidentified role for CD300f in IL-4Ralpha-induced immune cell responses PMID: 26124135
CMRF35-like molecule 1 (CLM-1) regulates eosinophil homeostasis by suppressing cellular chemotaxis. PMID: 23820751
Several extracellular lipids (including ceramide) and lipoproteins were possible ligands for LMIR3. PMID: 23123064
We conclude that expression of CD300f conveys additional capacity to recognize phosphatidylserine to myeloid cells. PMID: 21865548
CLM-1 as a negative regulator of myeloid effector cells in autoimmune demyelination. PMID: 20038601
DIgR2 is a functionally inhibitory receptor and can mediate negative signaling to regulate DC-initiated antigen-specific T-cell responses. PMID: 16794255
These results indicate that CD300LF mediates caspase and endoplasmic reticulum stress-independent cell death by a novel mechanism. PMID: 18097021
LMIR3 has a unique property to associate with FcRgamma and thereby functions as an activating receptor in concert with TLR4 stimulation PMID: 19561101

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

CD300 family

Tissue Specificity

Expressed in myeloid cells. Present on the surface of macrophages (at protein level). Highly expressed by alveolar, splenic macrophages and bone marrow-derived dendritic cells. Expression is increased following aeroallergen challenge in macrophages, mast

Database Links

KEGG: mmu:246746

STRING: 10090.ENSMUSP00000102171

UniGene: Mm.277387

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Recombinant Mouse CMRF35-like molecule 1 (Cd300lf), partial

Product Details

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Target Background

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