Product Details

Purity

>85% (SDS-PAGE)

Target Names

Tbk1

Uniprot No.

Q9WUN2

Alternative Names

Tbk1; Serine/threonine-protein kinase TBK1; EC 2.7.11.1; T2K; TANK-binding kinase 1

Species

Mus musculus (Mouse)

Expression Region

1-729

Target Protein Sequence

MQSTSNHLWL LSDILGQGAT ANVFRGRHKK TGDLYAVKVF NNISFLRPVD VQMREFEVLK KLNHKNIVKL FAIEEETTTR HKVLIMEFCP CGSLYTVLEE PSNAYGLPES EFLIVLRDVV GGMNHLRENG IVHRDIKPGN IMRVIGEDGQ SVYKLTDFGA ARELEDDEQF VSLYGTEEYL HPDMYERAVL RKDHQKKYGA TVDLWSVGVT FYHAATGSLP FRPFEGPRRN KEVMYKIITG KPSGAISGVQ KAENGPIDWS GDMPLSCSLS QGLQALLTPV LANILEADQE KCWGFDQFFA ETSDVLHRMV IHVFSLQHMT AHKIYIHSYN TAAVFHELVY KQTKIVSSNQ ELIYEGRRLV LELGRLAQHF PKTTEENPIF VTSREQLNTV GLRYEKISLP KIHPRYDLDG DASMAKAVTG VVCYACRTAS TLLLYQELMR KGVRWLVELV KDDYNETVHK KTEVVITLDF CIRNIEKTVK VYEKLMKVNL EAAELGEISD IHTKLLRLSS SQGTIESSLQ DISSRLSPGG LLADTWAHQE GTHPRDRNVE KLQVLLNCIT EIYYQFKKDK AERRLAYNEE QIHKFDKQKL YYHATKAMSH FSEECVRKYE AFKDKSEEWM RKMLHLRKQL LSLTNQCFDI EEEVSKYQDY TNELQETLPQ KMLAASGGVK HAMAPIYPSS NTLVEMTLGM KKLKEEMEGV VKELAENNHI LERFGSLTMD GGLRNVDCL

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine/threonine kinase that plays an essential role in regulating inflammatory responses to foreign agents. Following activation of toll-like receptors by viral or bacterial components, associates with TRAF3 and TANK and phosphorylates interferon regulatory factors (IRFs) IRF3 and IRF7 as well as DDX3X. This activity allows subsequent homodimerization and nuclear translocation of the IRFs leading to transcriptional activation of pro-inflammatory and antiviral genes including IFNA and IFNB. In order to establish such an antiviral state, TBK1 form several different complexes whose composition depends on the type of cell and cellular stimuli. Thus, several scaffolding molecules including FADD, TRADD, MAVS, AZI2, TANK or TBKBP1/SINTBAD can be recruited to the TBK1-containing-complexes. Plays a key role in IRF3 activation: acts by first phosphorylating innate adapter proteins MAVS, STING1 and TICAM1 on their pLxIS motif, leading to recruitment of IRF3, thereby licensing IRF3 for phosphorylation by TBK1. Under particular conditions, functions as a NF-kappa-B effector by phosphorylating NF-kappa-B inhibitor alpha/NFKBIA, IKBKB or RELA to translocate NF-Kappa-B to the nucleus. Restricts bacterial proliferation by phosphorylating the autophagy receptor OPTN/Optineurin on 'Ser-177', thus enhancing LC3 binding affinity and antibacterial autophagy. Phosphorylates SMCR8 component of the C9orf72-SMCR8 complex, promoting autophagosome maturation. Phosphorylates and activates AKT1. Seems to play a role in energy balance regulation by sustaining a state of chronic, low-grade inflammation in obesity, wich leads to a negative impact on insulin sensitivity.

