Recombinant Mouse Slit homolog 1 protein (Slit1), partial

Code CSB-YP800323MO
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP800323MO
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP800323MO-B
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP800323MO
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP800323MO
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Slit1
Uniprot No.
Alternative Names
Slit1; Kiaa0813; Slit homolog 1 protein; Slit-1
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
Gene References into Functions
  1. Contralateral migration of oculomotor neurons is regulated by Slit/Robo signaling. Results demonstrate that a migratory subset of motor neurons respond to floor plate-derived Slit repulsion to properly control the timing of contralateral migration. PMID: 27770832
  2. While Slit1 and Robo2 are only expressed in peripheral axons and their cell bodies, Slit2, Slit3 and Robo1 are also expressed in satellite cells of the dorsal root ganglion, Schwann cells and fibroblasts of peripheral nerves. PMID: 28234971
  3. These observations from mutant Slit and Robo mice give great support for Slit/Robo role in neural crest cell migration during Enteric Nervous System development. PMID: 25490618
  4. Data indicate the roles of Slit1/Robo1 and Netrin1/DCC signals in positioning motor neuron cell bodies. PMID: 25530182
  5. a role for Slit1 in corpus callosum development PMID: 22349628
  6. Robo-2-mediated targeting of P2 axons along the dorsoventral axis of the OB is controlled by Slit-1 expression PMID: 22981605
  7. This study demonistrated that production of IPCs is enhanced in Robo1/2 and Slit1/2 mutants, suggesting that Slit/Robo signaling modulates the transition between primary and intermediate progenitors. PMID: 23083737
  8. Disruption of either the Robo1 or Slit1 genes accelerates progression of thalamocortical axons in vivo. PMID: 22772332
  9. Slit1 has a dual context-dependent role in thalamocortical axons formation PMID: 22000108
  10. Robo1 and Robo2 are expressed in the nucleus origin of the tract of the postoptic commissure TPOC (nTPOC), while Slit expression domains flank the TPOC trajectory. PMID: 21688288
  11. Nkx2.9 controls SACMN axon exit from the mammalian spinal cord by regulating Robo-Slit signaling PMID: 22399681
  12. Data show that only Nck2 is required for the Slit1-induced changes in cortical neuron morphology in vitro. PMID: 21600986
  13. These results indicate that Slit1/2 - Robo1/2 signaling is critical during the initial establishment of dopaminergic pathways, with roles in the dorsoventral positioning and precise pathfinding of these ascending longitudinal axons. PMID: 21118670
  14. The results of this study suggested that the Slit1 secreted by new neurons alters the morphology of astrocytes through the astrocytes' Robo receptors, thereby promoting the fast migration of new neurons in contact with the astrocytes. PMID: 20670830
  15. role in antagonizing netrin-1 attractive effects during migration of inferior olivary neurons PMID: 12051827
  16. Slit proteins control the development of the lateral olfactory tract PMID: 12097499
  17. Analysis of alternative splicing and conserved domains in human and mouse slit genes PMID: 12141424
  18. our results suggest that Slit1 signaling may be involved in the regulation of molar tooth shape by regulating epithelial cell proliferation and formation of EFA of the crown. PMID: 12591596
  19. Data suggest that the Slit family of axon guidance molecules (Slit 1-3) and their Robo 1 and 2 receptors contribute to the topographic targeting of basal vomeronasal axons. PMID: 12954717
  20. The Slit receptors Robo2 and Robo3/Rig-1 are expressed in the subventricular zone and the rostral migratory stream and Slit1 and Slit2 are present in the adult septum and are responsible for both the septum and the CP repulsive activity in vitro PMID: 14960623
  21. Experiments with triple mutant mice demonstrate a key role for Slit1 signaling in vertebrate midline commissural axon guidance. PMID: 15091338
  22. In contrast to Sema3F, Slit-1 is dispensable for fasciculation of basal vomeronasal neuron axons but is critical for targeting these axons to the posterior AOB PMID: 15483127
  23. Slit-mediated repolarization consisted of growth of a new process from the previous trailing edge, then reorientation of the neuronal centrosome followed by nuclear translocation in the reverse direction. PMID: 16682216
  24. Slit1 helps prevent retinal ganglion cell axons from crossing the diencephalic/telencephalic boundary in the developing optic tract. PMID: 16828733
  25. We also have found that the lens, which because of its peripheral location within the developing eye is ideally located to influence the initial direction of RGC axon outgrowth, secretes Slit2. PMID: 16885222
  26. Requirement for Slit-1 in zonal segregation of olfactory sensory neuron axons in the main olfactory bulb. PMID: 17715346
  27. The role of Slit1-Robo signaling in the generation, migration and morphological differentiation of cortical interneurons is reported. PMID: 18054781
  28. Slit1 and Slit2 cooperate with Robo1 and Robo2 to control the convergence ofolfactory receptor neuron (ORN) axons to the olfactory bulb and the precise targeting of ORN axons to specific glomeruli. PMID: 18417704
  29. Slit1;Slit2 double mutants had severe longitudinal errors, particularly for ventral axons, including midline crossing and wandering longitudinal trajectories PMID: 18842816
  30. results suggest strongly that Robo2 redundancy with other guidance signals rather than different receptor utilisation is the most likely explanation for the regional specificity of Slit function during intraretinal axon pathfinding PMID: 19782674
  31. expressed in the primary enamel knot of the bud and cap stage tooth germ and later the expression continued in the secondary enamel knots of the late cap and bell stage tooth. PMID: 11520671

Show More

Hide All

Subcellular Location
Secreted.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X