Recombinant Mouse POU domain class 2-associating factor 1 (Pou2af1)

Code CSB-YP723755MO
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Source Yeast
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Code CSB-EP723755MO
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Source E.coli
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Code CSB-EP723755MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP723755MO
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Source Baculovirus
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Code CSB-MP723755MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pou2af1
Uniprot No.
Alternative Names
Pou2af1; Obf-1; POU domain class 2-associating factor 1; B-cell-specific coactivator OBF-1; BOB-1; BOB1; OCA-B; OCT-binding factor 1
Species
Mus musculus (Mouse)
Expression Region
1-256
Target Protein Sequence
MLWQKSTAPE QAPAPPRPYQ GVRVKEPVKE LLRRKRGHTS VGAAGPPTAV VLPHQPLATY STVGPSCLDM EVSASTVTEE GTLCAGWLSQ PAPATLQPLA PWTPYTEYVS HEAVSCPYST DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTPRSTATPA VGPQLEGPEH QAPLTYFPWP QPLSTLPTSS LQYQPPAPTL SGPQFVQLPI SIPEPVLQDM DDPRRAISSL TIDKLLLEEE ESNTYELNHT LSVEGF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator.
Gene References into Functions
  1. The number of follicular B2 lymphocytes and expression of the B-cell-specific transcriptional coactivator OcaB increase with age in spleen and in intra-abdominal epididymal white adipose tissue (eWAT), concomitantly with higher circulating levels of IgG and impaired glucose homeostasis. PMID: 29496744
  2. Bob1 promotes the development of follicular T helper cells via Bcl6.Bob1 is directly involved in follicular T helper cell differentiation and required for mounting normal T cell-dependent B-cell responses. PMID: 26957522
  3. Findings indicate octamer transcription factor-1 (Oct1) and Oct binding factor 1 protein (OCA-B) as central mediators of CD4(+) T cell memory. PMID: 26481684
  4. Loss of Obf1 is associated with IgM-mediated glomerulonephritis. PMID: 24217807
  5. The transcription factor Bob1 counteracts B cell activation and regulates miR-146a in B cells. PMID: 24719463
  6. Expressing a stable Bob1 mutant in B cells suppresses cell proliferation and induces changes in surface marker expression commonly seen during B-cell differentiation. PMID: 24061476
  7. OcaB is essential for V(D)J recombination of a subset of Igkappa genes. We show that OcaB modulates recombination by directly enhancing Igkappa gene transcription in vivo. PMID: 12230975
  8. Data show that the expression of BOB.1/OBF.1 in peripheral B cells is required for the development of marginal zone B lymphocytes. PMID: 12417733
  9. Transcriptional coactivator OBF-1 is essential for germinal center formation in Aiolos-deficient mice; lack of OBF-1 reverses several Aiolos mutant mouse phenotypes. PMID: 12574333
  10. B cell-specific transgenic expression of Bcl2 rescues early B lymphopoiesis but not B cell responses in BOB.1/OBF.1-deficient mice PMID: 12732662
  11. OCA-B is involved in B cell expansion as well as B cell function. PMID: 12857960
  12. it was concluded OcaB is required for normal selection of the antibody repertoire in developing B cells PMID: 12917262
  13. BOB.1/OBF.1 has a widespread effect on B cell development and function PMID: 12947107
  14. OBF-1 determines the cell-autonomous capacity of B cells to undergo terminal differentiation in response to different immunological signals. PMID: 15867091
  15. Results suggest that OCA-B is required for pre-BCR and BCR signaling at multiple stages of B cell development through its nontranscriptional regulation of SYK. PMID: 16713566
  16. The present findings establish a hierarchy between these two factors and provide a molecular link between OBF-1 and B cell receptor signaling. PMID: 16861304
  17. BOB.1/OBF.1 regulates the balance of TH1 versus TH2 mediated immunity PMID: 17568779
  18. These results identify OBF-1 as a critical gene in the development of autoantibodies and reveal an essential role for OBF-1 in the generation of antibody/autoantibody-secreting cells in vivo. PMID: 17574818
  19. A novel role for OBF-1 and Aiolos in controlling the transcription and nuclear organization of genes involved in pre-BCR function. PMID: 18974788
  20. Tight regulation of OBF1 expression in early B cells is essential to allow efficient B lymphocyte differentiation. PMID: 19104664

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Subcellular Location
Nucleus.
Protein Families
POU2AF1 family
Tissue Specificity
B-cell specific.
Database Links

KEGG: mmu:18985

STRING: 10090.ENSMUSP00000034554

UniGene: Mm.897

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