Recombinant Mouse Protein MTO1 homolog, mitochondrial (Mto1)

Code CSB-YP846064MO
MSDS
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Source Yeast
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Code CSB-EP846064MO
MSDS
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Source E.coli
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Code CSB-EP846064MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP846064MO
MSDS
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Source Baculovirus
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Code CSB-MP846064MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mto1
Uniprot No.
Alternative Names
Mto1; Protein MTO1 homolog; mitochondrial
Species
Mus musculus (Mouse)
Expression Region
26-669
Target Protein Sequence
SDSAA PCTPHFDVVV IGGGHAGTEA AAAAARCGSR TLLLTHRVDT IGQMSCNPSF GGIGKGHLMR EVDALDGLCS RICDQSGIHY KVLNRRKGPA VWGLRAQIDR KLYKQNMQKE ILSTPLLTVQ KGAVEDLVLA EPEPGYPGKS RVRGVVLADG STIYAESVIL TTGTFLRGMI IIGLEMHPAG RLGDQPSIGL AQTLEKLGFM VGRLKTGTPP RLGKESINFS ILNKHTPDDP SIPFSFLSDS VWIKPEDQLP CYLTHTNPRV DAIVLENLHL NSHVQETTKG PRYCPSIESK VLRFPNRLHQ VWLEPEGMDS DLIYPQGLSV TLPAELQEKM ITCIRGLEKA KMVHPGYGVQ YDYLDPRQIS PSLETHLVQR LFFAGQINGT TGYEEAAAQG VIAGINASLR VSRKPPFVVS RTEGYIGVLI DDLTTLGTSE PYRMFTSRVE FRLSLRPDNA DTRLTFRAHK EAGCVSSQRF ERALWMKSSL EEGISVLKSI KFSSSKWKKL IPQIPISINR SLPVSALDVL KYEEVDMESL VGVLPEPLEK YTACRELARR LKIEASYESV LSYQLQEIKE VQQDEALQLP HELDYLTIRD VSLSQEVREK LHLSRPQTIG AASRIPGVTP AAIINLLRFV RSTRQSRQQ
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the 5-carboxymethylaminomethyl modification (mnm(5)s(2)U34) of the wobble uridine base in mitochondrial tRNAs.
Gene References into Functions
  1. MTO1 mediates tRNA modification and controls mitochondrial translation rate in a highly tissue-specific manner associated with tissue-specific oxidative phosphorylation defects. PMID: 25552653
  2. here described is an MTO1-deficient mouse model generated by gene trap mutagenesis that mirrors the human phenotype showing complex I defect and cardiomyopathy. PMID: 25506927
  3. These observations suggest that the mouse MTO1 is a structural and functional homolog of yeast MTO1, thereby playing a role in the mitochondrial tRNA modification and protein synthesis. PMID: 14522080
Subcellular Location
Mitochondrion.
Protein Families
MnmG family
Tissue Specificity
Ubiquitously expressed in various tissues, but with markedly elevated expression in tissues of high metabolic rates.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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