Gene References into Functions

In response to the presence of cytosolic DNA, STING translocates from the endoplasmic reticulum (ER) to the Golgi, and activates TANK-binding kinase 1 (TBK1), a cytosolic kinase that is essential for the activation of STING-dependent downstream signaling. TBK1 binds to STING at the Golgi, not at the ER. PMID: 29870684
Chronic innate immune activation of TBK1 suppresses mTORC1 activity, leading to dysregulated cellular metabolism. PMID: 28069950
IL-17 inhibits adipogenesis where a lack of IL-17 ameliorates glucose metabolism. As well, the inhibition of TBK1 reduces inflammation induced by IL-17. Therefore, IL-17 may be involved in the development of obesity and metabolic dysfunction in a TBK1-dependent manner. PMID: 28237848
The TBK1 Y179A mutant failed to rescue type I IFN production by virally infected RAW264.7 macrophages deficient in TBK1 PMID: 28049762
HERP Binds TBK1 To Activate Innate Immunity and Repress Virus Replication in Response to Endoplasmic Reticulum Stress PMID: 28954889
these studies reveal an additional regulatory function of TRIM8 in innate immune responses: TRIM8 catalyzes polyubiquitination of TRIF, resulting in disruption of TRIF-TBK1 interaction PMID: 28747347
TBK1 regulates p16 expression and retinal ganglion cell senescence. PMID: 28425986
functions in dendritic cells to regulate T cell homeostasis, autoimmunity, and antitumor immunity PMID: 28356390
USP38 inhibits type I interferon signaling by editing TBK1 ubiquitination through NLRP4 signalosome. PMID: 27692986
Innate immune responses to adenovirus vectors are largely TBK1-dependent in the spleen but TBK1-independent in the liver PMID: 26489883
This study suggest that TBK1 exerts pronociceptive effects in inflammatory nociception which are due to both modulation of NF-kappaB dependent genes and regulation of MAPKs and c-fos. PMID: 25997745
TRIM27 inhibits VSV infection-induced type I IFN production by promoting TBK1 degradation. PMID: 26358190
Suggest that by reducing production of cAMP in adipocytes, PDE3B, IKKepsilon and TBK1 may contribute to the repression of energy expenditure during obesity. PMID: 24368730
TBK1 has a role in regulating T-cell activation and migration PMID: 25606824
findings demonstrate a novel regulatory circuit in which STING and TBK1 reciprocally regulate each other to enable efficient antiviral signaling activation, and PPM1A dephosphorylates STING and TBK1 PMID: 25815785
The results highlight the importance of the C-terminal region in the functional activity of IKKepsilon in innate immune response.Interestingly, corresponding region and residues are not required for activation of downstream signaling by TBK1. PMID: 24722368
data elucidated the cell type-specific subcellular localization of p-TBK1 and a cell type-specific role of IPS-1 in TBK1 activation in response to cytoplasmic viral DNA. PMID: 24349538
TBK1 plays a critical role in mediating the effects of measles virus nucleocapsid protein on osteoclast differentiation and on the expression of IL-6, a key contributor to the pagetic osteoclast phenotype. PMID: 23794264
TANK-binding kinase 1 associated with STING was found to facilitate dsDNA-mediated canonical activation of NF-kappaB as well as IRF3 to promote proinflammatory gene transcription. PMID: 24600004
essential for signal transduction in Toll-like receptor-mediated glycolytic flux and dendritic cell activation PMID: 24562310
Knockdown of TBK1, STING, and cGAS results in a dramatic reduction in the activation of phosphorylated IRF3 following exposure to adenovirus. PMID: 24198409
The overall structures of the TBK1 monomer and its dimer are different from IKKbeta in the arrangements of the three domains and in dimer formation. PMID: 23746807
NEMO binds ubiquitinated TANK-binding kinase 1 (TBK1) to regulate innate immune responses to RNA viruses. PMID: 23028469
Activation of IRF3 by thapsigargin requires TANK-binding kinase (TBK)1 and interferon stimulator STING. PMID: 23028052
The kinase TBK1 controls IgA class switching by negatively regulating noncanonical NF-kB signaling. PMID: 23023393
TBK-1 was necessary for autophagic maturation, it coordinated assembly and function of the autophagic machinery and phosphorylated the autophagic adaptor p62 (sequestosome 1) on Ser-403, a residue essential for its role in autophagic clearance. PMID: 22921120
