Recombinant Mouse G-protein coupled bile acid receptor 1 (Gpbar1), partial

Code CSB-YP773890MO1
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Source Yeast
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Code CSB-EP773890MO1
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Source E.coli
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Code CSB-EP773890MO1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP773890MO1
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Source Baculovirus
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Code CSB-MP773890MO1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Gpbar1
Uniprot No.
Alternative Names
Gpbar1; Tgr5; G-protein coupled bile acid receptor 1; Membrane-type receptor for bile acids; M-BAR
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids. Involved in bile acid promoted GLP1R secretion.
Gene References into Functions
  1. G-protein coupled bile acid receptor 1 (TGR5) is required for cold-induced beiging of subcutaneous white adipose tissue (scWAT). PMID: 29339725
  2. Work is the first to provide evidence for a TGR5-inhibited inflammatory response in ischemia/reperfusion injury through suppression of the TLR4-NF-kappaB pathway. PMID: 27597295
  3. contributes to hepatic cystogenesis by increasing cAMP and enhancing cholangiocyte proliferation PMID: 28543567
  4. findings show GPBAR1 is essential for maintaining intestinal immune homeostasis and that its activation in the setting of inflammation reverses the immune dysfunction that occurs in rodent models of colitis PMID: 28607110
  5. Data suggest that FXR and TGR5 expression is down-regulated in aging kidney; caloric restriction prevents these age-related changes. Additionally, in long-lived Ames dwarf mice, renal FXR and TGR5 expression is up-regulated. Treatment of aged mice with dual FXR/TGR5 agonist reverses age-related changes in kidney structure/function. (FXR = farnesoid X activated receptor; TGR5 = G protein-coupled bile acid receptor 1) PMID: 28596381
  6. GPBAR1/TGR5 receptor agonist, tauroursodeoxycholic acid, has anti-inflammatory effects in microglial cells. PMID: 27987324
  7. Vertical sleeve gastrectomy achieves its postoperative therapeutic effects through enhanced TGR5 signaling. PMID: 27312543
  8. These results suggest that TGR5 contributes to the glucoregulatory benefits of vertical sleeve gastrectomy surgery by promoting metabolically favourable shifts in the circulating bile acid pool. PMID: 26511794
  9. findings uncovered a novel mechanism in which INT-767 activation of FXR induces Tgr5 gene expression and increases Ca(2+) levels and cAMP activity to stimulate GLP-1 secretion and improve hepatic glucose and lipid metabolism in high-fat diet-induced obese mice. PMID: 28478385
  10. The results suggest that TGR5 activation mediates cross-talk between alpha- and beta-cells by switching from glucagon to GLP-1 to restore beta- cell mass and function under hyperglycemic conditions. PMID: 26757816
  11. TGR5 is an important mediator of bile acid-induced cholangiocyte proliferation in vivo and in vitro. Furthermore, TGR5 protects cholangiocytes from death receptor-mediated apoptosis. PMID: 26420419
  12. miR-26a is a target gene of bile acid receptor GPBAR-1/TGR5 PMID: 26107166
  13. Impaired Itching Perception in Murine Models of Cholestasis Is Supported by Dysregulation of GPBAR1 Signaling PMID: 26177448
  14. Data show that the action of bile acids on -like peptide-1 (GLP-1) secretion is predominantly mediated by G protein-coupled bile acid receptor GPBAR1 (TGR5) located on the basolateral L-cell membrane. PMID: 26280129
  15. TGR5 appears to be crucial for protecting the regenerating liver from bile acid overload. PMID: 26045264
  16. Activation of TGR5 alleviates neuroinflammation and improves outcome in hepatic encephalopathy. PMID: 26179031
  17. XGB increases BMR by TGR5-dependent mechanisms in mice PMID: 25738495
  18. these studies reveal a signaling pathway downstream of TGR5 that modulates chemokine expression in response to high-fat diet PMID: 25365223
  19. Bile acids induce pruritus in mice by co-activation of TGR5 and TRPA1. PMID: 25194674
  20. TGR5 mediates the bile acid-induced pro-inflammatory cytokine production in murine Kupffer cells. PMID: 24755711
  21. TGR5 is a critical element in testicular pathophysiology. PMID: 24798773
