Recombinant Mouse ERC protein 2 (Erc2), partial

Code CSB-YP750875MO
MSDS
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Source Yeast
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Code CSB-EP750875MO
MSDS
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Source E.coli
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Code CSB-EP750875MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP750875MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP750875MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Erc2
Uniprot No.
Alternative Names
Erc2; Cast1; D14Ertd171e; Kiaa0378ERC protein 2; CAZ-associated structural protein 1; CAST1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Thought to be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Seems to act together with BSN. May recruit liprin-alpha proteins to the CAZ.
Gene References into Functions
  1. CAST supports large active zone size and high rates of transmission at rod ribbon synapses, which are required for normal vision. PMID: 22933801
  2. CAST (cytomatrix at the active zone structural protein) playes pivotal roles in both presynaptic regulation of quantal size and recycling of endocytosed synaptic vesicles. PMID: 27422015
  3. CAST/SAD-B reaction serves as a brake on synaptic transmission PMID: 27626661
  4. the direct binding of CAST and the voltage-dependent Ca(2+) channel beta4-subunit (VDCC) regulates the opening of the functional VDCC complex PMID: 22577167
  5. The ELKS2alpha is essential for mediating neurotransmitter release, our results suggest that ELKS2alpha normally restricts release and limits the size of the readily-releasable pool of synaptic vesicles at the active zone of inhibitory synapses. PMID: 19874790

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Subcellular Location
Cytoplasm. Cell junction, synapse. Cell junction, synapse, presynaptic active zone. Cytoplasm, cytoskeleton. Note=Localized to the active zone of presynaptic density.
Tissue Specificity
Expressed throughout the central nervous system, including hippocampus, cortex, cerebellum and olfactory bulb.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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