Product Details

Purity

>85% (SDS-PAGE)

Target Names

Dnajc3

Uniprot No.

Q91YW3

Alternative Names

Dnajc3; P58ipkDnaJ homolog subfamily C member 3; Interferon-induced; double-stranded RNA-activated protein kinase inhibitor; Protein kinase inhibitor of 58 kDa; Protein kinase inhibitor p58

Species

Mus musculus (Mouse)

Expression Region

32-504

Target Protein Sequence

GVNADVEKH LELGKKLLAA GQLADALSQF HAAVDGDPDN YIAYYRRATV FLAMGKSKAA LPDLTKVIAL KMDFTAARLQ RGHLLLKQGK LDEAEDDFKK VLKSNPSEQE EKEAESQLVK ADEMQRLRSQ ALDAFDGADY TAAITFLDKI LEVCVWDAEL RELRAECFIK EGEPRKAISD LKAASKLKSD NTEAFYKIST LYYQLGDHEL SLSEVRECLK LDQDHKRCFA HYKQVKKLNK LIESAEELIR DGRYTDATSK YESVMKTEPS VAEYTVRSKE RICHCFSKDE KPVEAIRICS EVLQMEPDNV NALKDRAEAY LIEEMYDEAI QDYEAAQEHN ENDQQIREGL EKAQRLLKQS QKRDYYKILG VKRNAKKQEI IKAYRKLALQ WHPDNFQNEE EKKKAEKKFI DIAAAKEVLS DPEMRKKFDD GEDPLDAESQ QGGGGNPFHR SWNSWQGFNP FSSGGPFRFK FHFN

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Involved in the unfolded protein response (UPR) during endoplasmic reticulum (ER) stress. Acts as a negative regulator of the EIF2AK4/GCN2 kinase activity by preventing the phosphorylation of eIF-2-alpha at 'Ser-52' and hence attenuating general protein synthesis under ER stress, hypothermic and amino acid starving stress conditions. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.

Gene References into Functions

Chaperone protein p58(IPK) is expressed predominantly in retinal ganglion cells (RGC), inner retinal neurons, and the photoreceptor inner segments. PMID: 25655802
p58IPK is a general inhibitor of the eIF2alpha kinases in that it also interacts with GCN2. Thus forced overexpression of cytoplasmic p58 delays eIF2alpha phosphorylation, suppresses GCN2 phosphorylation and prolongs protein synthesis PMID: 25329545
Data present a working model for P58(IPK) to act together with Bip to prevent protein aggregations and promote protein foldings within ER. PMID: 21266255
Coxsackievirus B3 infection induces apoptosis through downregulation of p58IPK and activation of CHOP and SREBP1. PMID: 20554776
mechanism by which P58(IPK) functions to promote protein folding within the endoplasmic reticulum, determined from the crystal structure of P58(IPK) PMID: 20184891
renal injury inflicted by expression of the folding mutants was markedly enhanced by haploinsufficiency of the endoplasmic reticulum cochaperone p58(IPK). PMID: 20308551
The stress of unfolded proteins in the ER activates P58(IPK) transcription through an ER stress-response element in its promoter. P58(IPK) interacts with & inhibits PERK, which protects cells during the ER-stress response by reducing protein synthesis. PMID: 12446838
Insulin deficiency associated with the absence of P58(IPK) mimics beta-cell failure associated with type 1 and late-stage type 2 diabetes. PMID: 15793246
absence of P58(IPK) led to an increase in eIF2alpha phosphorylation and decreased influenza virus mRNA translation PMID: 17166899
These results identify a previously unanticipated location for p58(IPK) in the ER lumen where its putative function as a cochaperone explains the stress-sensitivity phenotype of knockout cells and mice. PMID: 17567950
investigated the mechanism by which P58(IPK) functions to promote protein folding within the ER, cy crystallizing a P58(IPK) TPR fragment without the C-terminal J-domain PMID: 18259061
Selective and stable binding to misfolded proteins by P58's TPR-containing N-terminal domain, is reported. PMID: 18923430
Results show that P58(IPK) is activated during virus infection to inhibit virus-induced apoptosis and inflammation to prolong host survival, even while prolonging viral replication. PMID: 19461876

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Subcellular Location

Endoplasmic reticulum.

Tissue Specificity

Widely expressed, with high level in the liver.

Database Links

KEGG: mmu:100037258

STRING: 10090.ENSMUSP00000022734

UniGene: Mm.12616

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Recombinant Mouse DnaJ homolog subfamily C member 3 (Dnajc3)

Product Details

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Target Background

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