Recombinant Mouse Neurogenin-2(Neurog2)

Code CSB-YP015731MO
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Source Yeast
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Code CSB-EP015731MO
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Source E.coli
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Code CSB-EP015731MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP015731MO
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Source Baculovirus
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Code CSB-MP015731MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Neurog2
Uniprot No. P70447
Alternative Names Neurog2; Ath4a; Atoh4; Ngn2; Neurogenin-2; NGN-2; Helix-loop-helix protein mATH-4A; mATH4A; Protein atonal homolog 4
Species Mus musculus (Mouse)
Expression Region 1-263
Target Protein Sequence MFVKSETLEL KEEEEVLMLL GSASPASATL TPMSSSADEE EDEELRRPGS ARGQRGAEAE QGVQGSPASG AGGCRPGRLL GLMHECKRRP SRSRAVSRGA KTAETVQRIK KTRRLKANNR ERNRMHNLNA ALDALREVLP TFPEDAKLTK IETLRFAHNY IWALTETLRL ADHCAGAGGL QGALFTEAVL LSPGAALGAS GDSPSPPSSW SCTNSPASSS NSTSPYSCTL SPASPGSDVD YWQPPPPEKH RYAPHLPLAR DCI
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Transcriptional regulator. Involved in neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3').
Gene References into Functions
  1. data support the hypothesis that Neurog2 acts at the top of a retinal bHLH transcription factor hierarchy. Neurog2 simultaneously promotes terminal cell cycle exit and retinal ganglion cell differentiation. PMID: 29770538
  2. Authors conclude that the Neurog2 gene is expressed and active prior to birth, but also exerts an influence on postnatal retinal neuron differentiation. PMID: 30048641
  3. Neurog2 and Ascl1 altered the expression of Ikaros and Foxg1. PMID: 28584103
  4. data suggest that common reprogramming mechanisms exist driving the conversion of lineage-distant somatic cell types to neurons and reveal a critical role for CEND1 in NEUROG2-driven astrocytic reprogramming. PMID: 26321141
  5. Findings suggest the strict spatio-temporal expression of Notch effector genes plays an important role during hippocampal neurogenesis and suggests that Notch1, Hes1 and Ngn2 were regulated by changing some specific CpG sites of their promoters. PMID: 25701255
  6. IFN-gamma inhibits neuronal differentiation of neural progenitor cells by negatively regulating the expression of Neurog2 via the JAK/STAT1 pathway. PMID: 26325468
  7. In mid-embryonic neocortical walls, expression of Neurog2-d4Venus mostly overlapped with that of Neurog2 protein, with a slightly (1 h) delayed onset. PMID: 24712911
  8. APP-stimulated neuronal differentiation of NSPCs is mediated by Ngn2 PMID: 25217641
  9. Notch signaling controls the overall tempo of retinogenesis through Atoh7 and Neurog2, by integrating cell fate specification, the wave of neurogenesis and the developmental status of cells ahead of this wave PMID: 25100656
  10. Neurog1 and Neurog2 have unique and redundant functions in the piriform cortex, controlling the timing of differentiation of neurons PMID: 24403153
  11. Neurog2 functions as a transcriptional activator to initiate the expression of several genetic off-switches, each of which repress distinct gene expression programs during neocortical development. PMID: 22735158
  12. Sustained vs. oscillating expressions of Ngn2, Dll1 and Hes1: a model of neural differentiation of embryonic telencephalon. PMID: 23499991
  13. Neurog1/2 together coordinate development of the olfactory system, which depends on tissue interactions between the olfactory bulb and epithelium. PMID: 22906231
  14. Gene expression analyses revealed that Ngn2-induced in vitro differentiation partially resembled neurogenesis in vivo, as it included specific activation of Ngn2 target genes and interaction partners PMID: 22719915
  15. Neurogenin 1 expression in mouse embryonic stem (ES) cells promotes neural differentiation and inhibits proliferation, thus establishing an approach to graft ES cells safely into the spinal cord. PMID: 22245661
  16. Loss of microprocessor activity results not only in an increase Ngn2 protein but also extended expression of Neurog2 into later stages of the neurogenic lineage. PMID: 22706270
  17. It was shown that, in the cerebellar primordium, neurogenin 2 is expressed by cycling progenitors fated to become Purkinje cells. During cerebellar development, neurogenin 2 is expressed in G1 phase by progenitors poised to exit the cell cycle. PMID: 22669821
  18. temporal regulation of neurogenin 2 heterodimerization by GSK3 is a central component of the neuronal differentiation "clock" that coordinates the timing and tempo of neocortical neurogenesis in mouse PMID: 22674256
  19. the formation of cranial ganglia along the anterior-posterior axis is dependent on the dynamic spatiotemporal activities of Neurog1 and/or Neurog2 in both neural crest and epibranchial placode. PMID: 22102600
  20. Data show that Bmp7 regulates Ngn2 steady-state transcript levels in the E14.5 cortex. PMID: 22461901
  21. Ngn2 is required for the coordinated specification of cardinal features defining the phenotype of cortical pyramidal neurons, including their migration properties, dendritic morphology and axonal projection. PMID: 21864333
  22. NeuroD promoter is substantially more sensitive to the phosphorylation status of Ngn2 than the Delta promoter, and that this can be attributed to differences in the ease of promoter activation. PMID: 22491944
  23. multi-site cdk-dependent phosphorylation of Ngn2 interprets cdk levels to control neuronal differentiation in response to cell cycle lengthening during development PMID: 21852393
  24. Results indicate that LMO4 promotes the acquisition of cortical neuronal identities by forming a complex with NGN2 and subsequently activating NGN2-dependent gene expression. PMID: 21652654
