Recombinant Mouse Laminin subunit gamma-3 (Lamc3), partial

Code CSB-YP865625MO
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Source Yeast
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Code CSB-EP865625MO
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Source E.coli
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Code CSB-EP865625MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP865625MO
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Source Baculovirus
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Code CSB-MP865625MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lamc3
Uniprot No.
Alternative Names
Lamc3; LAMC3_MOUSE; Laminin subunit gamma-3; Laminin-12 subunit gamma; Laminin-14 subunit gamma; Laminin-15 subunit gamma
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.
Gene References into Functions
  1. Deletion of Lamb2 and Lamc3 genes affects astrocyte migration and spatial patterning in the retina. PMID: 23571221
  2. gamma3-containing laminins are expressed in a wide variety of basement membranes. gamma3 is expressed in CNS but not peripheral vascular basement membranes. PMID: 22222602
  3. a strong impact of laminin beta2 and gamma3 subunits on the expression and function of both aquaporin-4 and Kir4.1, two important membrane proteins in Muller cells. PMID: 21283711
  4. In human but not mouse fetal brain, LAMC3 is enriched in postmitotic cortical plate neurons, localizing primarily to the somatodendritic compartment. PMID: 21572413
  5. Laminin gamma3 chain, located in murine basement membranes, binds to nidogen PMID: 15824114
  6. netrin-4 is a component of basement membranes and is integrated into the laminin polymer via interactions with the laminin gamma1 andgamma3 short arms PMID: 17588941

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Subcellular Location
Secreted, extracellular space, extracellular matrix, basement membrane.
Tissue Specificity
Strongly expressed in capillaries and arterioles of kidney as well as in interstitial Leydig cells of testis.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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