Recombinant Mouse Dual specificity protein phosphatase 7 (Dusp7)

Code CSB-YP842055MO
MSDS
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Source Yeast
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Code CSB-EP842055MO
MSDS
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Source E.coli
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Code CSB-EP842055MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842055MO
MSDS
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Source Baculovirus
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Code CSB-MP842055MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dusp7
Uniprot No.
Alternative Names
Dusp7Dual specificity protein phosphatase 7; EC 3.1.3.16; EC 3.1.3.48
Species
Mus musculus (Mouse)
Expression Region
1-422
Target Protein Sequence
MKNQLRGPPV RAHMSTSGAA AAAAAGGTRA GSEPGAGSGS GAGIGAGATT GAGAMPCKSA EWLQEELEAR GGASLLLLDC RPHELFESSH IETAINLAIP GLMLRRLRKG NLPIRSIIPN HADKERFATR CKAATVLLYD EATAEWQPEP GAPASVLGLL LQKLRDDGCQ AYYLQGGFNK FQTEYSEHCE TNVDSSSSPS GSPPTSVLGL GGLRISSDCS DGESDRELPS SATESDGSPV PSSQPAFPVQ ILPYLYLGCA KDSTNLDVLG KYGIKYILNV TPNLPNAFEH GGEFTYKQIP ISDHWSQNLS QFFPEAISFI DEARSKKCGV LVHCLAGISR SVTVTVAYLM QKMNLSLNDA YDFVKRKKSN ISPNFNFMGQ LLDFERTLGL SSPCDNHAPS EQLYFSTPTN HNLFPINTLE ST
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Dual specificity protein phosphatase. Shows high activity towards MAPK1/ERK2. Also has lower activity towards MAPK14 and MAPK8. In arrested oocytes, plays a role in meiotic resumption. Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB. May also inactivate PRKCA and/or PRKCG. Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex.
Gene References into Functions
  1. DUSP7 is an essential regulator of multiple steps in oocyte meiosis. PMID: 27783954
  2. Data show that Dusp7 transcripts is expressed in partially overlapping patterns that correspond to regions of active FGF signaling, suggesting combinatorial roles in negative regulation of this pathway during ear development. PMID: 18058922
Subcellular Location
Cytoplasm.
Protein Families
Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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