Recombinant Mouse Crumbs homolog 1 (Crb1), partial

Code CSB-YP855357MO
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Source Yeast
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Code CSB-EP855357MO
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Source E.coli
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Code CSB-EP855357MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP855357MO
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Source Baculovirus
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Code CSB-MP855357MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Crb1
Uniprot No.
Alternative Names
Crb1; Protein crumbs homolog 1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a role in photoreceptor morphogenesis in the retina. May maintain cell polarization and adhesion.; May play a role in epidermal tissue morphogenesis. May function in cell attachment for stratified epithelial organization.
Gene References into Functions
  1. This study showed that The geographic distribution of subretinal microglia/macrophages changes with age in both Crb1 rd8/rd8 and C57BL mice, but the total number of microglia only increases in Crb1 rd8/rd8 mice,and pro-inflamatory phenotype. PMID: 25588310
  2. The retinal phenotype of Grk1-/- mice is compromised by a Crb1 rd8 mutation. PMID: 26664249
  3. Presence of rd8 (Crb1) mutation does not alter the ocular phenotype of late-onset retinal degeneration mouse model carrying the Ctrp5 mutation. PMID: 25814825
  4. Results show that Crb1rd8/rd8 mutation is necessary, but not sufficient for the development of all the degenerative features. PMID: 25147295
  5. Study showed that CRB1 and CRB2 in human retinas have an opposite pattern of expression in Muller glia and photoreceptor cells compared with mouse retinas, and that Crb2 influences the severity of the murine Crb1-linked retinal dystrophies. PMID: 24565864
  6. These findings suggest that CRB1 and CRB2 suppress late progenitor pool expansion by regulating multiple proliferative signaling pathways. PMID: 24339791
  7. The C57BL/6NJ-Crb1(rd8) mutation and its associated retinal phenotypes were corrected efficiently by TALEN-mediated homology-directed repair. PMID: 24346171
  8. This study demonistrated that the Rd8 mutation in the Crb1 gene of CD11c-eYFP transgenic reporter mice results in abnormal numbers of CD11c-positive cells in the retina. PMID: 23860032
  9. Data show the deletion of Pals1 leads to the disruption of the apical localization of Crb polarity complex proteins Crb1, Crb2 and Crb3 in retinal progenitors and the adult retina. PMID: 22398208
  10. CRB1 mutations lead to early-onset severe loss of vision with thickened, disorganized, nonseeing retina. Impaired peripheral vision can persist in late disease stages. PMID: 21757580
  11. Isolation of Crb1, a mouse homologue of Drosophila crumbs, and analysis of its expression pattern in eye and brain. PMID: 11744384
  12. During light exposure Crb1 is essential to maintain, but not assemble, adherens junctions between photoreceptors and Muller glia cells and prevents retinal disorganization and dystrophy. PMID: 15316081
  13. RNA interference-induced silencing of the Crb1-interacting-protein Pals1 (protein associated with Lin7; Mpp5) in Muller glial cells resulted in loss of Crb1, Crb2, Mupp1 and Veli3 protein localization and partial loss of Crb3. PMID: 16885194
  14. The extracellular domain of Crb1 positively regulates pituitary tumor transforming gene 1 (Pttg1) transcript levels. PMID: 17234588
  15. We propose that Crb1 is required to regulate number and size of these Muller glia cell villi. The subsequent loss of retinal integrity resulted in neovascularization, in which blood vessels of the choroid protruded into the neural retina. PMID: 17705196

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Involvement in disease
Defects in Crb1 are a cause of focal retinal dysplasia and degeneration associated with a shortening of inner and outer segments. Affected mice produce a secreted truncated protein that lacks the single transmembrane and the intracellular domain, and develop irregularities at the outer limiting membrane and loss of photoreceptor cells.
Subcellular Location
[Isoform 1]: Apical cell membrane; Single-pass type I membrane protein. Secreted. Cell projection, cilium, photoreceptor outer segment. Photoreceptor inner segment.; [Isoform 3]: Secreted. Cytoplasm. Cell junction, focal adhesion.
Protein Families
Crumbs protein family
Tissue Specificity
[Isoform 1]: Expressed in the kidney, lung, stomach and testis. Expressed in the brain. Expressed in the retina of the eye. Expressed in the outer nuclear layer, photoreceptor layer and inner nuclear layer of the retina. Expressed in Mueller cell radial p
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