Recombinant Mouse DNA repair protein XRCC3 (Xrcc3)

Code CSB-YP866624MO
MSDS
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Source Yeast
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Code CSB-EP866624MO
MSDS
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Source E.coli
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Code CSB-EP866624MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866624MO
MSDS
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Source Baculovirus
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Code CSB-MP866624MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Xrcc3
Uniprot No.
Alternative Names
Xrcc3; DNA repair protein XRCC3; X-ray repair cross-complementing protein 3
Species
Mus musculus (Mouse)
Expression Region
1-349
Target Protein Sequence
MDLDQLDLNP RITAAVKRGR LKSVKEILCY SGPDLQRLTG LPSHDVQCLL RAASLHLRGS RVLSALHLFQ QKESFPEQHQ RLSLGCPVLD QFLGGGLPLE GITGLAGCSS AGKTQLALQL CLAVQFPRQY GGLEAGAVYI CTEDAFPSKR LWQLIAQQRR LRTDAPEELI EKIRFSNHIF IEHAADVDTL LECVSKKVPI LLSRGMARLV VVDSIAAPFR CEFHLQASAI RAKLLLSLGA TLRRLSSTFR SPVLCINQVT DMVEDQQSVS RSLGASEERL SPALGITWAN QLLMRLMVDR THEDDVTTGL PRSPVRTLRV LFAPHLPLSS CCYTVSGEGI RGMPGTQSY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. Part of the RAD21 paralog protein complex CX3 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, CX3 acts downstream of RAD51 recruitment; the complex binds predominantly to the intersection of the four duplex arms of the Holliday junction (HJ) and to junctions of replication forks. Involved in HJ resolution and thus in processing HR intermediates late in the DNA repair process; the function may be linked to the CX3 complex and seems to involve GEN1 during mitotic cell cycle progression. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51 and RAD51C.
Gene References into Functions
  1. the RAD51C-XRCC3-associated Holliday junction resolvase complex associates with crossovers and may play an essential role in the resolution of recombination intermediates prior to chromosome segregation PMID: 17114795
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Mitochondrion matrix.
Protein Families
RecA family, RAD51 subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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