Recombinant Mouse F-actin-capping protein subunit alpha-3(Capza3)

Code CSB-YP004513MO
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Source Yeast
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Code CSB-EP004513MO
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Source E.coli
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Code CSB-EP004513MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004513MO
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Source Baculovirus
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Code CSB-MP004513MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Capza3
Uniprot No. P70190
Alternative Names Capza3; Cappa3; Gsg3F-actin-capping protein subunit alpha-3; CapZ alpha-3; Germ cell-specific protein 3
Species Mus musculus (Mouse)
Expression Region 1-299
Target Protein Sequence MSLSVLSRKE KEKVIHRLLV QAPPGEFVNA FDDLCLLIRD EKLMHHQGEC AGHQHCQKYC VPLCIDGNPV LLSHHNVMGD FRFFDYQSKL SFRFDLLQNQ LRDIQSHGII RNETEYLRSV VMCALKLYVN DHYPNGNCNV LRKTVKSKEF LIACIEDHSY DNGECWNGLW KSKWIFQVNP FLTQVTGRIF VQAHFFRCVN LHIEVSKDLK ESLEVVNQAQ LALSFARLVE EQENKFQAAV IEELQELSNE ALRKILRRDL PVTRTLIDWQ RILSDLNLVM YPKLGYVIYS RSVLCNWII
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. May play a role in the morphogenesis of spermatid.
Gene References into Functions
  1. to understand actin-mediated change in protein localization during acrosome reaction, distribution of CAPZA3 was analyzed; like IZUMO1, CAPZA3 shows dynamic pattern of localization; movements follow different temporal pattern than changes seen with IZUMO1 PMID: 20458735
  2. CPalpha3 might play an important role in sperm morphogenesis and/or sperm function PMID: 18645673
  3. These observations strongly suggest the missense mutation in Capza3 is responsible for the mutant phenotype of repro32/repro32 sperm and regulation of F-actin dynamics PMID: 19341723
Protein Families F-actin-capping protein alpha subunit family
Tissue Specificity Exclusively expressed in the testis.
Database Links

KEGG: mmu:12344

STRING: 10090.ENSMUSP00000038562

UniGene: Mm.12817

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