  22. GPBAR1 is essential to maintain gastric and intestinal mucosal integrity. GPBAR1 agonists protect against gastrointestinal injury caused by ASA and NSAIDs by a COX-independent mechanism. PMID: 22881598
  23. Stimulation of GPBAR1 enhances endothelial barrier function via activation of protein kinase A and Rac1. PMID: 24144793
  24. The receptor TGR5 protects the liver from bile acid overload during liver regeneration in mice. PMID: 23686672
  25. TGR5 agonism induces NO production via Akt activation and intracellular Ca(2+) increase in vascular endothelial cells, and this function inhibits monocyte adhesion in response to inflammatory stimuli. PMID: 23619297
  26. FXR/TGR5 activation coordinates the immune phenotype of monocytes and macrophages, both in vitro and in vivo, identifying potential targeting strategies for treatment of NAFLD. PMID: 23460643
  27. Bile acids activate TGR5 on sensory nerves, stimulating the release of neuropeptides in the spinal cord that transmit itch and analgesia. PMID: 23524965
  28. TGR5 receptor activation on smooth muscle reveals a novel mechanism for the regulation of gut motility by bile acids PMID: 23275618
  29. this study demonstrates that the TGR5 expressed in the pancreatic beta cells regulates insulin secretion and highlights the importance of ongoing therapeutic strategies targeting TGR5 in the control of glucose homeostasis. PMID: 23022524
  30. The receptor TGR5 mediates the effects of bile acids on colonic motility, and deficiency of TGR5 causes constipation in mice. PMID: 23041323
  31. Data suggest that TGR5 represents an essential component in the pathway mediating the enhanced GLP-1 release in response to anionic exchange resins. PMID: 22666533
  32. Bile acid binding resin and bile acid have similar effects on bile acid composition and thermogenesis, suggesting that they both are mediated via TGR5 activation PMID: 22952571
  33. TGR5 activation yields a significant immune modulating function in the prevention of atherosclerosis. PMID: 22152303
  34. GP-BAR1 regulates intestinal barrier structure. Its expression increases in rodent models of colitis and Crohn's disease. Ciprofloxacin is a GP-BAR1 ligand. PMID: 22046243
  35. These findings identify TGR5 as a negative mediator of inflammation that may serve as an attractive therapeutic tool for immune and inflammatory liver diseases. PMID: 21735468
  36. The G protein-coupled bile acid receptor, TGR5, stimulates gallbladder filling PMID: 21454404
  37. Activation of GPBAR1 receptors by hydrophobic bile salts leads to the opening of potassium K(ATP) channels. PMID: 20624794
  38. The TGR5 is localized in the primary cilium of mouse cholangiocytes and the receptor could play an important role in coupling biliary bile acid concentration and composition to ductular bile formation. PMID: 20623999
  39. Bile acids activate GpBAR1 on inhibitory motor neurons to release nitric oxide and suppress motility PMID: 20236244
  40. The discovery of the bile acid receptor, GpBAR1, represented a major step forward in our understanding of how cells can sense and respond to bile acids--{REVIEW} PMID: 20553563
  41. There is a gender-dependent regulation of Gpbar1 function in metabolic disease. PMID: 20354075
  42. Gpbar1 may play a critical role in the evolution of bile-acid-induced pancreatitis by coupling exposure to bile acids with generation of pathological intracellular calcium transients, intra-acinar cell zymogen activation, and acinar cell injury. PMID: 19900448
  43. TGR5 is implicated in the suppression of macrophage functions by bile acids PMID: 12524422
  44. bile acids induce GLP-1 secretion through TGR5 and intracellular cAMP production in STC-1 cells PMID: 15721318
  45. Gpbar1 plays a critical role in the formation of gallstones, possibly via a regulatory mechanism involving the cholesterol 7alpha-hydroxylase pathway. PMID: 16724960
  46. Our data suggest that both oleuropein and oleanolic acid are involved in the anti-diabetic effect of olive leaves and further emphasize the potential role of TGR5 agonists to improve metabolic disorders. PMID: 17825251
  47. TGR5 signaling induces intestinal glucagon-like peptide-1 release, leading to improved liver and pancreatic function and enhanced glucose tolerance in obese mice. PMID: 19723493

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Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
G-protein coupled receptor 1 family
Database Links
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