Recombinant Mouse Protein lin-28 homolog B (Lin28b)

Code CSB-YP673823MO
MSDS
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Source Yeast
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Code CSB-EP673823MO
MSDS
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Source E.coli
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Code CSB-EP673823MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP673823MO
MSDS
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Source Baculovirus
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Code CSB-MP673823MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lin28b
Uniprot No.
Alternative Names
Lin28b; Protein lin-28 homolog B; Lin-28B
Species
Mus musculus (Mouse)
Expression Region
1-247
Target Protein Sequence
MAEGGASKGE EPEKLPGLAE DEPQVLHGTG HCKWFNVRMG FGFISMISRE GNPLDIPVDV FVHQSKLFME GFRSLKEGEP VEFTFKKSPK GLESIRVTGP GGSPCLGSER RPKGKTLQKR KPKGDRCYNC GGLDHHAKEC SLPPQPKKCH YCQSIMHMVA NCPHKLAAQL PASSQGRQEA ESQPCSSAAP REVGGGHGCT VLFPQEVKSE MAEHSDRSPQ EVSSTKAFAA IGEQNKKGPL IQKRKKT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression. When overexpressed, may stimulate growth of carcinoma cell lines.
Gene References into Functions
  1. Regulating the IL-1beta/miR-i101/Lin28B signaling pathway. PMID: 27562754
  2. The study deciphered the essential role of Lin28B in the pathogenesis of epithelial-mesenchymal transition in idiopathic pulmonary fibrosis, and unraveled a novel mechanism that miR-26a is a modulator of let-7d. PMID: 26787543
  3. Study generated transgenic mice that either overexpressed Lin28b in Math1-positive cerebellar granule neuron precursors or in a broad range of Nestin-positive neural precursors; while overexpression of LIN28B in Nestin-positive cells does not lead to tumor formation, it results in a protracted development of granule cells and inhibitory interneurons and leads to a hypersublobulation of the cerebellar vermis. PMID: 27039094
  4. this study shows that ectopic Lin28b expression reinitiates fetal regenerative potential in a subset of adult hematopoietic stem cells PMID: 27533015
  5. expression of the heterochronic factor Lin28b decreases in common myeloid progenitors during hematopoietic maturation to adulthood in mice. PMID: 27401346
  6. loss of Lin28b expression in fetal T cells leads to increased mature let-7, which causes decreased expression of TGF-betaRI, TGF-betaRIII, and SMAD2 proteins. A reduction in TGF-beta signaling leads to reduced Treg numbers. PMID: 27793996
  7. Lin28b positively regulates the expression of Lin28b itself and cell cycle-related proteins in hepatoblasts by suppressing the maturation of target microRNAs, lethal-7b and miR-125a/b, enabling maintenance of the stem cell properties of hepatoblasts. PMID: 26990797
  8. The identification of the let-7/Lin28 axis as an important regulator of HSC activation as well as its upstream modulators and down-stream targets will provide insights into the involvement of altered microRNA expression in contributing to the pathogenesis of alcoholic liver fibrosis PMID: 28536261
  9. Lin28B/let-7 axis acts as a critical driver of peripheral nervous system myelination PMID: 26466203
  10. These results implicate a key role for the LIN28B/let-7 axis in regulating postnatal supporting cell plasticity. PMID: 26139524
  11. Upregulation of LIN28B is associated with systemic mastocytosis. PMID: 25655194
  12. VH repertoire of Lin28b-induced BM B1a B cells differs from that of normal B1a, suggesting persisting differences from fetal progenitors. PMID: 25753579
  13. MYCN/LIN28B/Let-7/HMGA2 pathway implicated by meta-analysis of GWAS in suppression of post-natal proliferation thereby potentially contributing to aging PMID: 23639551
  14. intestine targeted expression of LIN28B causes intestinal hypertrophy, crypt expansion, and Paneth cell loss PMID: 24142874
  15. Rpl22 inactivation promotes neoplastic transformation by inducing expression of Lin28B PMID: 22976955
  16. Enforced expression of Lin28b leads to impaired T-cell development, release of inflammatory cytokines, and peripheral T-cell lymphoma. PMID: 22723554
  17. study reports Lin28b is expressed in fetal liver and thymus, but not adult bone marrow or thymus; ectopic expression of Lin28 reprograms hematopoietic progenitor cells from adult bone marrow, endowing them with ability to mediate fetal-like lymphopoiesis PMID: 22345399

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Subcellular Location
Nucleus, nucleolus.
Protein Families
Lin-28 family
Database Links

KEGG: mmu:380669

UniGene: Mm.440328

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