Recombinant Mouse Phosphoinositide 3-kinase adapter protein 1 (Pik3ap1)

Code CSB-YP863642MO
MSDS
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Source Yeast
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Code CSB-EP863642MO
MSDS
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Source E.coli
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Code CSB-EP863642MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863642MO
MSDS
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Source Baculovirus
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Code CSB-MP863642MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pik3ap1
Uniprot No.
Alternative Names
Pik3ap1; BcapPhosphoinositide 3-kinase adapter protein 1; B-cell adapter for phosphoinositide 3-kinase; B-cell phosphoinositide 3-kinase adapter protein 1
Species
Mus musculus (Mouse)
Expression Region
1-632
Target Protein Sequence
MVVQPDRIRCGAETTVYIIVRCKLDEKVSTEAEFSPEDSPSIRVEGTLENEYTVSVKAPDLSSGNVSLKVYSGDLVVCETTVSYYTDMEEIGNLLSSAANPVEFMCQAFKIVPYNTETLDKLLTESLKNNIPASGLHLFGINQLEEDDMMTNQRDEELPTLLHFAAKYGLKNLTALLLTCPGALQAYSVANKHGHYPNTIAEKHGFRDLRQFIDEYVETVDMLKTHIKEELMQGEEADDVYESMAHLSTDLLMKCSLNPGCDDELYESMAAFAPAATEDLYVEMLQASAGNPVSGESFSRPTKDSMIRKFLEGNSVKPASWEREQHHPYGEELYHIVDEDETFSVDLANRPPVPVPRPEASAPGPPPPPDNEPYISKVFAEKSQERLGNFYVSSESIRKEPLVRPWRDRPPSSIYDPFAGMKTPGQRQLITLQEQVKLGIVNVDEAVLHFKEWQLNQKKRSESFRFQQENLKRLRESITRRRKEKPKSGKHTDLEITVPIRHSQHLPEKVEFGVYESGPRKSVLPARTELRRGDWKTDSMSSTASSTSNRSSTRSLLSVSSGMEGDNEDNEIPEITRSRGPGPTQVDGAPVVTGTPVGTLERPPRVPPRAASQRPLTRESFHPPPPVPPRGR
Protein Length
Full Length of Isoform 2
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Signaling adapter that contributes to B-cell development by linking B-cell receptor (BCR) signaling to the phosphoinositide 3-kinase (PI3K)-Akt signaling pathway. Has a complementary role to the BCR coreceptor CD19, coupling BCR and PI3K activation by providing a docking site for the PI3K subunit PIK3R1. Alternatively, links Toll-like receptor (TLR) signaling to PI3K activation, a process preventing excessive inflammatory cytokine production. Also involved in the activation of PI3K in natural killer cells. May be involved in the survival of mature B-cells via activation of REL.
Gene References into Functions
  1. identify BCAP as a novel inhibitor of myelopoiesis in the steady state and of emergency myelopoiesis during demand conditions PMID: 28087538
  2. Nck-mediated recruitment of BCAP to the BCR regulates the PI(3)K-Akt pathway in B cells. PMID: 23913047
  3. BCAP is an essential activator of the PI3K pathway downstream of TLR signaling. PMID: 22187458
  4. BCAP is a unique TIR domain-containing toll like receptor (TLR) signaling adapter crucial for linking TLRs to PI3K activation and regulating the inflammatory response. PMID: 22187460
  5. As macrophages engage their pathogenic targets, innate immune receptors trigger increased expression of BCAP, which endows them with the capacity to withstand further challenges from ongoing cellular insults, such as endoplasmic reticulum stress. PMID: 21685326
  6. BCAP-(L) (but not BCAP-(S)) is a negative regulator of the TLR-mediated host defense system in macrophages. PMID: 20728433
  7. plays an immunoregulatory role in B cell development and function--review PMID: 12518443
  8. BCAP and CD19 have complementary roles in BCR-mediated phosphoinositide 3-kinase activation PMID: 18025150
  9. model in which blunting of BCAP-mediated activation signaling in developing NK cells promotes functionality, terminal maturation, and long-term survival PMID: 18337558

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein.
Tissue Specificity
Predominantly expressed in spleen (at protein level). Expressed at lower levels in thymus, liver and lung. Expressed in B-cells, macrophages and natural killer (NK) cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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