Recombinant Mouse Caspase-3(Casp3)

Code CSB-YP004548MO
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Source Yeast
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Code CSB-EP004548MO
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Source E.coli
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Code CSB-EP004548MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004548MO
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Source Baculovirus
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Code CSB-MP004548MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Casp3
Uniprot No. P70677
Alternative Names Casp3; Cpp32Caspase-3; CASP-3; EC 3.4.22.56; Apopain; Cysteine protease CPP32; CPP-32; LICE; Protein Yama; SREBP cleavage activity 1; SCA-1) [Cleaved into: Caspase-3 subunit p17; Caspase-3 subunit p12]
Species Mus musculus (Mouse)
Expression Region 29-175
Target Protein Sequence SG IYLDSSYKMD YPEMGICIII NNKNFHKSTG MSSRSGTDVD AANLRETFMG LKYQVRNKND LTREDILELM DSVSKEDHSK RSSFVCVILS HGDEGVIYGT NGPVELKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Triggers cell adhesion in sympathetic neurons through RET cleavage (By similarity). Cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes.
Gene References into Functions
  1. Pyrin signaling is dispensable for Clostridium difficile infection (CDI) associated intestinal epithelial cells death and for in vivo pathogenesis; C. difficile enterotoxins induce activation of executioner caspases 3/7 via the intrinsic apoptosis pathway indicating that caspase-3/7-mediated intestinal epithelial cells apoptosis is critical for in vivo host defense during early stages of CDI. PMID: 30451870
  2. Results show an association of PD with p53 and active caspase-3 overexpression and beta-adrenergic receptor underexpression in the heart, potentially promoting the cardiac autonomic dysfunction frequently observed in PD. PMID: 30135418
  3. Because DFNA5-induced secondary necrosis and GSDMD-induced pyroptosis are dependent on CASP3 activation, we propose that they are forms of programmed necrosis. PMID: 28045099
  4. Results revealed an interaction between Oocyte-G1 and Caspase-3. Expression levels of Caspase-3 were upregulated in cells overexpressing Oocyte-G1 and downregulated in Oocyte-G1 knockdown cells and suggest that casp3 is activated by oocyte-G1 promoting male germ cell apoptosis. PMID: 29802994
  5. NKG2D is upregulated, and ROS and caspase-3 are inhibited by Strongylocentrotus nudus egg polysaccharide-enhanced antitumor 5-FU in mice PMID: 27385218
  6. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy PMID: 28459430
  7. Caspases and their substrates are key mediators of apoptosis. PMID: 27552481
  8. SERT protects trophoblast cells against apoptotic cell death in caspase-3 independent pathway by terminating the 5-HT signaling. PMID: 28109119
  9. Prolonged treatment of human PMNs or mice bone marrow derived neutrophils (BMDN) with nitric oxide led to enhanced reactive oxygen species generation, caspase-8/caspase-3 cleavage, reduced mitochondrial membrane potential and finally cellular apoptosis. PMID: 27584786
  10. Induction of apoptosis in epithelial cancer cells was accompanied by a switch from glycolysis to oxidative phosphorylation, cytosol acidification and caspase-3 activation. PMID: 28063999
  11. This provides new insight into the immune phenotype, mechanisms, and signaling pathways that operate in microglial neurotoxic activation in amyotrophic lateral sclerosis. PMID: 28336525
  12. ULK1 is a novel substrate of Caspase-3 and upregulation of ULK1 drives autophagy initiation in leukemia cells. PMID: 28381396
  13. These results indicate that the caspase-3/p120 RasGAP stress-sensing module impacts on carcinogen-induced liver cancer incidence but not sufficiently so as to affect overall survival. PMID: 28150874
  14. report that mutating the caspase-3 consensus site in the EAAT2 sequence with an aspartate to asparagine mutation PMID: 28342750
