Recombinant Mouse Ras-related protein Rab-10(Rab10)

Code CSB-YP019152MO
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Source Yeast
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Code CSB-EP019152MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019152MO
Size Pls inquire other sizes
Source Baculovirus
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Code CSB-MP019152MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Rab10
Uniprot No. P61027
Alternative Names Rab10; Ras-related protein Rab-10
Species Mus musculus (Mouse)
Expression Region 1-200
Target Protein Sequence MAKKTYDLLF KLLLIGDSGV GKTCVLFRFS DDAFNTTFIS TIGIDFKIKT VELQGKKIKL QIWDTAGQER FHTITTSYYR GAMGIMLVYD ITNGKSFENI SKWLRNIDEH ANEDVERMLL GNKCDMDDKR VVPKGKGEQI AREHGIRFFE TSAKANINIE KAFLTLAEDI LRKTPVKEPN SENVDISSGG GVTGWKSKCC
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different set of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion (By similarity). That Rab is mainly involved in the biosynthetic transport of proteins from the Golgi to the plasma membrane. Regulates, for instance, SLC2A4/GLUT4 glucose transporter-enriched vesicles delivery to the plasma membrane. In parallel, it regulates the transport of TLR4, a toll-like receptor to the plasma membrane and therefore may be important for innate immune response. Plays also a specific role in asymmetric protein transport to the plasma membrane within the polarized neuron and epithelial cells. In neurons, it is involved in axonogenesis through regulation of vesicular membrane trafficking toward the axonal plasma membrane while in epithelial cells, it regulates transport from the Golgi to the basolateral membrane. Moreover, may play a role in the basolateral recycling pathway and in phagosome maturation. Finally, may play a role in endoplasmic reticulum dynamics and morphology controlling tubulation along microtubules and tubules fusion.
Gene References into Functions
  1. These results implicate the stress-responsive machinery composed of Rab7L1, LRRK2, phosphorylated Rab8/10, and their downstream effectors in the maintenance of lysosomal homeostasis. PMID: 30209220
  2. Rab10 plays a role in oligodendrocyte precursor cell maturation. PMID: 28132130
  3. SEC16A and RAB10 promote insulin-stimulated mobilization of GLUT4 from a perinuclear recycling endosome/trans Golgi network compartment. PMID: 27354378
  4. Glut4 traffics predominantly through the specialized Rab10-dependent pathway both before and after insulin stimulation. PMID: 26527681
  5. role of Rab10 in mouse embryo PMID: 25860786
  6. These results suggest that the association of Rab10-GTP with Exoc6/6b is a molecular link between insulin signaling and the exocytic machinery in GLUT4 translocation. PMID: 26299925
  7. RAB10 and its GTPase-activating protein AS160 comprise the principal signaling module downstream of insulin receptor activation that regulates the accumulation of GLUT4 transport vesicles at the plasma membrane. PMID: 23804653
  8. Rab10 coordinates with myosin-Va to mediate the final steps of insulin-stimulated vesicle translocation to the plasma membrane. PMID: 22908308
  9. show that continuous replenishment of TLR4 from Golgi to plasma membrane is regulated by the small GTPase Rab10, which is essential for optimal macrophage activation following ipopolysaccharide stimulation. PMID: 20643919
  10. Rab10 is a target of AS160 and a positive regulator of GLUT4 trafficking to the cell surface upon insulin stimulation. PMID: 17403373
  11. the subcellular distribution of Rab10, the fraction of Rab10 in the active GTP form in vivo; Rab10 participates in GLUT4 translocation in 3T3-L1 adipocytes PMID: 18076383

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Subcellular Location Cytoplasmic vesicle membrane, Lipid-anchor, Cytoplasmic side, Golgi apparatus, trans-Golgi network membrane, Endosome membrane, Recycling endosome membrane, Cytoplasmic vesicle, phagosome membrane, Cell projection, cilium, Endoplasmic reticulum membrane
Protein Families Small GTPase superfamily, Rab family
Database Links

KEGG: mmu:19325

STRING: 10090.ENSMUSP00000021001

UniGene: Mm.378993

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