Recombinant Mouse Ceramide kinase (Cerk)

Code CSB-YP005256MO
MSDS
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Source Yeast
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Code CSB-EP005256MO
MSDS
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Source E.coli
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Code CSB-EP005256MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005256MO
MSDS
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Source Baculovirus
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Code CSB-MP005256MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cerk
Uniprot No.
Alternative Names
Cerk; Ceramide kinase; mCERK; EC 2.7.1.138; Acylsphingosine kinase
Species
Mus musculus (Mouse)
Expression Region
1-531
Target Protein Sequence
MGAMGAAEPL HSVLWVKRRR CAVSLEPARA LLRWWRSPEP GPSAPGADAR SVLVSEIIAV EEKDDCEKHA SSGRWHKMEN PFAFTVHRVK RVRHHRWKWA RVTFWSADEQ LCHLWLQTLR GLLESLTSRP KHLLVFINPF GGKGQGKRIY EKTVAPLFTL ASITTEIIIT EHANQAKETL YEINTDSYDG IVCVGGDGMF SEVLHGVIGR TQQSAGIDPN HPRAVLVPST LRIGIIPAGS TDCVCYSTVG TNDAETSALH IIIGDSLAID VSSVHYHNTL LRYSVSLLGY GFYGDLIKDS EKKRWMGLVR YDFSGLKTFL SHQYYEGTLS FLPAQHTVGS PRDNKPCRAG CFVCRQSKQQ LEEEEKKALY GLENAEEMEE WQVTCGKFLA INATNMSCAC PRSPGGLSPF AHLGDGSSDL ILIRKCSRFN FLRFLIRHTN QEDQFDFTFV EVYRVKKFHF TSKHVEDEDN DSKEQEKQKF GKICKDRPSC TCSASRSSWN CDGEVMHSPA IEVRVHCQLV RLFARGIEEE S
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Shows a greater preference for D-erythro isomer of ceramides. Binds phosphoinositides.
Gene References into Functions
  1. The depletion of CerK enhances the pathology of colitis and lethal responses in DSS-treated mice. PMID: 29709917
  2. these studies demonstrate a novel requirement for the CERK-derived C1P in the proper healing response of wounds. PMID: 24823941
  3. there are significant differences in eicosanoid levels in ex vivo CERK(-/-) cells compared with wild-type counterparts, but the effect of the genetic ablation of CERK on eicosanoid synthesis and the serum levels of C1P was not apparent in vivo PMID: 23576683
  4. role of ceramide kinase in obesity and insulin resistance PMID: 22465662
  5. Results indicate that C1P produced by CERK has a negative effect on the processing/secretion of TNFalpha via modulation of TACE activity. PMID: 22009748
  6. role of CerK on the activation of mast cells PMID: 20678580
  7. The mechanism of ceramide-mediated inhibition is likely to involve conversion of ceramide to ceramide 1-phosphate by CERK. PMID: 21041721
  8. LPS inhibits the transcriptional activity of the Cerk proximal promoter;transcriptional repression of Cerk by LPS is not a primary consequence of LPS-induced cell cycle blockade. PMID: 20849823
  9. Findings suggest that CerK is not necessary for survival at an individual level, but might be involved in higher brain function related to emotion. PMID: 17888878
  10. CerK is essential to ceramide 1-phosphate formation via phosphorylation of Cer PMID: 18086664
  11. Ceramide kinase is a key regulator of ceramide 1-phosphate, dihydroceramide and ceramide levels, with important implications for neutrophil homeostasis and innate immunity regulation. PMID: 18292572

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein.
Tissue Specificity
High level expression in heart, brain, testis and pancreas; low expression in spleen, liver and lung; not detected in skeletal muscle.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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