Recombinant Mouse Latexin (Lxn)

Code CSB-YP013240MO
MSDS
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Source Yeast
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Code CSB-EP013240MO
MSDS
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Source E.coli
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Code CSB-EP013240MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013240MO
MSDS
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Source Baculovirus
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Code CSB-MP013240MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lxn
Uniprot No.
Alternative Names
LxnLatexin; Endogenous carboxypeptidase inhibitor; ECI; Tissue carboxypeptidase inhibitor; TCI
Species
Mus musculus (Mouse)
Expression Region
1-222
Target Protein Sequence
MEIPPTHYAA SRAASVAENC INYQQGTPHK LFLVQTVQQA SKEDIPGRGH KYHLKFSVEE IIQKQVTVNC TAEVLYPQMG QGSAPEVNFT FEGEIGKNPD EEDNTFYQSL MSLKRPLEAQ DIPDNFGNVS PQMKPVQHLA WVACGYVMWQ NSTEDTWYKM LKIQTVKQVQ RNDDFIELDY TILLHDIASQ EIIPWQMQVL WHPQYGTKVK HNSRLPKEGQ AE
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. May play a role in inflammation.
Gene References into Functions
  1. By using an Lxn knockout mouse model, this study found that Lxn inactivation in vivo led to the physiological expansion of the entire hematopoietic hierarchy. Loss of Lxn enhanced the competitive repopulation capacity and survival of hematopoietic stem cells in a cell-intrinsic manner. PMID: 28330618
  2. Rps3 knockdown increased the radiation sensitivity of FDC-P1, confirming that the mechanism of action of Lxn is mediated by Rps3 pathway. PMID: 25341047
  3. These results are consistent with a tumor suppressor role for latexin. PMID: 23028717
  4. Latexin is preferentially expressed in hematopoietic stem cells, and is co-localized with the molecules responsible for the interaction of hematopoietic stem cells with a bone marrow niche. PMID: 21567403
  5. Data indicate that AAV-mediated latexin transduction or administration of a small molecule carboxypeptidase A inhibitor significantly reduced acetone-evoked nociceptive behavior after SNI. PMID: 21572518
  6. The crystal structure of mouse latexin, solved at 1.83 A resolution, shows no structural relationship with other carboxypeptidase inhibitors. PMID: 15698574
  7. Latexin is involved in the transmission of pain, since mice lacking this protein experience reducted pain sensitivity. PMID: 16469302
  8. promoter polymorphisms between the B6 and D2 alleles may affect Lxn gene expression and consequently influence the population size of hematopoietic stem cells. PMID: 17220891
  9. These results indicate that latexin is involved in BMP-2-induced chondrocyte differentiation and plays an important role in skeletogenesis and skeletal regeneration. PMID: 19059214

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Subcellular Location
Cytoplasm.
Protein Families
Protease inhibitor I47 (latexin) family
Tissue Specificity
Highly enriched in macrophages.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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