Recombinant Mouse Geranylgeranyl transferase type-1 subunit beta (Pggt1b)

Code CSB-YP806437MO
MSDS
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Source Yeast
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Code CSB-EP806437MO
MSDS
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Source E.coli
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Code CSB-EP806437MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806437MO
MSDS
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Source Baculovirus
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Code CSB-MP806437MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pggt1b
Uniprot No.
Alternative Names
Pggt1b; Geranylgeranyl transferase type-1 subunit beta; EC 2.5.1.59; Geranylgeranyl transferase type I subunit beta; GGTase-I-beta; Type I protein geranyl-geranyltransferase subunit beta
Species
Mus musculus (Mouse)
Expression Region
1-377
Target Protein Sequence
MATTEDDRLA GSGEGERLDF LRDRHVRFFQ RCLQVLPERY SSLETSRLTI AFFALSGLDM LDSLDVVNKD DIIEWIYSLQ VLPTEDRSNL SRCGFRGSSY LGIPFNPSKN PGAAHPYDSG HIAMTYTGLS CLIILGDDLG RVDKEACLAG LRALQLEDGS FCAVPEGSEN DMRFVYCASC ICYMLNNWSG MDMKKAISYI RRSMSYDNGL AQGAGLESHG GSTFCGIASL CLMGKLEEVF SEKELNRIKR WCIMRQQNGY HGRPNKPVDT CYSFWVGATL KLLKIFQYTN FEKNRNYILS TQDRLVGGFA KWPDSHPDAL HAYFGICGLS LMEESGICKV HPALNVSTRT SERLRDLHQS WKTKDSKQCS DNVHIAT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the transfer of a geranyl-geranyl moiety from geranyl-geranyl pyrophosphate to a cysteine at the fourth position from the C-terminus of proteins having the C-terminal sequence Cys-aliphatic-aliphatic-X. Known substrates include RAC1, RAC2, RAP1A and RAP1B.
Gene References into Functions
  1. The results showed that the magnitude of long-term potentiation (LTP) was markedly suppressed in hippocampal slices from GGT-haplodeficient mice. Consistent with the synaptic dysfunction, there was a significant decrease in cortical spine density and cognitive function in GGT-haplodeficient mice. PMID: 29406266
  2. This study demonstrated that Inhibiting geranylgeranyltransferase I activity decreases spine density in central nervous system. PMID: 25330763
  3. A decrease in membrane-associated Rho proteins in aged brain is associated with down-regulation of GGTase-Ibeta. PMID: 24428713
  4. Targeting GGTase-I activates RHOA and leads to increased macrophage reverse cholesterol transport and reduced atherosclerosis development despite a significant increase in inflammation. PMID: 23334894
  5. Data show that mice lacking GGTase-I in macrophages develop severe joint inflammation resembling erosive rheumatoid arthritis. PMID: 21266780

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Protein Families
Protein prenyltransferase subunit beta family
Database Links
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