Product Details

Purity

>85% (SDS-PAGE)

Target Names

Casp4

Uniprot No.

P70343

Alternative Names

CASP-11; CASP-4; CASP11; CASP4; CASP4_MOUSE; Caspase 11; Caspase 11 apoptosis related cysteine peptidase; Caspase 11 apoptosis related cysteine protease; Caspase 4; Caspase 4 apoptosis related cysteine peptidase; Caspase-11; Caspase-4 subunit p20; CASPL; Ich 3; Ich3; ICH3 protease; Protease ICH-3

Species

Mus musculus (Mouse)

Expression Region

81-266

Target Protein Sequence

PGSHHGEANL EMEEPEESLN TLKLCSPEEF TRLCREKTQE IYPIKEANGR TRKALIICNT EFKHLSLRYG ANFDIIGMKG LLEDLGYDVV VKEELTAEGM ESEMKDFAAL SEHQTSDSTF LVLMSHGTLH GICGTMHSEK TPDVLQYDTI YQIFNNCHCP GLRDKPKVII VQACRGGNSG EMWIRE

Protein Length

partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Inflammatory caspase that acts as an essential effector of NLRP3 inflammasome-dependent CASP1 activation and IL1B and IL18 secretion in response to non-canonical activators, such as UVB radiation, cholera enterotoxin subunit B and cytosolic LPS, as well as infection with Gram-negative bacteria. Thiol protease that cleaves a tetrapeptide after an Asp residue at position P1. Independently of NLRP3 inflammasome and CASP1, promotes pyroptosis, through GSDMD cleavage and activation, followed by IL1A, IL18 and HMGB1 release in response to non-canonical inflammasome activators. Plays a crucial role in the restriction of Salmonella typhimurium replication in colonic epithelial cells during infection: in later stages of the infection, LPS from cytosolic Salmonella triggers CASP4 activation, which catalyzes cleavage of GSDMD, resulting in pyroptosis of infected cells and their extrusion into the gut lumen, as well as in IL18 secretion. Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. Involved in LPS-induced IL6 secretion; this activity may not require caspase enzymatic activity. Involved in cell death induced by endoplasmic reticulum stress. Activated by direct binding to LPS without the need of an upstream sensor. Does not directly process IL1B. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation.

