Recombinant Mouse Carbonic anhydrase 15 (Ca15)

Code CSB-YP860403MO
MSDS
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Source Yeast
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Code CSB-EP860403MO
MSDS
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Source E.coli
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Code CSB-EP860403MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP860403MO
MSDS
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Source Baculovirus
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Code CSB-MP860403MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ca15
Uniprot No.
Alternative Names
Ca15; Car15Carbonic anhydrase 15; EC 4.2.1.1; Carbonate dehydratase XV; Carbonic anhydrase XV; CA-XV
Species
Mus musculus (Mouse)
Expression Region
19-324
Target Protein Sequence
VD SSGTWCYDSQ DPKCGPAHWK ELAPACGGPT QSPINIDLRL VQRDYTLKPF IFQGYDSAPQ DPWVLENDGH TVLLRVNSCQ QNCPAIRGAG LPSPEYRLLQ LHFHWGSPGH QGSEHSLDEK HGSMEMHMVH MNTKYQSMED ARSQPDGFAI LAVLLVEEDR DNTNFSAIVS GLKNLSSPGV AVNLTSTFAL ASLLPSALRL LRYYRYSGSL TTPGCEPAVL WTVFENTVPI GHAQVVQFQA VLQTGPPGLH PRPLTSNFRP QQPLGGRRIS ASPEASVRSS VSTLPCLHLA LVGLGVGLRL WQGP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Reversible hydration of carbon dioxide.
Gene References into Functions
  1. Car2, Car3, Car7, and Car15 mRNAs were barely within the detection limits in the mouse harderian gland. PMID: 20820888
  2. CA XV is expressed in the thick ascending limb of Henle and in the collecting ducts of mouse kidney and may have a role in the regulation of acid-base homeostasis PMID: 20224780
  3. CA XV is the first member of the alpha-CA gene family that is expressed in several species, but not in humans and chimpanzees. PMID: 16083424
  4. Mouse carbonic anhydrase XV, absent in humans, is a moderately active enzyme which may play a physiological role at least in the kidney. [review] PMID: 18336313
  5. cloning & purification of CA XV; the enzyme shows a moderate catalytic activity for the physiologic reaction, similarly to the physiologically relevant isoforms CA I, IV, VI, XII, and XIV, and it is susceptible to inhibition by sulfonamides and sulfamates PMID: 19193158

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Subcellular Location
Secreted.
Protein Families
Alpha-carbonic anhydrase family
Database Links

KEGG: mmu:80733

UniGene: Mm.390864

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