Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

Madcam1

Uniprot No.

Q61826

Research Area

Immunology

Alternative Names

Madcam1; Mucosal addressin cell adhesion molecule 1; MAdCAM-1; mMAdCAM-1

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

22-364aa

Target Protein Sequence

QSFQVNPPESEVAVAMGTSLQITCSMSCDEGVARVHWRGLDTSLGSVQTLPGSSILSVRGMLSDTGTPVCVGSCGSRSFQHSVKILVYAFPDQLVVSPEFLVPGQDQVVSCTAHNIWPADPNSLSFALLLGEQRLEGAQALEPEQEEEIQEAEGTPLFRMTQRWRLPSLGTPAPPALHCQVTMQLPKLVLTHRKEIPVLQSQTSPKPPNTTSAEPYILTSSSTAEAVSTGLNITTLPSAPPYPKLSPRTLSSEGPCRPKIHQDLEAGWELLCEASCGPGVTVRWTLAPGDLATYHKREAGAQAWLSVLPPGPMVEGWFQCRQDPGGEVTNLYVPGQVTPNSSS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

52.8kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both the integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Both isoform 1 and isoform 2 can adhere to integrin alpha-4/beta-7. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

Gene References into Functions

Nkx2-3 deficiency reprograms the endothelial addressin preference for lymphocyte homing in Peyer's patches. PMID: 25320278
findings indicate that alpha4beta7*IgG can be used as a probe for functional MAdCAM-1 expressed on HEVs in GALT and could potentially serve as an anti-inflammatory drug inhibiting GALT-specific lymphocyte migration PMID: 21430257
ectopic expression of MAdCAM-1 at the BBB does not increase alpha4beta7-integrin-mediated immune cell trafficking into the CNS during MOG(aa35-55)-induced experimental autoimmune encephalomyelitis PMID: 21341265
Observed a significant decrease in expression of ICAM-1 and MAdCAM-1 mRNA/protein in lactobacillus plantarum treated colitis. PMID: 19960256
results suggest that AT1R regulates the expression of MAdCAM-1 under colonic inflammatory conditions through regulation of the translocation of NF-kappaB into the nucleus PMID: 19940029
Lymphocyte binding to MAdCAM-1 via alpha4beta7 integrin activates a signal transduction pathway involving tyrosine phosphorylation of paxillin and p105(Cas-L) PMID: 11947929
Mucosal addressin cell adhesion molecule 1 plays an unexpected role in the development of mouse guard hair. PMID: 12230506
spatial heterogeneity of MAdCAM-1 and VCAM-1 activation following TNF-alpha challenge may promote specific T lymphocyte recruitment in the inflamed colonic mucosa PMID: 12388196
Increased expression of mucosal addressin cell adhesion molecule-1 in gastritis induced by Helicobacter pylori PMID: 12390304
expression of MAdCAM-1 by fibroblasts and melanomas suggests MAdCAM-1 may play a role in regulating host responses in the periphery, leucocyte transmigration across nonendothelial boundaries, or the homotypic interactions of some malignant melanomas PMID: 12848854
GST-3 is responsible for MECA-79 (MADCAM1)defined luminal ligands PMID: 15111310
MAdCAM-1 plays important roles in T-lymphocyte adherence in all intestinal sites. PMID: 15371130
In a chronic ileitis model, pathogenic CD4+ T cells use the MAdCAM-1/alpha4beta7 physiologic pathway in order to recirculate to the chronically inflamed small intestine. PMID: 15699171
In wild-type mice administered blocking monoclonal antibody (mAb) to VCAM1-1 but nor to Madcam1 recruitment of mast cells to the inflamed lung was not reduced. PMID: 16670268
These data indicate that TNF-alpha deficiency alters homoeostasis of the colonic chemokine/cytokine environment and humoral immune response, resulting in an exacerbation of acute DSS-induced colitis in TNF-alpha(-/-) mice. PMID: 17768420
Combined effects of diabetes and pregnancy on pancreatic and decidual expression of VCAM-1, MAdCAM-1 and PNAd. PMID: 18031809
Expression of MAdCAM-1 in isolated lymphoid follicles (ILFs) of the small intestine colocalizes with CD31 expression, indicating that MAdCAM-1 expression in ILFs is restricted to vascular structures. PMID: 18768861

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Involvement in disease

Absence of Madcam1 in the spleen has been found in aly/aly mice, but normal expression is found in intestinal venules. This aberrant expression is a secondary defect and not the direct cause of aly alymphoplasia, an autosomal recessive mutation which induces total aplasia of lymph nodes and Peyer patches.

Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Highly expressed on high endothelial venules (HEV) of organized intestinal lymphoid tissues like the Peyer patches and mesenteric lymph nodes, and in the lamina propria of the intestine. Some expression found in the spleen, and low levels of expression in

Database Links

STRING: 10090.ENSMUSP00000020554

UniGene: Mm.391556

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Code	CSB-EP717229MO
MSDS	MSDS
Size	US$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Mucosal addressin cell adhesion molecule 1 (Madcam1), partial

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