Product Details

Purity

>85% (SDS-PAGE)

Target Names

Slamf1

Uniprot No.

Q9QUM4

Alternative Names

Slamf1; Slam; Signaling lymphocytic activation molecule; SLAM family member 1; CD antigen CD150

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Self-ligand receptor of the signaling lymphocytic activation molecule (SLAM) family. SLAM receptors triggered by homo- or heterotypic cell-cell interactions are modulating the activation and differentiation of a wide variety of immune cells and thus are involved in the regulation and interconnection of both innate and adaptive immune response. Activities are controlled by presence or absence of small cytoplasmic adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2. SLAMF1-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAMF1 signaling seem to exist: one depending on SH2D1A (and perhaps SH2D1B) and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. Initially it has been proposed that association with SH2D1A prevents binding to inhibitory effectors including INPP5D/SHIP1 and PTPN11/SHP-2. However, signaling is also regulated by SH2D1A which can simultaneously interact with and recruit FYN which subsequently phosphorylates and activates SLAMF1. Mediates IL-2-independent proliferation of activated T-cells during immune responses and induces IFN-gamma production. Downstreaming signaling involves INPP5D, DOK1 and DOK2 leading to inhibited IFN-gamma production in T-cells, and PRKCQ, BCL10 and NFKB1 leading to increased T-cell activation and Th2 cytokine production. Promotes T-cell receptor-induced IL-4 secretion by CD4(+) cells. Inhibits antigen receptor-mediated production of IFN-gamma, but not IL-2, in CD4(-)/CD8(-) T-cells. Required for IL-4 production by germinal centers T follicular helper (T(Fh))cells. May inhibit CD40-induced signal transduction in monocyte-derived dendritic cells. May play a role in allergic responses and may regulate allergen-induced Th2 cytokine and Th1 cytokine secretion. In conjunction with SLAMF6 controls the transition between positive selection and the subsequent expansion and differentiation of the thymocytic natural killer T (NKT) cell lineage. Involved in the peripheral differentiation of indifferent natural killer T (iNKT) cells toward a regulatory NKT2 type. In macrophages involved in down-regulation of IL-12, TNF-alpha and nitric oxide in response to lipopolysaccharide (LPS). In B-cells activates the ERK signaling pathway independently of SH2D1A but implicating both, SYK and INPP5D, and activates Akt signaling dependent on SYK and SH2D1A. In conjunction with CD84/SLAMF5 and SLAMF6 may be a negative regulator of the humoral immune response.; (Microbial infection) Involved in innate immune response against Gram-negative bacteria in macrophages; probably recognizes OmpC and/or OmpF on the bacterial surface, regulates phagosome maturation and recruitment of the PI3K complex II (PI3KC3-C2) leading to accumulated of PdtIns(3)P and NOX2 activity in the phagosomes.

Gene References into Functions

the Slam locus has an overall inhibitory role during NK cell activation that is solely dependent on 2B4. This effect is influenced by cytokines and leads to suppression of LFA-1 activity. PMID: 27573813
CRISPR-Mediated Slamf1Delta/Delta Slamf5Delta/Delta Slamf6Delta/Delta Triple Gene Disruption Reveals NKT Cell Defects but Not T Follicular Helper Cell Defects. PMID: 27223891
We demonstrated that Bacille Calmette-Guerin infection significantly upregulated SLAMF1, which enhanced inflammatory response by activating the NF-kappaB signaling pathway and facilitated bacterial clearance in BCG-infected RAW264.7 cells and mice. PMID: 26253450
Slamf1 and Slamf8 govern ROS-dependent innate immune responses of myeloid cells. PMID: 25799045
The combined effects of RIIB deletion and pathogenic SLAM can lead to severe lupus nephritis in the B6 genetic background. PMID: 25339546
The goal of the current study was to determine whether Slam haplotype affected NKT and Vgamma4+ T-cell responses subsequent to coxsackievirus b3 infection. PMID: 23195432
Signaling lymphocyte activation molecule regulates development of colitis in mice. PMID: 22960654
Slamf1, which controls phagosomal/lysosomal fusion and phagosomal NADPH-oxidase activity, is required for T.cruzi replication in macrophages and dendritic cells, but not in other cells, which do not express the receptor. PMID: 22807679
conclude that Slamf1 recruits a subset of Vps34-associated proteins, which is involved in membrane fusion and NOX2 regulation PMID: 22493499
Slamf1 is a bacterial sensor that regulates intracellular enzyme activities involved in the removal of Gram-negative bacteria. PMID: 20818396
SLAM signaling drives most of (interleukin) IL-4 production by germinal center T follicular helper CD4 cells. PMID: 20525889
Polymorphisms that distinguish two Slam haplotypes significantly modulate the innate immune response in vivo through their effect on natural killer T (NKT) cells. PMID: 20530260
role of SAP in murine antigen-mediated T-cell proliferation and interferon gamma production PMID: 12351401
Coreceptor SLAM plays a central role at the interface of acquired and innate immune responses. PMID: 15123745
Cell surface receptors of the SLAM family, including CD150, CD244, and CD48, were differentially expressed among functionally distinct hematopoietic progenitor cells. PMID: 15989959
control of NKT cell numbers attributed to the Nkt1 gene is mediated by differential expression of Slamf1 and an additional contribution by Slamf6. PMID: 17237411
Both CD150(+) and CD150(-) cells can be found within the stem cell population population and both populations can contribute to long-term multilineage reconstitution. PMID: 18055867
data suggest that the genetic Slamf1 defect in NOD mice also affects SLAM expression on other immune cells such as the mDC, thus critically impairing the peripheral differentiation of iNKT cells toward a regulatory NKT2 type. PMID: 18606638

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Database Links

KEGG: mmu:27218

STRING: 10090.ENSMUSP00000015460

UniGene: Mm.103648

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Recombinant Mouse Signaling lymphocytic activation molecule (Slamf1), partial

Product Details

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Target Background

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