Recombinant Mouse Serine/threonine-protein kinase OSR1 (Oxsr1)

Code CSB-YP754219MO
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Source Yeast
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Code CSB-EP754219MO
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Source E.coli
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Code CSB-EP754219MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP754219MO
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Source Baculovirus
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Code CSB-MP754219MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Oxsr1
Uniprot No.
Alternative Names
Oxsr1; Osr1; Serine/threonine-protein kinase OSR1; EC 2.7.11.1; Oxidative stress-responsive 1 protein
Species
Mus musculus (Mouse)
Expression Region
2-527
Target Protein Sequence
SEDSSALPW SINRDDYELQ EVIGSGATAV VQAAYCAPKK ERVAIKRINL EKCQTSMDEL LKEIQAMSQC HHPNIVSYYT SFVVKDELWL VMKLLSGGSV LDIIKHIVAK GEHKSGVLDE PTIATILREV LEGLEYLHKN GQIHRDVKAG NILLGEDGSV QIADFGVSAF LATGGDITRN KVRKTFVGTP CWMAPEVMEQ VRGYDFKADI WSFGITAIEL ATGAAPYHKY PPMKVLMLTL QNDPPSLETG VQDKEMLKKY GKSFRKMISL CLQKDPEKRP TAAELLRHKF FQKAKNKEFL QEKILQRAPT ISERSKKVRR VPGSSGRLHK TEDGGWEWSD DEFDEESEEG RAAISQLRSP RVKDSLSSSE LFAAAEPMGT LLQVPEQISA HLPQPAGQMP TQPAQVSLLP PAEPAKPAQA QSSGERSQET KIPISLVLRL RNSKKELNDI RFEFTPGRDT AEGVSQELIS AGLVDGRDLV IVAANLQKIV EEPQSNRSVT FKLASGVEGS DIPDDGKLIG FAQLSIS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Phosphorylates RELL1, RELL2, RELT and PAK1. Phosphorylates PLSCR1 in the presence of RELT.
Gene References into Functions
  1. increased phosphorylation of Na(+)-K(+)-2Cl(-) cotransporter in obesity and identifies a new role for AMP-activated protein kinase in regulating the activity of oxidative stress responsive 1 kinase-related proline-alanine-rich protein kinase PMID: 24808538
  2. OSR1 is expressed in intestine and partial WNK insensitivity of OSR1 increases intestinal Na( )/H( ) exchanger activity and colonic epithelium Na( ) channel activity PMID: 23019198
  3. Data indicate that SPAK and OSR1 function interdependently in the distal convoluted tubule (DCT) to positively regulate thiazide-sensitive sodium-chloride cotransporter, NCC. PMID: 22977235
  4. Partial OSR1 deficiency influences Na(+)/H(+) exchanger activity, ROS formation, and migration of dendritic cells. PMID: 22648948
  5. Two Ste20-like kinases that bind and phosphorylate NKCC1: Ste20-related proline-alanine-rich kinase (SPAK) and oxidative-stress response 1 (OSR1), were identified. PMID: 19307180
  6. In the kidneys, NKCC2 but not NCC is the main target of OSR1 and the reduced p-NKCC2 in KSP-OSR1(-/-) mice may lead to a Bartter-like syndrome. PMID: 21972418
  7. phosphorylation states of NCC and OSR1/SPAK (Gene: Oxsr1, GeneID: 108737) were increased by low-salt diets and decreased by high-salt diets PMID: 18800028
  8. OSR1, a kinase closely related to SPAK, exhibited similar kinase properties and similar functional activation of NKCC1 when coexpressed with WNK4. PMID: 16382158

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Subcellular Location
Cytoplasm.
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, STE20 subfamily
Tissue Specificity
Ubiquitously expressed in all tissues examined, except thymus.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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