Product Details

Purity

>85% (SDS-PAGE)

Target Names

Iigp1

Uniprot No.

Q9QZ85

Alternative Names

Iigp1; Irga6; Interferon-inducible GTPase 1; EC 3.6.5.-

Species

Mus musculus (Mouse)

Expression Region

2-413

Target Protein Sequence

GQLFSSPKS DENNDLPSSF TGYFKKFNTG RKIISQEILN LIELRMRKGN IQLTNSAISD ALKEIDSSVL NVAVTGETGS GKSSFINTLR GIGNEEEGAA KTGVVEVTME RHPYKHPNIP NVVFWDLPGI GSTNFPPNTY LEKMKFYEYD FFIIISATRF KKNDIDIAKA ISMMKKEFYF VRTKVDSDIT NEADGKPQTF DKEKVLQDIR LNCVNTFREN GIAEPPIFLL SNKNVCHYDF PVLMDKLISD LPIYKRHNFM VSLPNITDSV IEKKRQFLKQ RIWLEGFAAD LVNIIPSLTF LLDSDLETLK KSMKFYRTVF GVDETSLQRL ARDWEIEVDQ VEAMIKSPAV FKPTDEETIQ ERLSRYIQEF CLANGYLLPK NSFLKEIFYL KYYFLDMVTE DAKTLLKEIC LRN

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

GTPase with low activity. Has higher affinity for GDP than for GTP. Plays a role in resistance to intracellular pathogens. Required for disruption of the parasitophorous vacuole formed following T.gondii infection and subsequent killing of the parasite. Mediates resistance to C.trachomatis infection by targeting bacterial inclusions to autophagosomes for subsequent lysosomal destruction.

Gene References into Functions

The study determined the crystal structure of an oligomerization-impaired Irga6 mutant bound to a non-hydrolyzable GTP analog. PMID: 26934976
These data identify Toxoplasma gondii GRA7 as a regulator for ROP18-specific inactivation of murine Irga6. PMID: 26247512
ROP5 uses a highly polymorphic surface to bind adjacent to the nucleotide-binding domain of an IRG and that this produces a profound allosteric change in the IRGa6 structure. [Rop5] PMID: 25118287
This study defines the catalytic G-domain interface by mutagenesis and presents a model of how GTP hydrolysis is activated in Irga6 complexes, based on the substrate-twinning reaction mechanism of the signal recognition particle and its receptor (SRalpha). PMID: 21276251
Toxoplasma gondii ROP5 binding alters the conformation of the Irga6 active site. PMID: 22802726
results implicate Irga6 in the control of infection with T. gondii and further highlight the importance of the IRG system for resistance to this pathogen PMID: 21698150
Virulent T. gondii strain ROP18 kinase phosphorylates host Irga6 at two threonines in the nucleotide-binding domain, biochemically inactivating the GTPase and inhibiting its accumulation and action at the T. gondii parasitophorous vacuole membrane. PMID: 21203588
role of constitutive hepatocyte expression of Irga6 in resistance to parasites invading from the gut via the hepatic portal system PMID: 20368812
study clearly adds interferon inducible GTPase(IIGP1) to the small group of cooperative GTPase families that appear to characterize the development of intracellular resistance during the interferon response to infection PMID: 12732635
Identified a microtubule binding protein hook3 as its interacting protein. PMID: 15075236
IIGP1 is associated with the endoplasmic reticulum by an N-terminal myristoylation modification PMID: 15294976
Here we present the first crystal structure of a member of the p47 GTPase family, IIGP1, in its nucleotide-free, GDP-, and GppNHp-bound form. Sequence comparison and secondary structure prediction suggest it is a valid model for the p47 GTPase family PMID: 15350217
Differential induction by various gram-negative lipopolysaccharides was myeloid differentiation factor 88 (MyD88)-independent PMID: 16469053

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Subcellular Location

Cytoplasm. Nucleus membrane; Peripheral membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. Parasitophorous vacuole membrane. Note=Localizes to the bacterial inclusions formed following C.trachomatis infection. Accumulates in a GTP-bound form on the parasitophorous vacuole membranes formed following T.gondii infection but exists in a GDP-bound form in uninfected cells.

Protein Families

TRAFAC class dynamin-like GTPase superfamily, IRG family

Database Links

KEGG: mmu:60440

STRING: 10090.ENSMUSP00000032473

UniGene: Mm.261140

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Recombinant Mouse Interferon-inducible GTPase 1 (Iigp1)

Product Details

Customer Reviews and Q&A

Target Background

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