Product Details

Purity

>85% (SDS-PAGE)

Target Names

Pitx1

Uniprot No.

P70314

Alternative Names

Pitx1; Bft; Potx; Ptx1; Pituitary homeobox 1; Hindlimb-expressed homeobox protein backfoot; Homeobox protein P-OTX; Homeobox protein PITX1; Paired-like homeodomain transcription factor 1; Pituitary OTX-related factor

Species

Mus musculus (Mouse)

Expression Region

1-315

Target Protein Sequence

MDAFKGGMSL ERLPEGLRPP PPPPHDMGPS FHLARAADPR EPLENSASES SDADLPDKER GGEAKGPEDG GAGSAGCGGG AEDPAKKKKQ RRQRTHFTSQ QLQELEATFQ RNRYPDMSMR EEIAVWTNLT EPRVRVWFKN RRAKWRKRER NQQLDLCKGG YVPQFSGLVQ PYEDVYAAGY SYNNWAAKSL APAPLSTKSF TFFNSMSPLS SQSMFSAPSS ISSMTMPSSM GPGAVPGMPN SGLNNINNLT GSSLNSAMSP GACPYGTPAS PYSVYRDTCN SSLASLRLKS KQHSSFGYGG LQGPASGLNA CQYNS

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Sequence-specific transcription factor that binds gene promoters and activates their transcription. May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb. Can independently activate and synergize with PIT-1 on pituitary-specific target gene promoters, thus may subserve functions in generating both precursor and specific cell phenotypes in the anterior pituitary gland and in several other organs. Can activate pituitary transcription of the proopiomelanocortin gene.

Gene References into Functions

Pitx1 distal enhancer loss have only mild effects on Pitx1 expression in the hindlimb and no overt effects on hindlimb or mandibular morphology. PMID: 29549111
data suggest that PITX1 promotes chondrogenesis and myogenesis in the hindlimb by direct regulation of several key members of the cartilage and muscle transcriptional networks. PMID: 29273440
Results indicate autonomously programmed differences in forelimb and hindlimb cartilage precursors of the limb skeleton are controlled, at least in part, by Pitx1 and suggest this has an important role in generating distinct limb-type morphologies. PMID: 28746404
Pitx1 targets sites that are in a similar chromatin state in Forelimbs (FLs) and hindlimbs and controls expression of patterning genes as well as the chondrogenic program. PMID: 28807899
Pitx1 influences hindlimb morphology both through the activation of hindlimb-specific enhancers as well as through the hindlimb-specific modulation of enhancers that are active in both sets of limbs PMID: 23201014
The results suggest that PITX2 and PITX1 have overlapping roles in thyrotroph function and response to hypothyroidism. PMID: 21964592
PITX1 haploinsufficiency may cause a developmental field defect preferentially affecting the lateral lower leg. PMID: 21775501
It was shown that, firstly, Pitx1 influences hindlimb outgrowth by regulating Tbx4 expression levels and that, subsequently,Pitx1 shapes hindlimb bone and soft tissue morphology independently of Tbx4. PMID: 22071103
These findings provide evidence that c-Abl participates in modulating Pitx1 expression in the apoptotic response to DNA damage. PMID: 20563669
Thus, Pitx1 and Pitx2 genes are required for sustained hindlimb bud growth and formation of hindlimbs PMID: 12441290
expression during cardiovascular development PMID: 12858527
PITX1 autoregulation depends on DNA-binding and trans-activation domains of Pitx1 and it may be responsible for establishment and/or maintenance of the Pitx1 expression domain. PMID: 14512705
Results suggest that pituitary homeobox 1 (Pitx-1) can direct transactivation of the mouse gonadotropin-releasing hormone receptor gene, in part by DNA binding and in part by an action of Pitx-1 as a cofactor for AP-1 PMID: 15226417
Although PITX1 protein is present in expression pattern similar to PITX2 throughout pituitary development, it has less prominent role than PITX2. PMID: 15761027
These results demonstrate that Pitx1, but not Tbx4, determines the morphological identity of hindlimb tissues. PMID: 16989801
Deletion of the Pitx1 genomic locus affects mandibular morphogenesis and expression of the Barx1 and Tbx1 genes. PMID: 18082678

Hide All

Subcellular Location

Nucleus.

Protein Families

Paired homeobox family, Bicoid subfamily

Tissue Specificity

Expressed in primordial Rathke pouch, oral epithelium, first branchial arch, duodenum, and hindlimb.

Database Links

KEGG: mmu:18740

STRING: 10090.ENSMUSP00000021968

UniGene: Mm.135195

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-YP018042MO
MSDS	MSDS
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP018042MO
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP018042MO-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP018042MO
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP018042MO
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Mouse Pituitary homeobox 1 (Pitx1)

Product Details

Customer Reviews and Q&A

Target Background

×CloseMessage