Recombinant Mouse Mothers against decapentaplegic homolog 9 (Smad9)

Code CSB-YP884919MO
MSDS
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Source Yeast
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Code CSB-EP884919MO
MSDS
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Source E.coli
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Code CSB-EP884919MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP884919MO
MSDS
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Source Baculovirus
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Code CSB-MP884919MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Smad9
Uniprot No.
Alternative Names
Smad9; Madh8; Madh9; Smad8; Mothers against decapentaplegic homolog 9; MAD homolog 9; Mothers against DPP homolog 9; SMAD family member 9; SMAD 9; Smad9; Smad8
Species
Mus musculus (Mouse)
Expression Region
1-430
Target Protein Sequence
MHPSTPISSL FSFTSPAVKR LLGWKQGDEE EKWAEKAVDS LVKKLKKKKG AMDELERALS CPGQPSKCVT IPRSLDGRLQ VSHRKGLPHV IYCRVWRWPD LQSHHELKPL ECCEFPFGSK QKEVCINPYH YRRVETPVLP PVLVPRHSEY NPQLSLLAKF RSASLHSEPL MPHNATYPDS FQQSLCPAPP SSPGHVFPQS PCPTSYPHSP GSPSESDSPY QHSDFRPVCY EEPQHWCSVA YYELNNRVGE TFQASSRSVL IDGFTDPSNN RNRFCLGLLS NVNRNSTIEN TRRHIGKGVH LYYVGGEVYA ECVSDSSIFV QSRNCNYQHG FHPATVCKIP SGCSLKVFNN QLFAQLLAQS VHHGFEVVYE LTKMCTIRMS FVKGWGAEYH RQDVTSTPCW IEIHLHGPLQ WLDKVLTQMG SPHNPISSVS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). Has been shown to be activated by activin type I receptor-like kinases (ALK-2, ALK-3, ALK-6) which stimulate heteromerization between SMAD9 and SMAD4. May play a role in osteoblast differentiation and maturation.
Gene References into Functions
  1. Sphingosine 1 phosphate also up-regulated runt-related transcription factor 2 (Runx2) expression through S1PR2/RhoA/ROCK/Smad1/5/8 signaling. PMID: 27612439
  2. Phosphorylated Smad1/5/8/9 specifically bound to the BREs of Smad8/9 gene. The present study reveals that Smad8/9 is a unique R-Smad regulated through the BMP pathway at the mRNA level. PMID: 26748560
  3. findings suggest that Smad9 is a new type of transcriptional regulator in BMP signaling. PMID: 25534700
  4. The findings demonstrate for the first time that KMUP-1 can promote osteoblast maturation and differentiation in vitro via BMP-2/Smad1/5/8 and Wnt/beta-catenin pathways. PMID: 25536014
  5. Data indicate that PDGF-AA promotes mesenchymal stem cel migration via bone morphogenetic protein 2 (BMP2)-smad proteins smad1/5/8 activation requires lysosome-mediated degradation of PDGF alpha Receptor (PDGFRalpha). PMID: 25470749
  6. our data suggest a pivotal role for canonical BMP signaling demonstrating distinguished nonoverlapping function of pSmad8 with pSmad1 and pSmad5 in hfSCs regulation and hair morphogenesis but a redundant role in adult hair progenitors differentiation. PMID: 24023003
  7. FGF2 inhibited BMP9-induced osteogenic differentiation by blocking BMP9-induced Smads signaling and subsequently reducing Smads dependent up-regulation of ALK1 and ALK2 in mesenchymal stem cells. PMID: 23680673
  8. a detailed computational model for TGF-beta signalling that incorporates elements of previous models together with crosstalking between Smad1/5/8 and Smad2/3 channels through a negative feedback loop dependent on Smad7. PMID: 23804438
  9. BMP4-mediated signaling in adult mouse ovary-derived oogonial stem cells cultured in vitro drives differentiation of these cells into oocytes through Smad1/5/8 activation and transcriptional up-regulation of key meiosis-initiating genes. PMID: 23993924
  10. Endogenous FGF2 is necessary for BMP-2 induced nuclear accumulation and co-localization of Runx-2 and phospho-Smads1/5/8. PMID: 23559326
  11. Smad8/BMP2-engineered mesenchymal stem cells induce accelerated recovery of the biomechanical properties of the Achilles tendon PMID: 22696396
  12. Dynamic regulation of Smad expression during mesenchyme to epithelium transition in the metanephric kidney. PMID: 11850197
  13. Smad8 has critical function in the development of the mammalian central nervous system. PMID: 15899870
  14. results suggest that BMP2/BMP4 signalling through SMAD 8 is required for transcriptional activation of the mouse Msx1 gene PMID: 16101586
  15. we demonstrate that BMP-2, -5, and -7 stimulate receptor-activated Smad1, 5, and 8, which in turn causes oligomerization of Smad4 in the nucleus. PMID: 16522160
  16. Initially expressed only in the visceral yolk sac endoderm and shows a highly restricted pattern of expression in the embryo proper at later stages. PMID: 16765933
  17. Data show that Smad8 expression is ubiquitous during testis development but becomes cell-specific in the adult. PMID: 18069690
  18. Smad8 loss of function resulted in changes in pulmonary arteries including medial thickening and smooth muscle hyperplasia observed in patients with pulmonary artery hypertension. PMID: 19419974

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Subcellular Location
Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Protein Families
Dwarfin/SMAD family
Database Links
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