Recombinant Mouse Glutamyl aminopeptidase (Enpep), partial

Code CSB-YP007678MO
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Source Yeast
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Code CSB-EP007678MO
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Source E.coli
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Code CSB-EP007678MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007678MO
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Source Baculovirus
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Code CSB-MP007678MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Enpep
Uniprot No.
Alternative Names
EnpepGlutamyl aminopeptidase; EAP; EC 3.4.11.7; Aminopeptidase A; AP-A; BP-1/6C3 antigen; CD antigen CD249
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulates central hypertension through its calcium-modulated preference to cleave N-terminal acidic residues from peptides such as angiotensin II.
Gene References into Functions
  1. de-regulation of both PITX2 and ENPEP could contribute to an increased risk of atrial fibrillation in carriers of disease-associated variants. PMID: 25888893
  2. Demonstrate a novel role of APA in ANG-(1-7) metabolism and suggest that the upregulation of APA, which occurs after myocardial infarction, may deprive the heart of cardioprotective ANG-(1-7). PMID: 24464749
  3. Ischemia-induced angiogenesis is impaired in APA-KO mice partly through decreased HIF-1alpha stability by proteasomal degradation and subsequent suppression of HIF-1alpha-driven target protein expression such as growth factors. PMID: 20946870
  4. dietary fatty acids affect activity in moue testis PMID: 11903661
  5. Replacement of active site residue glutamate-215 of aminopeptidase A with aspartate or alanine leads to a strong decrease in the rate of hydrolysis of the synthetic substrate alpha-L-glutamyl-beta-naphthylamide. PMID: 14674752
  6. the C-terminal domain of aminopeptidase A acts as an intramolecular chaperone PMID: 15263000
  7. aminopeptidase A has a role in angiotensin II conversion to angiotensin III and in water but not alcohol consumption PMID: 16889841
  8. Results demonstrate a key role of Thr-348 in substrate specificity of aminopeptidase A for N-terminal acidic amino acids by insuring the optimal positioning of the substrate during catalysis. PMID: 19228697
  9. Lack of angiotensin II conversion to angiotensin III increases water but not alcohol consumption in aminopeptidase A-deficient mice.Elevated central concentrations of the vasopressor octapeptide angiotensin (Ang) II increase the water intake in mammals. PMID: 16889841

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Subcellular Location
Cell membrane; Single-pass type II membrane protein.
Protein Families
Peptidase M1 family
Tissue Specificity
Early B-lineage cells and certain stromal cell of hemopoietic tissues. Also expressed by capillary endothelial cells, placenta, and epithelial cells of the intestine and proximal renal tubules.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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