  15. melatonin could inhibit TLR4 expression in hypoxic microglia followed by suppression of caspase3 activation leading to decrease in production of proinflammatory mediators PMID: 27812200
  16. A significant emergence of GFP-expressing PCs and interneurons in symptomatic, but not non-symptomatic, SCA1 mice 2 weeks after the MSC injection. PMID: 27802273
  17. Conversely, treatment with LY294002 (a selective inhibitor of Akt1) reversed the effects of quercetin. In conclusion, these findings highlight the important role of quercetin in protecting against cognitive deficits and inhibiting neuronal apoptosis via the Akt signaling pathway. We believe that quercetin might prove to be a useful therapeutic component in treating cerebral I/R diseases in the near future. PMID: 27450812
  18. cognitive decline was significantly correlated with increased levels of caspase activity and tau truncated by caspase-3 PMID: 27220334
  19. this study shows that Le Carbone, a charcoal supplement, attenuates experimental colitis through caspase 3 dependent apoptotic pathways PMID: 27978459
  20. Cepharanthine treatment reduced mitochondrial membrane potential and upregulated the level of cleaved caspases, including caspase-9 and caspase-3/7, in a dose-dependent fashion. PMID: 27121492
  21. Our results showed that Sca-1(+) MSCs inhibit the commitment of marginal zone B lymphocytes. The inhibition was exerted through lowered Caspase-3 expression. Furthermore, we found marginal zone B lymphocytes in spleen of Caspase-3 knockout mice decreased and Caspase-3 knockout Sca-1(+) MSCs accounted for the MZB lymphocytes decrease. PMID: 26861667
  22. caspase-3 is essential for a subset of social behaviors, but despite similar hyper-locomotion in both sexes, only male Casp3(-/-) mice exhibited social interaction deficits, which is interesting given the male bias of autism PMID: 26783106
  23. Study identified CASP-3 mRNA as a new miRNA-155 target in lipopolysaccharides-activated macrophages suggesting that through destabilization of CASP-3, miR-155 contributes to the protective role to sustain macrophage function in inflammation response. PMID: 26574931
  24. does not significantly contribute to cardiomyocyte death induced by transient coronary occlusion PMID: 26924441
  25. expression level of miR-98, which can regulate Caspase-3, was significantly decreased. Huwe1, the host gene of miR-98, was positively associated with miR-98 expression after Silica NP exposure PMID: 26263183
  26. administration of 0.3 IU/mL FSH during ovarian cryopreservation by vitrification can maintain ovarian survival during ovarian vitrification and increases the blood supply with avascular transplantation via upregulation of Cx43, Cx37, and VEGF/VEGFR2 PMID: 26539488
  27. that caspase-3-mediated nuclear opening formation accompanied by histone release from the opening is a critical step toward chromatin condensation during erythropoiesis in mice PMID: 26954545
  28. MicroRNA-124 and microRNA-137 cooperatively control caspase-3 activity through BCL2L13 in hippocampal neural stem cells. PMID: 26207921
  29. impaired mTORC1-Homer-3 activity underlies PC susceptibility in Spinocerebellar ataxia type 1 and presents a promising therapeutic target PMID: 26748090
  30. GSAP cleavage via caspase-3 is regulated and depend upon the availability of 5-Lipoxygenase in Alzheimer's disease. PMID: 26076991
  31. The study demonstrates sublethal caspase-activation has an important role in cardiomyocyte differentiation and may have significant implications for promoting cardiac regeneration after myocardial injury involving exogenous or endogenous cell sources. PMID: 25763592
  32. Parkinson disease induction increased the expression of the apoptotic mediators p53 and active caspase-3 in gastrocnemius muscle. PMID: 25704481
  33. The results reveal that executioner caspases can modulate heart's cellularity and maturation during development, contributing novel information about caspase biology and heart development. PMID: 26121671
  34. Caspase-3 activity was significantly elevated after I/R in both postischemic groups PMID: 26009812