  25. all subtypes of glutamatergic neurons derive from Neurog2 positive progenitors during development of the olfactory bulb PMID: 21466690
  26. Neurog2 controls the leading edge of neurogenesis in the mouse retina. PMID: 20144606
  27. localization of neurogenin2, NeuroD and Mash 1 in the embryonic forebrain is highly similar to the situation described in Xenopus and zebrafish, suggesting a temporally and spatially defined control of neurogenesis during development PMID: 20058311
  28. Transient expression of the bHLH factor neurogenin-2 marks a subpopulation of neural crest cells biased for a sensory but not a neuronal fate. PMID: 12060754
  29. neurogenin 2 developmental expression is regulated by Pax-6 PMID: 12783797
  30. Neurogenin2 determines the projection of thalamic neurons to specific cortical domains by specifying the responsiveness of their axons to cues encountered in an intermediate target, the ventral telencephalon. PMID: 12895419
  31. Sequential phases of cortical specification involve ngn2-dependent and -independent pathways. PMID: 15229646
  32. identified several new components of the neocortex differentiation cascade(s) activated downstream of Ngn1 and Ngn2 PMID: 15328020
  33. Neuron pairs showed uneven/asymmetric expression of NGN2 whereas other pairs were either symmetrically positive or negative. PMID: 15523634
  34. Olig2/Ngn2 ratio in motor neuron progenitor cells serves as a gate for timing proper gene expression during the development. PMID: 15655114
  35. In the absence of Ngn2, there is an increase in the number of dorsal neuronal populations dI3 and dI5 neurons, in contrast to the effects produced by activity of achaete-scute complex homolog-like 1 PMID: 15901662
  36. Ngn2 coordinates the acquisition of the radial migration properties and the unipolar dendritic morphology characterizing pyramidal neurons through molecular mechanisms distinct from those mediating its proneural activity. PMID: 16202708
  37. These data indicate that retinal neurons expressing ngn2 at some point in their life histories may later differentiate into a variety of cell types. PMID: 16364654
  38. Data show that lack of neurogenin (Ngn)2 impairs the development of mesencephalic dopaminergic (mesDA) neurons, such that less than half of the normal mesDA neuron number remain in Ngn2 mutant mice at postnatal stages. PMID: 16396906
  39. These results demonstrate that neurogenin 2 is uniquely required for the development of midbrain dopaminergic neurons. PMID: 16410412
  40. BMP and FGF-2 act as positive and negative regulators in ngn-2 expression, respectively; these signaling molecules regulate the differentiation of sensory neurons and glia. PMID: 16425218
  41. Over-expression of Nurr1 and Ngn2 in combination generates morphologically mature TH-expressing neurons that also express additional mesencephalic markers. PMID: 17291494
  42. Ngn2 transient expression in proliferating neuronal progenitors supports the idea that it plays a significant role in adult neurogenesis. PMID: 17466019
  43. Helt determines GABAergic over glutamatergic fate, by repressing Ngn genes; Helt and Ngns are commonly used in the mesencephalon for determination of the GABAergic versus glutamatergic transmitter phenotype. PMID: 17611227
  44. The proneural gene Ngn2 instructs a partial cortical identity when misexpressed in ventral telencephalic progenitors. PMID: 18160702
  45. Rat and mouse species-dependent differences of Nurr1 and Ngn2 actions in dopamine neuron differentiation. PMID: 18242186
  46. Pax3 regulates Hairy and enhancer of split homolog-1 (Hes1) and Neurogenin2 (Ngn2) by directly binding to their promoters. PMID: 18308300
  47. Novel targets of Ngn2 were identified, such as members of the Notch and Wnt pathways, and proteins involved in adhesion and signal transduction. PMID: 18377642
  48. The phoshorylation of neurogenin 2 at S231 and S234 facilitates the interaction of neurogenin 2 with homeodomain transcription factors to specify motor neuron identity. PMID: 18400164
  49. Data establish a unique role of Ngn2 in DG neurogenesis during development and raise the possibility that Ngn2 has a similar function in adult neurogenesis. PMID: 18448566
  50. Ngn1, Ngn2 and Mash1 are expressed at relevant stages of cerebellar neurogenesis in the prospective cerebellar nuclei and in the ventricular zone PMID: 18498101
  51. Combinatorial signalling controls Combinatorial signalling controls Neurogenin2 expression at the onset of spinal neurogenesis. PMID: 18590718
  52. Results suggest that Notch signaling in nascent daughter cells suppresses a Ngn2-Tbr2 cascade. PMID: 19059340
  53. Taken together, our data illustrate that Ngn2, neuroD and Tbr transcription factors are involved in postnatal neurogenesis in the olfactory bulb. PMID: 19200230
  54. critical role of for Ngn2 and NeuroD1 in controlling neuronal commitment and hippocampal granule neuroblast formation, both during embryonic development and in post-natal hippocampal granule neurogenesis PMID: 19274100
  55. Ngn2 and Mash1 are differentially sensitive to negative regulation by gliogenic factors or a gliogenic substrate PMID: 19350686
  56. novel Ascl1 containing DNA binding complexes interact with specific E-box sites within the Dll3-promoter in vitro. These complexes include Ascl1/Ascl1 homodimers and Ascl1/Neurog2 heterodimers. PMID: 19389376
  57. Data show that Neurog2 expression is directly regulated by the PTF1-Rbpj complex, identifying Neurog2 as the first neural target of Ptf1a and revealing a bHLH cascade in the specification of GABAergic neurons in the dorsal spinal cord and cerebellum. PMID: 19641016

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Subcellular Location Nucleus
Database Links

KEGG: mmu:11924

STRING: 10090.ENSMUSP00000029587

UniGene: Mm.42017

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