Recombinant Mouse DNA-directed RNA polymerase III subunit RPC5 (Polr3e)

Code CSB-YP861500MO
MSDS
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Source Yeast
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Code CSB-EP861500MO
MSDS
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Source E.coli
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Code CSB-EP861500MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861500MO
MSDS
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Source Baculovirus
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Code CSB-MP861500MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Polr3e
Uniprot No.
Alternative Names
Polr3e; Sin; DNA-directed RNA polymerase III subunit RPC5; RNA polymerase III subunit 5; RNA polymerase III subunit C5; Sex-lethal interactor homolog; Sxl interactor
Species
Mus musculus (Mouse)
Expression Region
1-710
Target Protein Sequence
MANEEDDPVI QEIDVYLAKS LAEKLYLFQY PVRPASMTYD DIPHLSAKIK PKQQKVELEM AIDTLNPNYC RSKGEQIALN VDGACADETS TYSSKLMDKQ TFCSSQTTSN TARYAAALYR QGELHLTPLH GILQLRPSFS YLDKADAKHR EREAANEAGD SSQDEAEEDV KQITVRFSRP ESEQARQRRV QSYEFLQKKH AEEPWVHLHY YGMRDSRSEH ERQYLLCQGS SGVENTELVK SPSEYLMMLM PPSPEEEKDK PVAPSNVLSM AQLRTLPLAD QIKVLMKNVK VMPFANLMSL LGPSVDSVAV LRGIQKVAML VQGNWVVKSD ILYPKDSSSP HSGMPAEVLC RGRDFVMWKF TQSRWVVRKE VAAVTKLCAE DVKDFLEHMA VVRINKGWEF LLPYDLEFIK KHPDVVQRQH MLWSGIQAKL EKVYNLVKET MPKKPDGQSA PVGLVSGEQR VQTAKTKAQQ NHAFLERELQ RRKEQMRAAT VLPSVQIKEE PLSEEEADGA ELEAEEEEPM DTAPSTCLST KLANGLPAGR AVGGDSLNGH PVPGCASNPV ACELKAFVEA TFQRQFVLTL SELKRLFNLH LAGLPPGHIL FSGVSDRMLQ DTVLAAGCKQ ILVPFPPQTA ASPDEQKVFA LWESGDMSDQ HRQVLLEIFS KNYRVRRNLI QSRLTQECGE ELSKQEVDKV LKDCCVSCGG MWYLKGTVQS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific periphjeric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Essential for efficient transcription from both the type 2 VAI and type 3 U6 RNA polymerase III promoters. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts induce type I interferon and NF- Kappa-B through the RIG-I pathway.
Gene References into Functions
  1. a mammalian interspersed repeat (MIR) nested in antisense orientation within the first intron of the Polr3e gene, affects Polr3e expression through transcriptional interference PMID: 28289142
  2. Results identify Sin as a novel regulator of medullary thymic epithelial cell development and t cell tolerance. PMID: 20404100
Subcellular Location
Nucleus.
Database Links
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