Recombinant Mouse Chitotriosidase-1 (Chit1)

Code CSB-YP861564MO
MSDS
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Source Yeast
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Code CSB-EP861564MO
MSDS
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Source E.coli
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Code CSB-EP861564MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861564MO
MSDS
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Source Baculovirus
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Code CSB-MP861564MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Chit1
Uniprot No.
Alternative Names
Chit1Chitotriosidase-1; EC 3.2.1.14; Chitinase-1
Species
Mus musculus (Mouse)
Expression Region
22-464
Target Protein Sequence
AKLVCYLTN WSQYRTEAVR FFPRDVDPNL CTHVIFAFAG MDNHQLSTVE HNDELLYQEL NSLKTKNPKL KTLLAVGGWT FGTQKFTDMV ATASNRQTFV KSALSFLRTQ GFDGLDLDWE FPGGRGSPTV DKERFTALIQ DLAKAFQEEA QSSGKERLLL TAAVPSDRGL VDAGYEVDKI AQSLDFINLM AYDFHSSLEK TTGHNSPLYK RQGESGAAAE QNVDAAVTLW LQKGTPASKL ILGMPTYGRS FTLASSSDNG VGAPATGPGA PGPYTKDKGV LAYYEACSWK ERHRIEDQKV PYAFQDNQWV SFDDVESFKA KAAYLKQKGL GGAMVWVLDL DDFKGSFCNQ GPYPLIRTLR QELNLPSETP RSPEQIIPEP RPSSMPEQGP SPGLDNFCQG KADGVYPNPG DESTYYNCGG GRLFQQSCPP GLVFRASCKC CTWS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Degrades chitin, chitotriose and chitobiose. May participate in the defense against nematodes and other pathogens.
Gene References into Functions
  1. No significant differences were observed between experimental autoimmune encephalitis and control groups for CHIT-1 expression. PMID: 26079949
  2. CHIT1 exerts protective effects against atherosclerosis by suppressing inflammatory responses and polarizing macrophages toward an M2 phenotype, and promoting lipid uptake and cholesterol efflux in macrophages. PMID: 23685110
Subcellular Location
Secreted. Lysosome.
Protein Families
Glycosyl hydrolase 18 family, Chitinase class II subfamily
Tissue Specificity
Highly expressed in tongue, stomach, kidney, brain, skin, testis, and bone marrow. Low level of expression was found in lung, heart, spleen, small intestine, and liver. Not detectable in pancreas, salivary gland, large intestine, uterus, or peripheral blo
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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