Recombinant Mouse Disks large homolog 1 (Dlg1), partial

Code CSB-YP773963MO
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Source Yeast
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Code CSB-EP773963MO
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Source E.coli
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Code CSB-EP773963MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP773963MO
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Source Baculovirus
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Code CSB-MP773963MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dlg1
Uniprot No.
Alternative Names
Dlg1; Dlgh1Disks large homolog 1; Embryo-dlg/synapse-associated protein 97; E-dlg/SAP97; Synapse-associated protein 97; SAP-97; SAP97
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential multidomain scaffolding protein required for normal development. Recruits channels, receptors and signaling molecules to discrete plasma membrane domains in polarized cells. Regulates the excitability of cardiac myocytes by modulating the functional expression of Kv4 channels. Functional regulator of Kv1.5 channel. May play a role in adherens junction assembly, signal transduction, cell proliferation, synaptogenesis and lymphocyte activation. During long-term depression in hippocampal neurons, it recruits ADAM10 to the plasma membrane.
Gene References into Functions
  1. we found that Dlg1 was expressed at different stages of oocyte development. at the germinal vesicle stage, Dlg1 was present in the cytoplasm, prominently surrounding the germinal vesicle membrane. During maturation, Dlg1 became highly polarised by associating with the spindle and formed characteristic crescent-shaped accumulations under the cortex. PMID: 27651179
  2. Adrenergic modulation of Kv1.1 was mediated by the signaling scaffold SAP97, which, through direct protein-protein interactions, escorts beta2 signaling to remove Kv1.1 from the dendrite surface. This process promoted spike timing-dependent long-term synaptic potentiation at mouse hippocampal excitatory synapses. PMID: 28103480
  3. that Pten, through the Dlg1-binding ability of its PDZ-binding domain , accumulates phosphorylated Eg5 at duplicated centrosomes to establish symmetrical bipolar spindles that properly segregate chromosomes PMID: 27240320
  4. Kif13b also negatively regulates Dlg1 stability in oligodendrocytes, in which Dlg1, in contrast to Schwann cells, enhances AKT activation and promotes myelination PMID: 27070899
  5. Dlg1 regulates p38-dependent proinflammatory cytokine production. PMID: 26186728
  6. phenotypes of Dlg1-/- mice involving cellular disorganization and insufficient tissue elongation strongly suggest a defect in the convergent extension movements PMID: 25860837
  7. Loss of Dlg1 confers strong predisposition to the development of pediatric B-ALL in a murine model. PMID: 24778134
  8. Loss of Dlg-1 in the mouse lens impairs fibroblast growth factor receptor signaling. PMID: 24824078
  9. lack of the Dlg1 gene induces a decrease in apoptotic epithelial cell death and the persistence of the common nephric duct, which result in a dysfunctional vesicoureteral junction and cause megaureter or hydronephrosis through vesicoureteral reflux PMID: 24142560
  10. DLG1 influences distal ureter maturation via a non-epithelial cell autonomous mechanism involving reduced retinoic acid signaling, Ret expression, and apoptosis. PMID: 24699546
  11. Synapse-associated protein 97 regulates the membrane properties of fast-spiking parvalbumin interneurons in the visual cortex in mice. PMID: 23904610
  12. Mice in which SAP97, the mammalian homolog of DlgS97, was conditionally deleted in adults failed to develop rapid tolerance to ethanol's sedative/hypnotic effects. PMID: 23145041
  13. These findings identify novel roles for Dlgh-1 in vertebrates that differ from its well-characterized roles in invertebrates and suggest that the Dlgh-1 null mouse may be a useful animal model to study certain human congenital birth defects. PMID: 23349879
  14. Dlg1 regulates the generation of memory T cells. PMID: 23436244
  15. T cells with acute Dlg1 suppression manifest differential requirements for Dlg1 activity in signaling events leading to Th1 versus Th2 cytokine induction. PMID: 23028902
  16. SAP97 promotes the stability of Nax channels at the plasma membrane. PMID: 23022437
  17. SAP97 accumulates and binds to IgG B-cell receptors in the immunological synapses that forms in response to B cell engagement with antigen. PMID: 22855505
