Recombinant Mouse Histone H2A.x (H2afx)

1. H2AX has a physiologic function in mediating influences of oxidative stress on NRF2-transcriptional targets and behavior PMID: 29670103
2. 14days of variable stress, but not a single stress exposure, was associated with increased bed nucleus of the stria terminalis levels of gammaH2AX 24h after termination of the stress paradigm. Phosphorylation levels of a pair of kinases associated with the DNA damage response, glycogen synthase kinase 3 beta (GSK3beta) and p38 mitogen-activated protein kinase (MAPK) were also elevated following variable stress. PMID: 29352998
3. The expansion of all three waves of H2AX phosphorylation from the leptotene to pachytene stages is regulated by MOF in meiosis. PMID: 29795555
4. The results propose a model in which Aurora B-mediated H2AX-phosphorylated serine 121 probably provide a platform for Aurora B autoactivation circuitry at centromeres and thus play a pivotal role in proper chromosome segregation. PMID: 27389782
5. TSSK6 is required for gammaH2AX formation during spermiogenesis, and also link gammaH2AX to the histone-to-protamine transition and male fertility. PMID: 28389581
6. Our data show that gammaH2AFX enrichment extends as far as 9-15 Mb of the annotated genomic sequence of the q-arms of the translocated chromosomal trivalents and that both gammaH2AFX and H3.3 levels are reduced over the X chromosome. Our data are also suggestive of an asymmetry in gammaH2AFX and H3.3 enrichment with a bias toward the non-translocated homolog. PMID: 27090237
7. Results suggest activation of H2AX via promoter demethylation in specific populations of basal mammary cells that is induced by a signal from neighboring luminal cells with hyper STAT5 activity. PMID: 27260000
8. The findings demonstrate that RNF168 couples PALB2-dependent homologous recombination to H2A ubiquitylation to promote DNA repair and preserve genome integrity. PMID: 28240985
9. A report on a role of H2AX in non-homologous end joining that repairs a site-specific chromosomal DNA double strand breaks PMID: 28977657
10. The authors observe that persistent accumulation of reactive oxygen species, due to a deficient JunD-/Nrf2-antioxidant response, reduces H2AX protein levels. This effect is mediated by an enhanced interaction of H2AX with the E3 ubiquitin ligase RNF168, which is associated with H2AX poly-ubiquitination and promotes its degradation by the proteasome. PMID: 27006338
11. The findings highlight specific non-overlapping functions of PARP1 and PARP2 at H2AX-deficient chromatin during replicative phases of the cell cycle and uncover a unique requirement for PARP1 in nonhomologous end-joining-deficient cells. PMID: 27373144
12. Unlike directly induced DSBs, secondary DSBs were not efficiently repaired, although Rad51 and 53BP1 were recruited to these sites. H2AX was dramatically stabilized in response to DSBs directly caused by gamma-rays, enabling gammaH2AX foci formation and DSB repair, whereas H2AX was barely stabilized in response to secondary DSBs, in which gammaH2AX foci were small and DSBs were not efficiently repaired PMID: 27251002
13. this study shows that T cells and thyrocytes in a mouse model of thyrocyte hyperplasia has foci of phosphorylated histone protein H2A.X, indicative of cellular senescence PMID: 27173733
14. The role of H2AX phosphorylation and H3K56 acetylation on normal stem cell response to radiation PMID: 26941327
15. H2AX knockout mice showed cell-autonomous anaemia and erythroid dysplasia, mimicking dyserythropoiesis in MDS. PMID: 26791933
16. These data suggest a role for FoxO3a in the maintenance of genome integrity in response to DNA damage that is mediated by H2AX via yet unknown mechanisms. PMID: 26694365
17. overexpression of TIPRL promotes phosphorylation of H2AX, and increases gamma-H2AX positive foci in response to DNA damage, whereas knockdown of TIPRL inhibits gamma-H2AX phosphorylation PMID: 26717153
18. we find that asynapsed supernumerary chromosomes do not elicit prophase I loss, despite being enriched for gH2AFX and other checkpoint proteins PMID: 26509888
19. link gamma-H2AX to transcription, assigning a new function for this DNA damage marker PMID: 26045162
20. DNA double-strand breaks by Cr(VI) are targeted to euchromatin and cause ATR-dependent phosphorylation of histone H2AX and its ubiquitination. PMID: 25288669
21. Exposure to Cr(VI) induced villus blunting and crypt hyperplasia in the duodenum. gamma-H2AX immunostaining was elevated in villi, but not in the crypt compartment. PMID: 25352572
22. Data indicate that crypts exposed to 180 ppm hexavalent chromium Cr(VI) appeared healthy and exhibited no difference in gammaH2ax (gamma-H2AX) staining relative to those of control. PMID: 26232259
23. the gamma-H2AX biomarker showed higher sensitivity to measure dose-rate effects after low-dose LDR X rays compared to MNi formation. PMID: 25738897
