Recombinant Mouse Lysine-specific demethylase 4A (Kdm4a), partial

Code CSB-YP804868MO
MSDS
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Source Yeast
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Code CSB-EP804868MO
MSDS
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Source E.coli
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Code CSB-EP804868MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP804868MO
MSDS
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Source Baculovirus
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Code CSB-MP804868MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Kdm4a
Uniprot No.
Alternative Names
Kdm4a; Jhdm3a; Jmjd2; Jmjd2a; Kiaa0677Lysine-specific demethylase 4A; EC 1.14.11.-; JmjC domain-containing histone demethylation protein 3A; Jumonji domain-containing protein 2A
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
Gene References into Functions
  1. Jmjd2 class demethylases are critical epigenetic regulators involved in etiopathology of depression and related disorders. PMID: 27711046
  2. High Jmjd2a expression is associated with neuropathic pain. PMID: 29054488
  3. This study dissects an important dual role for maternal Kdm4a in determining faithful early embryonic development and the implantation process. PMID: 28827393
  4. We propose the inhibition of KDM4A activity as a strategy to suppress IL-6 production and attenuate colitis induction. PMID: 28511912
  5. these data reveal a JMJD2A/ETV1/YAP1 axis that promotes prostate cancer initiation and that may be a suitable target for therapeutic inhibition. PMID: 26731476
  6. As well as three novel motifs in JMJD2A-regulated genes. PMID: 25193078
  7. Overexpression of KDM4 proteins induces numerous oncogenic effects. (Review) PMID: 24219278
  8. JMJD2A bound to the FHL1 promoter in response to TAC, upregulated FHL1 expression, and downregulated H3K9 trimethylation. JMJD2A promotes cardiac hypertrophy under pathological conditions by a novel mechanism. PMID: 21555854
  9. JHDM3A(GFP)(701) is a suitable catalytic module that can be targeted, under the control of a guide protein, to specific loci where the chromatin H3K9me3 status and the milieu of gene expression are to be modified. PMID: 21148561

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Subcellular Location
Nucleus.
Protein Families
JHDM3 histone demethylase family
Tissue Specificity
Widely expressed.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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