  35. Uterine endoplasmic reticulum stress-unfolded protein response regulation of gestational length is caspase-3 and -7-dependent. PMID: 26504199
  36. a novel cell fate determination mechanism to ensure cells undergo programed cell death through interfering with RPS3/NF-kappaB-conferred anti-apoptotic transcription by the fragment from partial p65 cleavage by activated Caspase-3 PMID: 26526615
  37. results indicate a novel role of Casp-2 in the osteoclast as a modulator of total and mitochondrial ROS and osteoclast differentiation PMID: 25796569
  38. The results of this study showed that the Casp3 gene is important for the proper function of the semicircular canals but less important for the otolith organ function. PMID: 25827332
  39. Preclinical studies identify non-apoptotic low-level caspase-3 as therapeutic target in pemphigus vulgaris. PMID: 25748204
  40. Caspase 3 cleavage of Pax7 has a role in inhibiting self-renewal of satellite cells PMID: 26372956
  41. deletion of p21Cip1/Waf1 and caspase-3 accelerates proliferation and partially rescues the differentiation defects of caspase-3 deficient hematopoietic stem cells PMID: 25286245
  42. The hepatic expression level of cleaved caspase-3 protein in the group treated concomitantly with doxorubicin and theanine was significantly lower than that in the doxorubicin treated group. PMID: 25680506
  43. The present results indicate that a regular Met supply lessens the biochemical changes, damage, and caspase-dependent apoptosis provoked by recurrent dietary amino acid deprivation in the mouse liver PMID: 25575574
  44. Interleukin-1beta enhances FasL-induced caspase-3/-7 activity without increasing apoptosis in primary mouse hepatocytes PMID: 25551609
  45. Induction of cIAP2 expression upon microglia activation prevents the conversion of caspase-3 p19 subunit to p17 subunit. PMID: 25501826
  46. Taken together, our results demonstrate that amifostine increases FAS and caspase-3 expression in colonic tissue of irradiated mice. PMID: 25964561
  47. miR-21 is endowed with anti-apoptotic properties by suppressing the expression of PDCD4 gene and active caspase 3/8 fragments in the condition of renal IRI PMID: 25322693
  48. Results suggest that miR-378 overexpression attenuates high glucose-suppressed osteogenic differentiation through targeting CASP3 and activating the PI3K/Akt pathway. PMID: 25400823
  49. Results show that human or mouse cells exposed to ionizing radiation or other stresses can survive with persistent caspase-3 activation, which in turn promotes genetic instability and oncogenic transformation. PMID: 25866249
  50. cancer stimulates p-Stat3 in muscle, activating protein loss by stimulating caspase-3, myostatin, and the ubiquitin-proteasome system. PMID: 25787076
  51. Data indicate that procaspase-3 has a non-apoptotic function; it regulates the secretion of fibronectin and influences morphology, adhesion and migration. PMID: 24610949
  52. Among 46 putative substrates identified in MN9D neuronal cells, we further evaluated whether caspase-3-mediated cleavage of anamorsin, a recently recognized cell death-defying factor in hematopoiesis, is a general feature of apoptosis. PMID: 24973211
  53. Effect of cortisol on caspases in the co-cultured C2C12 and 3 T3-L1 cells. PMID: 24752940
  54. Proteinase 3-mediated caspase-3 activation controls neutrophil spontaneous death PMID: 25180606
  55. activation of caspase-3 may play an inhibitory role at the late stage of RANKL-induced osteoclast differentiation. PMID: 24523219
  56. these data suggest that the metabolic state conferred by IL-15 inhibits T-cell apoptosis in part by maintaining low levels of active caspase-3 via S-nitrosylation. PMID: 24510126
  57. Data indicate that accumulation of [(18)F]FB-VAD-FMK agreed with elevated caspase-3 activity in human xenograft colorectal and colon cancer tissues. PMID: 24573549
  58. Dual vulnerability of TDP-43 to calpain and caspase-3 proteolysis after neurotoxic conditions and traumatic brain injury. PMID: 24917042