24. H2A.X is specifically targeted to and negatively regulates extraembryonic lineage gene expression in embryonic stem cells (ESCs) and prevents trophectoderm lineage differentiation. PMID: 25192463
25. Oxidative DNA damage in postnatal retina increases during development. It is low during the first postnatal week when PARP-1 activity is high but increases thereafter. PMID: 25650421
26. H2ax functions in a haploinsufficient manner to suppress allelic imbalances and limit molecular heterogeneity within and among Emu-c-Myc lymphomas. PMID: 23966158
27. Heat-shock induced gammaH2AX foci are associated with the nuclear matrix only in S-phase cells. PMID: 23824453
28. In the absence of LSH, the histone variant H2AX is not efficiently phosphorylated in response to DNA damage. PMID: 22946062
29. Expression of H2AX and its phosphorylated form is a critical factor that determines drug sensitivity. PMID: 23536184
30. Findings suggest a novel function of H2AX that expands the knowledge of this histone variant beyond its role in DNA damage and into a new specialized biological function in mouse pluripotent stem cells. PMID: 22628289
31. a distinct monoubiquitination-dependent mechanism that is required for H2AX phosphorylation and the initiation of DDR. PMID: 21676867
32. Studies indicate that embryonic fibroblast cells preserve an H2AX diminished quiescent status through p53 regulation and stable-diploidy maintenance. PMID: 21858116
33. study reports orf36 facilitated expression of RTA, an immediate early MHV68 gene and DNA synthesis during infection of primary macrophages; H2AX expression supported efficient RTA transcription and phosphorylated H2AX associated with RTA promoter PMID: 21943826
34. monoubiquitination of H2AX is an important step for DNA damage response PMID: 21690091
35. These data suggest that, at long times after irradiation, mechanisms additional to impairment of DNA break repair may account for the long persistence of gamma-H2AX foci in male germ cells. PMID: 21498842
36. Cell cycle restriction by histone H2AX limits proliferation of adult neural stem cells. PMID: 21436033
37. Germline inactivation of H2ax and p53 gene deletion is asssociated with the development of thymic lymphoma. PMID: 20947684
38. H2AX preserves the structural integrity of broken DNA ends in G1-phase lymphocytes, thereby preventing these DNA ends from accessing repair pathways that promote genomic instability PMID: 21160476
39. The homologous recombination and ionizing radiation-resistance functions of H2AX are controlled in large part by specific MDC1-interacting residues of H2AX, but that additional H2AX residues modulate these core functions of H2AX. PMID: 20703100
40. H2A.X exhibited significant incorporation during the early pre-implantation stage after fertilization; global changes in the composition of histone H2A variants in chromatin play a role in genome remodeling after fertilization PMID: 20943707
41. This work reveals viral kinase-dependent regulation of gammaherpesvirus latency and illuminates a novel link between H2AX, a component of a tumor suppressor DDR network, and in vivo latency of a cancer-associated gammaherpesvirus. PMID: 20557919
42. histone H2A.X phosphorylation by DNA-dependent protein kinase is not affected by core histone acetylation, but it alters nucleosome stability and histone H1 binding PMID: 20356835
43. Data show that H2AX is essential for viability in a PARP1-deficient background, while deficiency for 53BP1 modestly exacerbates phenotypes of growth retardation, genomic instability, and organismal radiosensitivity observed in PARP1-deficient mice. PMID: 20231360
44. Study shows that, through its C-terminal proline-rich binding domain (PBD, residues 543-559), AIF associates in the nucleus with histone H2AX. PMID: 20360685
45. Wild-type p53-induced phosphatase 1 dephosphorylates histone variant gamma-H2AX and suppresses DNA double strand break repair. PMID: 20118229
46. MRE11-RAD50-NBS1 complex dictates DNA repair independent of H2AX. PMID: 19910469
47. critical for facilitating the assembly of specific DNA-repair complexes on damaged DNA PMID: 11934988
48. absence causes increasd ionizing radiation sensitivity and genomic instability PMID: 12034884
49. Function for gamma-H2AX during meiosis. Function of H2AX phosphorylation during spermatogenesis is restricted to formation of gamma-H2AX foci at DNA double-strand breaks. PMID: 12533428
50. Data show that histone H2AX is not required for somatic hypermutation (SHM), suggesting that the processing of DNA lesions leading to SHM is fundamentally different from class switch recombination. PMID: 12810694

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KEGG: mmu:15270

STRING: 10090.ENSMUSP00000051432

UniGene: Mm.245931