Herpes simplex virus gamma(1)34.5 negatively regulates TBK1, which contributes viral replication. PMID: 22171259
TBK1 activation was strictly dependent on the integrity of the TBK1/TANK interaction PMID: 21931631
OPTN binds to polyubiquitylated species formed in response to LPS and poly(I:C), enhancing the activation of TBK1 that is required for optimal phosphorylation of IRF3 and production of IFNbeta. PMID: 21862579
IkappaB kinase epsilon and TANK-binding kinase 1 activate AKT by direct phosphorylation. PMID: 21464307
IKKepsilon/TBK1 mediate the activation of Pellino 1's E3 ligase activity, as well as inducing the transcription of its gene and protein expression in response to TLR3 and TLR4 agonists. PMID: 21204785
TBK1 regulates phosphorylation of protein-serine-threonine kinase Akt, a downstream molecule of TRIF/TANK-binding kinase 1; TBK1 has an important novel role in the activation of IFN regulatory factor 3 (IRF3) by Toll-like receptor (TLR)3 and -4 agonists. PMID: 21106850
Mice lacking Tbk1 activity exhibit immune cell infiltrates in multiple tissues and increased susceptibility to LPS-induced lethality. PMID: 20651301
Results point to SHIP-1 as a critical negative regulator of IFN-beta production downstream of TLR3 through the regulation of TBK1 localization and activity. PMID: 20100929
TBK1 plays an important role in the Toll-like receptor-mediated IFN response. PMID: 15210743
This study describes a novel signal transduction system involving TBK1 that is used by Listeria monocytogenes to activate type I interferon expression. PMID: 15661922
interferon-A genes differentially affects virus-induced expression and responsiveness to TBK1 and IKKepsilon PMID: 16380379
These observations define the mechanistic contribution of RalGTPases to cancer cell survival and reveal the RalB/Sec5 effector complex as a component of TBK1-dependent innate immune signaling. PMID: 17018283
TANK-binding kinase 1 is essential and for the regulation of IFN responses in innate immune cells except plasmacytoid DCs. PMID: 17056502
data demonstrate a requirement for TBK1 in control of bacterial infection distinct from its established role in antiviral immunity. PMID: 17335348
Outlines a novel pathway for IRF-3 activation by Tbk1 providing new insights into the mechanism of a new class of chemotherapeutic drugs such as DMXAA. PMID: 17562815
The ubiquitin-like domain is a regulatory component of the TBK1/IKK-i kinases involved in the control of the kinase activation, substrate presentation and downstream signaling pathways. PMID: 17599067
TBK1 is a key signalling molecule for DNA-vaccine-induced immunogenicity, by differentially controlling DNA-activated innate immune signalling through haematopoietic and non-haematopoietic cells PMID: 18256672
CYLD as a key negative regulator of IKKepsilon/TBK1 and suggest a role for CYLD in the control of RIG-I ubiquitination. PMID: 18467330
These results highlight the contribution of water channels to promoting PCV membrane integrity, and reveal an unexpected role for TBK1 and AQP1 in restricting bacterial pathogens to the vacuolar compartment. PMID: 18665841
Upon Sendai virus (SeV) infection, TBK1s is induced in both human and mouse cells and binds to RIG-1, disrupting the interaction between RIG-I and VISA PMID: 18977754
control of TBK1-mediated cell signaling by the gamma(1)34.5 protein contributes to herpes simplex virus infection PMID: 19010780
TBK1 rescued IFN responses to transfected B-DNA PMID: 19201879
TBK1 could be involved in the insulin resistance mechanism related with IR Ser994 phosphorylation in a genetic model of diabetes. PMID: 19251743

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Subcellular Location

Cytoplasm.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily

Database Links

KEGG: mmu:56480

STRING: 10090.ENSMUSP00000020316

UniGene: Mm.34580

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Code	CSB-YP894827MO
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Code	CSB-EP894827MO
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Recombinant Mouse Serine/threonine-protein kinase TBK1 (Tbk1)

Product Details

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Target Background

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