Recombinant Mouse Chorion-specific transcription factor GCMa(Gcm1)

Code CSB-YP009323MO
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Source Yeast
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Code CSB-EP009323MO
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Source E.coli
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Code CSB-EP009323MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009323MO
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Source Baculovirus
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Code CSB-MP009323MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Gcm1
Uniprot No. P70348
Alternative Names Gcm1; GcmaChorion-specific transcription factor GCMa; GCM motif protein 1; mGCM1; mGCMa; Glial cells missing homolog 1
Species Mus musculus (Mouse)
Expression Region 1-436
Target Protein Sequence MELDDFDPED KEILSWDIND VKLPQNVKTT DWFQEWPDSY VKHIYSSDDR NAQRHLSSWA MRNTNNHNSR ILKKSCLGVV VCSRDCSTEE GRKIYLRPAI CDKARQKQQR KSCPNCNGPL KLIPCRGHGG FPVTNFWRHD GRFIFFQSKG EHDHPRPETK LEAEARRAMK KVHMASASNS LRMKGRPAAK ALPAEIPSQG SLPLTWSFQE GVQLPGTYST PLIANAPQQN SLNDCLSFPK SYDLGGSTEL EDPTSTLDSM KFYERCKFSS SRIYGSEEQF QPPVPGTYGD YEDLQTWNKN VALGRNPSDD IYYPAYPLPV ASWPYDYFPS QNSLEHLPQQ VPSEPPAAQP GCHPLWSNPG GEPYEEKVSV DLSSYVPSLT YHPPQQDPFL LTYGSPTQQQ HALPGKSNRW DFDEEMACMG LDHFNNEMLL NFCSLR
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Transcription factor that is necessary for placental development
Gene References into Functions
  1. GCM1-directed villous trophoblast differentiation is repressed by DREAM PMID: 23300953
  2. Data show that the GCM1/syncytin-B pathway is significantly downregulated in the placenta of BCL9L-deficient mice and that the fusion and differentiation of ST-II cells are blocked. PMID: 22109522
  3. as in mice, fetoplacental vascularity increased when GCM1 protein expression decreased (R(2)=-0.45; P<0.05). PMID: 22275534
  4. Gcm1 (glial cells missing 1) are involved in the epigenetic regulation of Hes5 transcription by DNA demethylation independently of DNA replication. PMID: 21765423
  5. studies identify a novel function of p45NF-E2 during placental development: in trophoblast cells, p45NF-E2 represses Gcm1 and syncytiotrophoblast formation via acetylation PMID: 21558372
  6. Gcm1 expression defines three stages of chorio-allantoic interaction during placental development PMID: 12049764
  7. Brief ectopic expression of Gcm1 in mouse embryonic tail bud induces failure of the neural tube to close (spina bifida) and multiple neural tubes (diastematomyelia). PMID: 12135932
  8. GCM domain as a new type of Zn-coordinating, sequence-specific DNA-binding domain. PMID: 12297286
  9. mGCMa expression is primarily postnatal, arguing for a role in organ function rather than organ development PMID: 12351183
  10. the shaping of the maternal blood spaces within the labyrinth was dependent on chorioallantoic morphogenesis and therefore disrupted in Gcm1 mutants PMID: 12376109
  11. Data describe the crystal structure of the glial cell missing (GCM) domain from murine GCMa bound to its octameric DNA target site at 2.85 A resolution. PMID: 12682016
  12. Regulation of subcellular localization has important implications for GCMa/Gcm-1 function. PMID: 14572643
  13. Gcm1 promotes cell cycle exit and restriction towards the syncytiotrophoblast cell fate. PMID: 15196947
  14. Pitx proteins influence GCMa-dependent promoter activation in a cell-specific manner PMID: 15385555
  15. Identification of integrin-alpha4, Rb1, and syncytin a as murine placental target genes of the transcription factor GCMa/Gcm1 PMID: 18167345
  16. Gcm1 is co-expressed with Cebpa and Synb in basal cells in contact with the allantois. PMID: 18448564

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Subcellular Location Nucleus
Tissue Specificity Placenta specific.
Database Links

KEGG: mmu:14531

STRING: 10090.ENSMUSP00000024104

UniGene: Mm.1400

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