Recombinant Mouse E3 ubiquitin-protein ligase pellino homolog 1 (Peli1)

Code CSB-YP804464MO
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Source Yeast
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Code CSB-EP804464MO
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Source E.coli
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Code CSB-EP804464MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804464MO
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Source Baculovirus
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Code CSB-MP804464MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Peli1
Uniprot No.
Alternative Names
Peli1; E3 ubiquitin-protein ligase pellino homolog 1; Pellino-1; EC 2.3.2.27; RING-type E3 ubiquitin transferase pellino homolog 1
Species
Mus musculus (Mouse)
Expression Region
1-418
Target Protein Sequence
MFSPDQENHP SKAPVKYGEL IVLGYNGSLP NGDRGRRKSR FALFKRPKAN GVKPSTVHIA CTPQAAKAIS NKDQHSISYT LSRAQTVVVE YTHDSNTDMF QIGRSTESPI DFVVTDTVPG SQSNSDTQSV QSTISRFACR IICERSPPFT ARIYAAGFDS SKNIFLGEKA AKWKTSDGQM DGLTTNGVLV MHPRNGFTED SKPGIWREIS VCGNVFSLRE TRSAQQRGKM VEIETNQLQD GSLIDLCGAT LLWRTAEGLS HTPTVKHLEA LRQEINAARP QCPVGFNTLA FPSMKRKDVV DEKQPWVYLN CGHVHGYHNW GNKEERDGKD RECPMCRSVG PYVPLWLGCE AGFYVDAGPP THAFSPCGHV CSEKTTAYWS QIPLPHGTHT FHAACPFCAH QLAGEQGYIR LIFQGPLD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Involved in the TLR and IL-1 signaling pathways via interaction with the complex containing IRAK kinases and TRAF6. Mediates 'Lys-63'-linked polyubiquitination of IRAK1 allowing subsequent NF-kappa-B activation. Mediates 'Lys-48'-linked polyubiquitination of RIPK3 leading to its subsequent proteasome-dependent degradation; preferentially recognizes and mediates the degradation of the 'Thr-182' phosphorylated form of RIPK3. Negatively regulates necroptosis by reducing RIPK3 expression. Mediates 'Lys-63'-linked ubiquitination of RIPK1.
Gene References into Functions
  1. Peli1 has a B cell-intrinsic function to protect against lupus-like autoimmunity. Peli1 deficiency in B cells induces autoantibody production via noncanonical NF-kappaB signaling. Mechanically, Peli1 functions as an E3 ligase to associate with NF-kappaB inducing kinase (NIK) and mediates NIK Lys48 ubiquitination and degradation. PMID: 29555915
  2. This study demonstrates Pellino 1 (PELI1) as an important modulator that exerts opposite regulatory functions on apoptosis and necroptosis, two distinct forms of regulated cell death mechanisms. PMID: 29078411
  3. Peli1 is a proangiogenic molecule that acts downstream of VEGF-Flk-1 and restores angiogenesis and enhances skin flap survivability. PMID: 28177171
  4. a novel miR-155-Peli1-c-Rel pathway that specifically regulates Tfh cell generation and function. PMID: 27481129
  5. Pellino1 plays an important role in the pathogenesis of myocardial infarction PMID: 24442869
  6. The nonredundant role of Pellino1 in immune receptor signaling is in part due to its different substrate specificity. PMID: 25027698
  7. Pellino1 interacts with the transcription factor Deformed Epidermal Autoregulatory Factor 1 (DEAF1). PMID: 23846693
  8. These results demonstrate that the ligands that signal via MyD88 do not always employ the same protein kinase to activate Pellino 1 and show that the activiation of NF-kappaB and mitogen-activated protein kinases. PMID: 22007846
  9. Peli1 as a critical factor in the maintenance of peripheral T cell tolerance and demonstrate a previously unknown mechanism of c-Rel regulation. PMID: 21874024
  10. Pellino-1 protein may have a cellular function distinct from previously identified functions. PMID: 21120624
  11. miR-21 expression was upregulated during early stages of liver regeneration. Targeting of Peli1 by miR-21 could potentially provide the basis for a negative feedback cycle regulating NF-kappaB signaling. PMID: 20167875
  12. Peli1 is a ubiquitin ligase needed for the transmission of TRIF-dependent TLR signals. PMID: 19734906

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Protein Families
Pellino family
Database Links
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