  59. This study supports a role for caspase 3 cleaved IP3 R1 and C terminal-IP3 R1 in regulating Ca(2+) homeostasis and the endoplasmic reticulum Ca(2+) content during oocyte maturation. PMID: 24692207
  60. our data identify a previously unknown caspase-3-dependent mechanism that constrains stroke-induced endogenous neurogenesis and should revitalize interest in targeting caspase-3 for treatment of stroke PMID: 23939807
  61. An unexpected function of C3a/C3aR signaling during host immune response suppresses Fas expression and caspase-3 activity while increasing Bcl-2 expression, providing protection to myeloid and lymphoid cells against L. monocytogenes-induced apoptosis. PMID: 24981453
  62. these findings support the existence of a microRNA-21-responsive PDCD4/caspase-3 pathway in the pulmonary tissues that when active serves to promote endothelial apoptosis in vitro and pulmonary hypertension in vivo. PMID: 24732886
  63. OCT-P407 induces mRNA expression of SSTR-2 and caspase-3 and decreases that of VEGF in mice. PMID: 24173662
  64. findings demonstrate that focal activation of caspase-9 and -3 at neuromuscular junctions is essentially responsible for subsynaptic DNA damage and apoptotic synaptic degeneration in slow-channel myasthenic syndrome PMID: 23943790
  65. These findings support a prominent role for hepatocyte caspase 3 activation in NASH-related apoptosis, fibrogenesis and fibrosis which in part is mediated via CCR2-dependent infiltration of Ly6c positive monocytes. PMID: 24795036
  66. Kainic acid induced neurodegeneration and aroused several caspase 3-mediated molecular and cellular changes such as dendritic plasticity, neurogenesis, and gliosis. Acute caspase 3 activation occurred in pyramidal neurons as well as in hilar interneurons. PMID: 24313976
  67. Which was significantly attenuated in the presence of the caspase-3 inhibitor. PMID: 24362325
  68. these results support a causative role of VEGF in perinatal cerebral hemorrhage and implicate its downstream proteases as potential therapeutic targets. PMID: 23843451
  69. lysosomal membrane permeability induced by TNFalpha plus cycloheximide, the release of lysosomal cathepsins and reactive oxygen species formation require the caspase-3-mediated cleavage of the p75 NDUFS1 subunit of respiratory complex I. PMID: 23788428
  70. HuR undergoes cleavage modification by caspase-3 following IR-induced oral mucositis and subsequently promotes the expression of the pro-apoptotic factor BAX (Bcl-2-associated X protein), as well as cell death. PMID: 24362034
  71. Caspase-3 knock-out mice have increased spine density and altered miniature EPSCs, confirming a physiological involvement of caspase-3 in the regulation of spines in vivo. PMID: 24478350
  72. The activity of caspase-3, -8 and -9 was significantly increased in the isoflurane-treated cells compared with the untreated cells. PMID: 23922164
  73. The finding that BMMCs secrete caspase-3 and gzmB after Ag stimulation suggests that both proteases contribute to extracellular MC-mediated proteolytic events. PMID: 24414824
  74. After activation by caspase-9, caspase-3 inhibits ROS production and is required for efficient execution of apoptosis, while effector caspase-7 is required for apoptotic cell detachment. PMID: 23834359
  75. the presence of active caspase-3-like activity in granule-like compartments close to the plasma membrane was demonstrated. Moreover, it was shown that mast cell activation caused release of the caspase-3 to the cell exterior. PMID: 23817418
  76. Alpha B-crystallin inhibits caspase 3 activation in cells primed for apoptosis by retinoblastoma protein inactivation. PMID: 23471649
  77. Nek5 promotes myogenic differentiation through up-regulation of caspase-3 activity. PMID: 23727203
  78. Caspase 3 cleavage of papillomavirus E1 protein, which has been shown to be necessary for vegetative viral DNA replication, restores the DNA binding activity to phosphorylated E1. PMID: 23637413
  79. Caspase-3 expression in the tissues of experimental melanoma and its metastases during matrix metalloproteinase-9 inhibition PMID: 23805468