  18. Membrane-associated guanylate kinase homolog SAP97 is a central organizer of postsynaptic density at excitatory synapses on pyramidal neurons. PMID: 21031555
  19. HSF-1 induced transcription of SAP97 through SIRT1-dependent interaction with HSEs; the increase in SAP97 resulted in stabilization of Kv1.5 channels PMID: 21232033
  20. SAP97 and dystrophin macromolecular complexes determine two pools of cardiac sodium channels Nav1.5 in cardiomyocytes. PMID: 21164104
  21. Interfering with SAP97/a disintegrin and metalloproteinase domain containing protein 10 (Adam10) complex shifts the metabolism of amyloid precursor protein toward amyloidogenesis allowing reproduction of initial phases of sporadic Alzheimer's disease. PMID: 20805102
  22. findings show that, in Schwann cells, Dlg1 interacts with PTEN to inhibit axonal stimulation of myelination; this mechanism limits myelin sheath thickness & prevents overmyelination in sciatic nerves PMID: 20448149
  23. These results support the hypothesis that SAP97 is part of the machinery that traffics glutamate receptors and compensates for other PSD-MAGUKs in knockout mouse models. PMID: 20133708
  24. CaMKIIalpha targets a specific SAP97 splice variant to disengage AKAP79/150 from regulating GluR1 AMPARs PMID: 19858198
  25. Results suggest that Dlgh1 coordinates T cell receptor/CD28-induced actin-driven T cell synapse assembly, signal transduction, and effector function. PMID: 15699074
  26. characterize the binding of the C terminus of the NR2B subunit of N-methyl-D-aspartate receptors with the PDZ domains of SAP97 and determine the structure of the PDZ1-NR2B complex PMID: 15929985
  27. a direct interaction between SAP97 and PSD-95 may play a functional role in the trafficking and clustering of AMPA receptors. PMID: 16332687
  28. The observed localization of Dlg-1 and Scrib with cadherins suggests that these proteins may play roles in cell adhesion, polarity and proliferation, as they do in invertebrates, suggesting cross-species conservation of function for these PDZ proteins. PMID: 16402019
  29. Dlgh1 also regulates smooth muscle orientation, and human DLG1 mutations may contribute to hereditary forms of hydronephrosis PMID: 17172448
  30. MAGUK scaffold proteins as 'orchestrators' of T cell receptor signal specificity PMID: 17187070
  31. Localization of Dlgh1 in livers from Cx32 null mice indicates that in the absence of Cx32, Dlgh1 is decreased, and the remainder is translocated from the hepatocyte membrane to the nucleus with some remaining in cytoplasmic compartments. PMID: 17284442
  32. These results suggest a novel role for Dlgh1 in regulating epithelial duct formation and morphogenesis during mammalian development. PMID: 17435047
  33. In T lymphocytes, DLGH1 functions as a negative regulator of T-cell receptor-induced proliferative responses. PMID: 17724087
  34. These results suggest that the interaction between Net1 and Dlg1 may contribute to the mechanism of Net1-mediated transformation. PMID: 17938206
  35. Results describe E-dlg, a mouse homologue of the Drosophila discs large tumor suppressor (Dlg, also known as SAP97), that binds preferentially to SAP102. PMID: 18618587
  36. This is the first study to demonstrate the involvement of Dlg1, Scrib, and Lgl1 in a mouse with ocular adenocarcinoma and the simultaneous involvement of these proteins in the same cancer. PMID: 19098995
  37. Interaction of Dlg1 with the Sec8 exocyst component promotes membrane addition, whereas with myotubularin-related protein 2 (Mtmr2), negatively regulates membrane formation. PMID: 19587293
  38. Results suggest that Dlg-1 acts in a cell autonomous manner to regulate epithelial cell structure and fiber cell differentiation. PMID: 19623611

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Subcellular Location
Membrane; Peripheral membrane protein. Basolateral cell membrane. Endoplasmic reticulum membrane. Cell junction, synapse, postsynaptic density. Cell junction, synapse. Cell membrane, sarcolemma. Cell junction. Cytoplasm. Apical cell membrane.
Protein Families
MAGUK family
Tissue Specificity
Expressed in the brain (at protein level). Expressed in the outer limiting membrane and outer plexiform layer in the retina (at protein level). Expressed in epithelial, mesenchymal, neuronal, endothelial and hematopoietic cells during embryogenesis. Expre
Database Links

KEGG: mmu:13383

STRING: 10090.ENSMUSP00000064280

UniGene: Mm.382

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