Recombinant Mouse Progressive ankylosis protein (Ankh), partial

Code CSB-YP862360MO
MSDS
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Source Yeast
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Code CSB-EP862360MO
MSDS
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Source E.coli
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Code CSB-EP862360MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862360MO
MSDS
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Source Baculovirus
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Code CSB-MP862360MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ankh
Uniprot No.
Alternative Names
Ankh; AnkProgressive ankylosis protein; Fn54 protein
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulates intra- and extracellular levels of inorganic pyrophosphate (PPi), probably functioning as PPi transporter.
Gene References into Functions
  1. our study provides evidence that ANK plays a critical role in the adipogenic/osteogenic fate decision of adult mesenchymal precursor cells PMID: 28286238
  2. regulates the transport of inorganic pyrophosphate, and Ank knock-out mice feature a rapidly forming and thick cementum and increased cementum regeneration PMID: 21488756
  3. Loss of Ank alters cementoblast gene expression and cervical cementum extracellular matrix composition. PMID: 21389671
  4. the relationship between the type of temporomandibular disorders (TMD) and ANKH polymorphisms PMID: 22003394
  5. Phe377del mutation in ANK causes impaired osteoblastogenesis and osteoclastogenesis resulting in hypomineralization and a high bone mass phenotype. PMID: 21149338
  6. ANK is a positive regulator of osteoblastic and osteoclastic differentiation events toward a mature osteoblastic and osteoclastic phenotype. PMID: 20200976
  7. Analyses on mineralized tissues showed no decrease in mineralization between ank/ank cementum vs. WT controls. Nanoindentation on enamel, dentin or cementum of ank/ank vs. WT molars revealed no significant difference in hardness and elastic modulus. PMID: 19338977
  8. endogenous expression of ANKH mRNA and protein in whole mouse kidney as well as mouse renal epithelial cell lines and a osteoblast cell line. PMID: 19910700
  9. Linked regulatory effects on extracellular PP(i) and OPN expression mediate the ability of PC-1 and ANK to regulate calcification. PMID: 12817751
  10. intra-renal and subcellular localisation of ANK is consistent with pyrophosphate export from collecting duct cells and supports a role for ANK in limiting intra-renal calcium-crystal formation PMID: 17762177
  11. These results revealed a novel function of ANKH in the promotion of early erythroid differentiation and that ank/ank mice have lower serum levels of Epo than the normal littermates, and this is the likely cause of microcytosis in these mutant mice. PMID: 17950726
  12. ANK is a positive regulator of differentiation events towards a mature osteoblastic phenotype. PMID: 18728347
  13. Introduction of a Phe377del mutation in ANK creates a mouse model for craniometaphyseal dysplasia. PMID: 19257826
  14. ANK expression and function in vitro and in vivo are repressed in hypoxic environments and that the effect is regulated by HIF-1. PMID: 19419319

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Involvement in disease
Defects in Ankh are the cause of a generalized, progressive form of arthritis. In ank mice hydroxyapatite crystals develop in articular surfaces and synovial fluid leading to joint space narrowing, cartilage erosion, and formation of bony outgrowths or osteophytes that cause fusion and joint immobility and destruction.
Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
ANKH family
Tissue Specificity
Expressed in heart, brain, liver, spleen, lung, muscle, and kidney of adult animals. Strongly expressed in the developing articular cartilage of joints in the shoulder, elbow, wrist, and digits of the embryo.
Database Links
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