Product Details

Purity

>85% (SDS-PAGE)

Target Names

Xpo1

Uniprot No.

Q6P5F9

Alternative Names

Xpo1; Crm1; Exportin-1; Exp1; Chromosome region maintenance 1 protein homolog

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Mediates the nuclear export of cellular proteins (cargos) bearing a leucine-rich nuclear export signal (NES) and of RNAs. In the nucleus, in association with RANBP3, binds cooperatively to the NES on its target protein and to the GTPase Ran in its active GTP-bound form. Docking of this complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the cargo from the export receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Involved in U3 snoRNA transport from Cajal bodies to nucleoli. Binds to late precursor U3 snoRNA bearing a TMG cap.

Gene References into Functions

These results suggest a differential interaction between human Crm1 and mouse Crm1 and many lentiviral Rev proteins, which may partially explain the HIV replicative defect in mice. PMID: 29028476
Data show that administration of a single dose of selinexor bound Exportin 1 (XPO1/CRM1) for minimally 72 hours both in vitro and in vivo. PMID: 26654943
These results suggest a model wherein HIV-1 Rev-associated nuclear export signals cooperate to regulate the number or quality of CRM1's interactions with viral Rev/RRE ribonucleoprotein complexes in the nucleus. PMID: 25275125
map the nuclear import and export signals of Dp71d by truncation and point mutant analysis, showing for the first time Dp71d shuttles between nucleus and cytoplasm mediated by conventional nuclear transporters, importin (IMP) alpha/beta and the exportin CRM1 PMID: 24486332
AKT3 controls mitochondrial biogenesis and autophagy via regulation of the major nuclear export protein CRM-1. PMID: 24081905
A CRM1-mediated nuclear export signal is essential for cytoplasmic localization of neurogenin 3 in neurons. PMID: 23383123
Bioavailable CRM1 inhibitor KPT-251 significantly inhibited renal cell carcinoma growth in vivo with the expected on target effects and no obvious toxicity. PMID: 23079374
CRM1 augments production of infectious human and feline immunodeficiency viruses from murine cells PMID: 22933280
CaMKI vies with CRM1/exportin 1 for access to a nuclear export signal, and assembly of a CaMKI-14-3-3 zeta-CCTalpha complex is a key effector mechanism that drives nuclear CCTalpha translocation. PMID: 22621903
Transcription-independent role of Bach1 in mitosis through a nuclear exporter Crm1-dependent mechanism. PMID: 22289179
CRM1 protein-mediated regulation of nuclear clusterin (nCLU), an ionizing radiation-stimulated, Bax-dependent pro-death factor PMID: 21953454
UAP56 and URH49 exhibit an intrinsic CRM1-independent nucleocytoplasmic shuttling PMID: 21799930
p53 subcellular localization resulting from CRM1 alterations may play an important role in lung tumorigenesis PMID: 21519798
These results clearly demonstrate that Nup98 functions as a novel shuttling cofactor for Crm1-mediated nuclear export in conjunction with RanBP3. PMID: 20375145
Crm1 inhibition promotes accumulation of p65 NF-kB in nuclei of poly(ADP-ribose) polymerase-1-deficient cells and reverses expression of NF-kappaB-dependent genes upon Toll-like receptor (TLR)4 stimulation. PMID: 20610652
results indicate that the minute virus of mice (MVMi) NS2-CRM1 interaction is an important determinant of MVMi virulence that can be modulated in nature PMID: 15367634
C-terminal sequences direct cyclin D1-CRM1 binding PMID: 15513923
NPM/B23 localizes between the paired centrioles of unduplicated centrosomes; inhibition of Crm1 nuclear export receptor results in both accumulation of cyclin E at centrosomes and efficient dissociation of NPM/B23 from centrosomes PMID: 16297385
Induction of differentiation in epidermal keratinocytes activates a specific program for post-transcriptional downregulation of E2F1, which involves signaling through p38 and activation of CRM1-dependent nuclear export PMID: 16924238
These results strongly suggest that CRM1 mediated-nuclear export of HDAC7 is independent of HDAC7 phosphorylation and its association with 14-3-3s. PMID: 16956611
PTH1R shuttles from the nucleus to the cytoplasm under normal physiological conditions and that this nuclear-cytoplasmic transport is dependent upon importin-alpha/beta and CRM1 PMID: 17317770
Inhibition of exportin-1-mediated nuclear export slowed down nucleocytoplasmic shuttling of v-Src-activated STAT3 resulting in reduced tyrosine phosphorylation, decreased induction of STAT3 target genes and increased apoptosis. PMID: 17726064
Phosphorylation of ChREBP was essential for its interaction with CRM1 for export to the cytosol, whereas nuclear import of ChREBP requires dephosphorylated ChREBP to interact with importin alpha. PMID: 18606808
These studies demonstrate the importance for keratinocyte proliferation of ILK regulation through changes in its subcellular localization, and establish ILKAP and CRM1 as pivotal modulators of ILK subcellular distribution and activity in these cells. PMID: 18635968
TRalpha may follow a cooperative export pathway in which both calreticulin and CRM1 play a role in facilitating efficient translocation of TRalpha from the nucleus to cytoplasm PMID: 18641393
study presents the crystal structure of the SPN1.CRM1.RanGTP export complex at 2.5 angstrom resolution (where SPN1 is snurportin1 and RanGTP is guanosine 5' triphosphate-bound Ran PMID: 19389996

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Subcellular Location

Cytoplasm. Nucleus, nucleoplasm. Nucleus, Cajal body. Nucleus, nucleolus.

Protein Families

Exportin family

Database Links

KEGG: mmu:103573

STRING: 10090.ENSMUSP00000020538

UniGene: Mm.217547

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Code	CSB-YP750809MO
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP750809MO
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP750809MO-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP750809MO
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP750809MO
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Recombinant Mouse Exportin-1 (Xpo1), partial

Product Details

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Target Background

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