Recombinant Mouse Ecto-NOX disulfide-thiol exchanger 1 (Enox1)

Code CSB-YP007676MO
MSDS
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Source Yeast
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Code CSB-EP007676MO
MSDS
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Source E.coli
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Code CSB-EP007676MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007676MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP007676MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Enox1
Uniprot No.
Alternative Names
Enox1Ecto-NOX disulfide-thiol exchanger 1; Constitutive Ecto-NOX; cNOX) [Includes: Hydroquinone [NADH] oxidase; EC 1.-.-.-); Protein disulfide-thiol oxidoreductase; EC 1.-.-.-)]
Species
Mus musculus (Mouse)
Expression Region
1-643
Target Protein Sequence
MVDAAGFESV AQCPRELHQM MAAAADGLGS IALDTTQLNM SVTDPTAWAT AMNNLGMVPV GLPGQQLVSD SICVPGFDPG LNMMTGITPI NPMIPGLGLV PPPPPTEVAV VKEIIHCKSC TLFPQNPNLP PPSTRERPPG CKTVFVGGLP ENATEEIIQE VFEQCGDITA IRKSKKNFCH IRFAEEFMVD KAIYLSGYRM RLGSSTDKKD SGRLHVDFAQ ARDDFYEWEC KQRMRAREER HRRKLEEDRL RPPSPPAIMH YSEHEAALLA DKLKDDSKFS EAITVLLSWI ERGEVNRRSA NQFYSMVQSA NSHVRRLMNE KATHEQEMEE AKENFKNALT GILTQFEQIV AVFNASTRQK AWDHFSKAQR KNIDIWRKHS EELRNAQSEQ LMGIRREEEM EMSDDENCDS PTKKMRVDES ALAAQAYALK EENDSLRWQL DAYRNEVELL KQEKEQLFRT EENLTKDQQL QFLQQTMQGM QQQLLAIQEE LNNKKSELEQ AKEEQSHTQA LLKVLQEQLK GTKDLVETNG HSHEDANEIN VLTVALVNQD RENNTEKRSQ GLKSEKEALL IGIISTFLHV HPFGANIEYL WSYMQQLDSK ISANEIEMLL MRLPRMFKQE FTGVGATLEK RWKLCAFEGI KTT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probably acts as a terminal oxidase of plasma electron transport from cytosolic NAD(P)H via hydroquinones to acceptors at the cell surface. Hydroquinone oxidase activity alternates with a protein disulfide-thiol interchange/oxidoreductase activity which may control physical membrane displacements associated with vesicle budding or cell enlargement. The activities oscillate with a period length of 24 minutes and play a role in control of the ultradian cellular biological clock.
Gene References into Functions
  1. Enox1 in tumor stroma significantly enhances the effectiveness of 2 Gy fractionated radiotherapy and identifies Enox1 as a potential therapeutic target. PMID: 27788492
  2. Lithium at physiological concentrations induces an ECTO-NOX activity previously inactive from plant (soybean), murine (3T3 cells) and human (HUVEC and HeLa cells) sources and resets the period of the constitutive CNOX. PMID: 18462883
Subcellular Location
Cell membrane. Secreted, extracellular space.
Protein Families
ENOX family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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