Recombinant Mouse Rho guanine nucleotide exchange factor 25 (Arhgef25)

Code CSB-YP883928MO
MSDS
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Source Yeast
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Code CSB-EP883928MO
MSDS
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Source E.coli
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Code CSB-EP883928MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP883928MO
MSDS
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Source Baculovirus
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Code CSB-MP883928MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arhgef25
Uniprot No.
Alternative Names
Arhgef25; D10Ertd610e; Geft; Rho guanine nucleotide exchange factor 25; Guanine nucleotide exchange factor GEFT; Rac/Cdc42/Rho exchange factor GEFT; RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT; p63RhoGEF
Species
Mus musculus (Mouse)
Expression Region
1-618
Target Protein Sequence
MKPPDRPTPG RTDRILGVMG GMLRACAVPG QEGPPERDPL GPGSTKTESD CIEEDQTGQR EPEVLAWAPQ PESYSIAVSE GSMSASAVSG LAALSGPSSG LSSDPCSPIP PGPVTGLRRW LDHSKHCLSV ETEAESGQTG QCENWTLGPT LTTGQELPEL TLLTTLLEGP GDKAQPAEEE TLSQAPKNEE EQKKMALERS MFVLGELVET ERTYEDDLGQ IVEGYMATMA TQGVPESLRG RDRIVFGNIQ QIYEWHRDYF LQELQQCLKD PDWLAQLFIK HERRLHMYVV YCQNKPKSEH VLSEFGDSYF EELRQQLGHR LQLNDLLIKP VQRIMKYQLL LKDFLKYYRR AGKDTEELEQ AVEVMCFVPK RCNDMMSLGR LRGFEGKLTA QGKLLGQDTF LVTEPEAGGL LSSRGRERRV FLFEQIIIFS EALGGGGRGG AQPGYVYKNS IKVSCLGLEG NLQGNPCRFA LTSRGPEGGI QRYVLQASDP AVSQAWIKQV AQILESQRDF LNALQSPIEY QRRESQTNSL GRPGGPWVGS PGRMRPGDLA QASMHTPING SLPSLLLLPR GEVSRVLLPL DTQALSDTPQ TPHDSPALPT VNTPPCQARL AKLDEDEL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.
Gene References into Functions
  1. In cultured neonatal cardiac fibroblasts, p63RhoGEF regulated the angiotensin II (Ang II)-dependent RhoA activation, the activation of the serum response factor, and the expression and secretion of CTGF. PMID: 26392029
  2. The Geft knockout mice develop normally and exhibit no discernable phenotype, suggesting Geft is dispensable for the development of the second heart field in mouse. PMID: 22449701
  3. Pasteurella multocida toxin prevents osteoblast differentiation by transactivation of the MAP-kinase cascade via the Galpha(q/11)--p63RhoGEF--RhoA axis. PMID: 23696743
  4. GEFT regulation of lens differentiation and eye development occurs through a Rac1-dependent mechanism. PMID: 21663592
  5. Signaling through p63RhoGEF provides a novel mechanism for selective regulation of blood pressure. PMID: 21885830
  6. This protein induces cell proliferation, transformation, and migration. PMID: 12547822

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Subcellular Location
Cytoplasm, myofibril, sarcomere. Cell membrane. Cytoplasm, myofibril.
Tissue Specificity
Highly expressed in excitable tissues, such as brain, heart and muscle. Elevated expression in hippocampus and cerebellum.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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