Recombinant Mouse DNA replication factor Cdt1 (Cdt1)

Code CSB-YP848129MO
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Source Yeast
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Code CSB-EP848129MO
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Source E.coli
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Code CSB-EP848129MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP848129MO
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Source Baculovirus
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Code CSB-MP848129MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cdt1
Uniprot No.
Alternative Names
Cdt1; Ris2DNA replication factor Cdt1; Double parked homolog; DUP; Retroviral insertion site 2 protein
Species
Mus musculus (Mouse)
Expression Region
1-557
Target Protein Sequence
MAQSRVTDFY ACRRPGLTTP RAKSICLTPS PGGLVAPAFT RSSSRKRARP PAEPGSDQPA PLARRRLRLP GLDSCPSSLP EPSSPAEPSP PADPSPPADP GSPVCPSPVK RTKSTTVYVG QQPGKIPSED SVSELQSCLR RARKLGAQAR ALRARVQENA VEPSTPDAKV PTEQPCVEKA PAYQRFHALA QPGLPGLVLP YKYQVLVEMF RSMDTIVSML HNRSETVTFA KVKQGVQEMM RKRFEERNVG QIKTVYPTSY RFRQECNVPT FKDSIKRSDY QLTIEPLLGQ EAGGATQLTA TCLLQRRQVF RQNLVERVKE QHKVFLASLN PPMAVPDDQL TRWHPRFNVD EVPDIEPAEL PQPPVTEKLT TAQEVLARAR SLMTPKMEKA LSNLALRSAE PGSPGTSTPP LPATPPATPP AASPSALKGV SQALLERIRA KEVQKQLARM TRCPEQELRL QRLERLPELA RVLRNVFVSE RKPALTMEVV CARMVDSCQT ALSPGEMEKH LVLLAELLPD WLSLHRIRTD TYVKLDKAVD LAGLTARLAH HVHAEGL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene.
Gene References into Functions
  1. conserved arginine residues play critical roles in interaction with Geminin and Mcm that are crucial for proper conformation of the complexes and its licensing activity. PMID: 26940553
  2. This study reveals a conserved new regulatory Cdt1 domain crucial for proper DNA licensing activity. PMID: 23817338
  3. Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins. PMID: 20335175
  4. Determined the structures of mCdt1CS (mCdt1C_small; residues 452 to 557) and mCdt1CL (mCdt1C_large; residues 420 to 557) using X-ray crystallography and NMR. This study reveals that Cdt1 is formed with a tandem repeat of the winged helix domain. PMID: 19722278
  5. Cdt1 function is negatively regulated by the Cdk phosphorylation independent of geminin binding PMID: 14993212
  6. In situ hybridization and immunohistochemistry localize Cdt1 as well as geminin to the proliferative compartment of the developing mouse gut epithelium PMID: 15291814
  7. Cdt1 expression characterizes progenitor cells in G1 phase PMID: 17533120
  8. DNA binding and helicase activities of Mcm4/6/7 are significantly stimulated by Cdt1 PMID: 18606811

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Subcellular Location
Nucleus. Chromosome, centromere, kinetochore.
Protein Families
Cdt1 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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