Gene References into Functions

Together, these data suggest that caspase-11/IL-1alpha pathway plays an important role in defending against Klebsiella pneumoniae by recruiting neutrophils in the early stage of infection. PMID: 28939441
Results indicate functional differences between human caspase-4 and murine caspase-11 (caspase 4). PMID: 29339744
Data suggest that Brucella melitensis effector protein TcpB suppresses caspase-4/11--mediated inflammation, subverting inflammasome activation in mouse macrophages; TcpB induces ubiquitination/degradation of caspase-4/11; TcpB suppresses caspase-4/11--mediated pyroptosis and proinflammatory responses induced by lipopolysaccharide. PMID: 29061850
systemic exposure to the bacterial endotoxin lipopolysaccharide (LPS) causes severe endothelial pyroptosis that is mediated by the inflammatory caspases, human caspases 4/5 in human ECs, or the murine homolog caspase-11 in mice in vivo. PMID: 28990935
Caspase-l, Caspase-11 and Dectin-1/syk pathway are involved in the IL-1beta generation in fungal keratosis. PMID: 28167474
These data indicate that the caspase-11-mediated innate immune response plays a crucial role in defending against Acinetobacter baumannii. PMID: 28760936
Hyperactive dendritic cells induce potent adaptive immune responses and are elicited by caspase-11, an enzyme that binds oxidized phospholipids and bacterial lipopolysaccharide. PMID: 27103670
gasdermin D is essential for caspase-11-dependent pyroptosis and interleukin-1beta maturation PMID: 26375259
But not in Casp11(-/-), Panx1(-/-), or P2x7(-/-) mice. PMID: 26572062
active caspase-11 leads to a drop of intracellular potassium levels, which is necessary to activate NLRP3. PMID: 26173909
These results suggest a role for other caspase-11 effector mechanisms such as pyroptosis in protection against intestinal inflammation. PMID: 24850431
Caspase-11 limits the response of CD8+ T-cells to low-abundance and low-affinity antigens. PMID: 25980012
These data show that both Candida albicans Sap2 and Sap6 can activate caspase-11 through type I interferon (IFN) production. PMID: 25712931
these data suggest that IFN-gamma-mediated caspase-11 expression has a key role maintaining intestinal epithelial barrier integrity in vivo during experimentally induced acute colitis. PMID: 25548224
The caspase-4/11 inflammasome also governs activation of the proinflammatory cytokine, interleukin (IL)-18, in response to intracellular and extracellular enteric pathogens. PMID: 25121752
Lipopolysaccharides may be sensed in the cytosol of cells by a noncanonical inflammasome pathway, resulting in the activation of the cysteine protease caspase-11. [Review] PMID: 25347553
identify caspase-11 as a critical factor protecting the host during acute dextran sulfate sodium-induced colonic injury and inflammation but not during chronic inflammation and tumorigenesis PMID: 25414099
function of caspase-4/5/11 represents a new mode of pattern recognition in immunity and also an unprecedented means of caspase activation PMID: 25119034
caspase-4 can support activation of caspase-1 and secretion of IL-1beta and IL-18 in response to priming signals (LPS or Pam3CSK4) alon PMID: 24879791
a cluster of small interferon-inducible GTPases, the so-called guanylate-binding proteins, is required for the full activity of the non-canonical caspase-11 inflammasome during infections with vacuolar Gram-negative bacteria PMID: 24739961
IFN-inducible guanylate binding protein (Gbp) proteins stimulate caspase-11-dependent, cell-autonomous immunity in response to cytoplasmic lipopolysaccharides. PMID: 24715728
Emerging data point to a cascade of events during which caspase-11 promotes and corroborates caspase-1 activity through NLRP3 inflammasomes. Caspase-11 is also capable of independently inducing pyroptotic cell death.[Review] PMID: 23870309
virulent, but not avirulent, bacteria trigger a rapid caspase-11-dependent innate immune response important for host defense. PMID: 23762026
review gathers together studies on both the mechanisms and implications of caspase-11-mediated noncanonical inflammasome activation, and discuss the emerging importance of this pathway in regulating host defense against intracellular bacterial pathogens PMID: 24037676
study reports that caspase-11 is required for innate immunity to cytosolic, but not vacuolar, bacteria PMID: 23348507
TLR4- and TRIF-induced caspase-11 synthesis is critical for noncanonical Nlrp3 inflammasome activation in macrophages infected with enteric pathogens. PMID: 22898816
non-canonical caspase-11 activation contributes to macrophage death during S. typhimurium infection PMID: 22895188
Caspase-11 activation via the TLR4-TRIF-IFNbeta pathway synergizes with the NLRP3 pathway to coordinate caspase-1-dependent IL-1beta and IL-18 secretion and also leads to caspase-1-independent cell death. PMID: 22819539
found that, despite extensive backcrossing into a C57BL/6 background, c-IAP1(-/-) animals are also deficient in caspase 11 PMID: 22332634
data highlight a unique pro-inflammatory role for caspase-11 in the innate immune response to clinically significant bacterial infections PMID: 22002608
PARP-1, as a transcriptional cofactor for NF-kappaB, regulates the induction of caspase-11 at a transcriptional level. PMID: 21530491
TNFA deletion alters apoptosis as well as caspase 3 and 4 expression during otitis media. PMID: 21269505
11 is an essential molecule in NO-independent apoptotic pathway of activated astrocytes. PMID: 11902113
gene expression in response to LPS and interferon-gamma requires NF-kappaB and Stat1 binding PMID: 12198138
In a mouse model of ALS neurodegeneration, inflammatory responses, and disease onset and progression in SOD1 G93A transgenic mice were not altered by the ablation of caspase-11 gene PMID: 12843244
the neurotoxic effects of LPS on nigral dopaminergic neurons are mediated by microglial activation, interleukin-1beta, and caspase-11 expression in mice PMID: 15383538
Caspase-11 regulates cell migration during inflammation. PMID: 17293856
Our results suggest that neuroinflammation related to the caspase-11 cascade in the striatum regulates differentiation of neural stem cells in the SVZ of our mouse model of PD. PMID: 17963913
Caspase-11 plays a critical role in TPEN-induced apoptosis. PMID: 18474237
Interferon beta induces mature dendritic cell apoptosis through caspase-11/caspase-3 activation. PMID: 19531658
Ask1 regulates the expression of caspase-11 following LPS stimulation PMID: 19695249

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Subcellular Location

Cytoplasm, cytosol. Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. Mitochondrion. Inflammasome. Secreted.

Protein Families

Peptidase C14A family

Tissue Specificity

Widely expressed, including in thymus, lung and spleen (at protein level). Very low levels, if any, in the brain.

Database Links

KEGG: mmu:12363

STRING: 10090.ENSMUSP00000027012

UniGene: Mm.1569

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Code	CSB-YP004549MO
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP004549MO
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP004549MO-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP004549MO
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP004549MO
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Recombinant Mouse Caspase-4 (Casp4), partial

Product Details

Customer Reviews and Q&A

Target Background

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