  80. High expression of TNF-alpha and Casp3 might be associated with monocyte apoptosis during alveolar Echinococcus infection. PMID: 23072136
  81. Myocardial calpain induces myocardial caspase-3 activation and apoptosis in septic mice via the activation of the Hsp90/Akt pathway. PMID: 23425388
  82. Caspase-3 plays a key role in disuse muscle atrophy and associated decreased tension, presumably by acting on the apoptosis and inflammation pathways. PMID: 23401051
  83. Survivin prevents apoptosis by binding to caspase-3 in astrocytes infected with the BeAn strain of Theiler's murine encephalomyelitis virus. PMID: 22638909
  84. Data suggest that control of CYP24A1 increase during diabetes has a beneficial effect on senescence induction and caspase-3 increased expression. PMID: 23119081
  85. both Caspase-3 and Caspase-6 are implicated in axon degeneration that occurs as a part of normal development. PMID: 23223278
  86. putple rice extract (PRE), cyanidin, and HHPG suppress tunicamycin-induced retinal ganglion cell death at least partly by inhibiting activation of caspase-3; PRE and its main constituents prevent retinal disease caused by ER stress PMID: 23123597
  87. exposure to the EMF of 1950-MHz TD-SCDMA may not promote the tumor formation, but continuous exposure damaged the mitochondria of astrocytes and induce apoptosis through a caspase-3-dependent pathway with the involvement of bax and bcl-2. PMID: 22870319
  88. Caspase-3 siRNA may reduce neurotoxicity induced by aluminum PMID: 21972528
  89. TNF-alpha-induced apoptosis and Caspase-3 expression in mouse skin keratinocytes in vivo and in vitro PMID: 22585037
  90. Caspase-3 is a stress intensity sensor that controls cell fate by either initiating a RasGAP cleavage-dependent cell resistance program or a cell suicide response mediated by akt. PMID: 22949508
  91. Levels of caspase-3 activity, differ among alphabeta and gammadelta effector T-cell subsets and the degree of redistribution of active caspase from the cell membrane to the cytoplasm upon TCR restimulation is proportional to the susceptibility to cell death. PMID: 22117649
  92. Activation of Toll-like receptor (TLR)7, a receptor sensing single-stranded viral RNA, causes neuronal cell death through caspase-3 and the adapter molecule MyD88 in a cell-autonomous fashion. PMID: 22745379
  93. Hepatitis C virus infection induces death of pancreatic beta cells through an endoplasmic reticulum stress-involved, caspase 3-dependent, special pathway. PMID: 22675572
  94. ER stress contributes, at least in part, to hepatic SREBP-1c activation and lipid accumulation in fructose-evoked nonalcoholic fatty liver disease. PMID: 22698815
  95. Modification of antiapoptotic signaling partners allows the pregnant uterus to escape the apoptotic action of elevated active caspase 3 levels but also functions to limit the levels of active uterine caspase 3 near term. PMID: 21566000
  96. Vestibular phenotype and inner ear anatomy are characterized in a caspase-3 deficient mouse strain. PMID: 21988729
  97. Cisplatin increases expression of Casp3 which can cause apoptosis of spiral ganglion cells. PMID: 21038681
  98. In microglial cells IL-4 increases the levels of cleaved caspase 3 and poly ADP-ribose polymerase (PARP), which is down-stream of activated caspase 3. PMID: 21624466
  99. The expression levels of PDCD5 and caspase 3 in the cochlea of C57 mice increase with age. PMID: 22177044
  100. Gsdma3 gene mutation interfered with catagen formation during mouse hair follicle cycle and, by upregulation of Caspase-3 expression and promotion of apoptosis, Gsdma3 gene could play an essential role in normal catagen induction. PMID: 21805153

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Subcellular Location Cytoplasm
Protein Families Peptidase C14A family
Tissue Specificity Highest expression in spleen, lung, liver, kidney and heart. Lower expression in brain, skeletal muscle and testis.
Database Links

KEGG: mmu:12367

STRING: 10090.ENSMUSP00000091238

UniGene: Mm.34405

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