Recombinant Mouse Endonuclease III-like protein 1 (Nthl1)

Code CSB-YP016125MO
MSDS
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Source Yeast
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Code CSB-EP016125MO
MSDS
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Source E.coli
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Code CSB-EP016125MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016125MO
MSDS
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Source Baculovirus
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Code CSB-MP016125MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nthl1
Uniprot No.
Alternative Names
Nthl1; Nth1; Endonuclease III-like protein 1; EC 3.2.2.-; EC 4.2.99.18; Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase; DNA glycosylase/AP lyase
Species
Mus musculus (Mouse)
Expression Region
1-300
Target Protein Sequence
MNSGVRMVTR SRSRATRIAS EGCREELAPR EAAAEGRKSH RPVRHPRRTQ KTHVAYEAAN GEEGEDAEPL KVPVWEPQNW QQQLANIRIM RSKKDAPVDQ LGAEHCYDAS APPKVRRYQV LLSLMLSSQT KDQVTAGAMQ RLRARGLTVE SILQTDDDTL GRLIYPVGFW RNKVKYIKQT TAILQQRYEG DIPASVAELV ALPGVGPKMA HLAMAVAWGT ISGIAVDTHV HRIANRLRWT KKMTKTPEET RKNLEEWLPR VLWSEVNGLL VGFGQQICLP VHPRCQACLN KALCPAAQDL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines.
Gene References into Functions
  1. These results suggest that Nth1 plays an important role in telomere maintenance and base repair against oxidative stress-induced base modifications. PMID: 23874233
  2. deficiencies in Ogg1 and/or Nth1 do not lead to decreased DNA double strand breaks PMID: 22536455
  3. To determine the significance of thymine glycol repair in mammals, a mouse model with mutated mNth1, a homolog of nth, was made by gene targeting. Mutant mouse liver, instead of mNTH1 activity, had at least 2 novel DNA glycosylase activities against Tg. PMID: 12093749
  4. To determine the role of murine Nth1 in protecting genomic integrity, a knockout strain was created. No phenotypic changes from wild type were noted due to a novel AP endonuclease DNA repair system. PMID: 12167705
  5. NEIL1 is a back-up glycosylase for NTH1 with unique substrate specificity and tissue-specific expression PMID: 12200441
  6. the mechanism for the removal of 5,6-dihydrouracil from DNA by mNTH1-deficient cell-free extracts derived from testes of mNTH1 knock-out mice PMID: 12401779
  7. mNTH1 is responsible for the repair of thymine glycols in mitochondrial DNA PMID: 12819227
  8. ability of nth1 to discriminate between thymine glycol stereoisomers PMID: 14726482
  9. results indicated that 8-oxoguanine DNA-glycosylase (OGG1) and nth (endonuclease III)-like 1(NTH1) are the major DNA glycosylases for the removal of 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyG) and 4,6-diamino-5-formamidopyrimidine (FapyA) PMID: 16221681

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Subcellular Location
Nucleus. Mitochondrion.
Protein Families
Nth/MutY family
Tissue Specificity
Widely expressed.
Database Links
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