Recombinant Mouse Solute carrier family 12 member 2 (Slc12a2), partial

Code CSB-YP021383MO
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Source Yeast
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Code CSB-EP021383MO
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Source E.coli
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Code CSB-EP021383MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021383MO
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Source Baculovirus
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Code CSB-MP021383MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Slc12a2
Uniprot No.
Alternative Names
Slc12a2; Nkcc1; Solute carrier family 12 member 2; Basolateral Na-K-Cl symporter; Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume.
Gene References into Functions
  1. Data show that the Na(+)/K(+)/2Cl(-) cotransporter (NKCC1) expressed in the luminal membrane of choroid plexus contributes approximately half of the cerebrospinal fluid (CSF) production. PMID: 29867199
  2. Study showed that there is a transient dysregulation in the levels of NKCC1 and KCC2 at disease onset, the time point when heightened nociceptive behaviors are most pronounced in mice with experimental autoimmune encephalomyelitis. PMID: 28057537
  3. This study identify neuronal up-regulation of NKCC1 and its mediation by TGFbeta, as a potential and important mechanism in the early post-traumatic seizures, and demonstrate the therapeutic potential of blocking this pathway. PMID: 27586142
  4. Study suggests that astaxanthin may exert neuroprotection following traumatic brain injury by ameliorating AQP4/NKCC1-mediated cerebral edema, and NKCC1 inactivation inhibitions the upregulation of AQP4 after traumatic brain injury. PMID: 27581370
  5. GABAA receptor (GABAAR) and the Na(+)-K(+)-2Cl(-) cotransporter (NKCC1), but not the K(+)-Cl(-) cotransporter (KCC2), were expressed in the terminals of the CRH neurons at the median eminence (ME). In contrast, CRH neuronal somata were enriched with KCC2 but not with NKCC1. PMID: 27540587
  6. These results identify NKCC1 as a novel target for Nedd4L-mediated down-regulation in vivo, which modulates ion and fluid transport in the distal colon together with epithelial Na(+) channel (ENaC). PMID: 28087701
  7. NKCC1 inhibition protects neurons from traumatic brain injury via regulation of the ERK signal pathway. PMID: 26854573
  8. We conclude that TRPV2 is involved in osmosensation in skeletal muscle fibres, acting in concert with P-SPAK-activated NKCC1 PMID: 26108786
  9. KCC2 expression supersedes NKCC1 in mature fiber cells in mouse and rabbit lenses. PMID: 26539026
  10. GnRH neurons were immunopositive for NKCC1 and AE2. PMID: 26110920
  11. NKCC1 may have a role in periventricular leukomalacia, as shown by bumetanide protection against white matter injury in a rodent model PMID: 25585037
  12. Repeated stress decreased KCC2 expression and increased NKCC1 expression in membranes of granular and pyramidal cells in the hippocampus. PMID: 26239662
  13. Data indicate that ion transporter NKCC1 is involved in chloride accumulation but also reveals an impact in neurogenesis. PMID: 25713142
  14. NKCC1 appeared down regulated in age related hearing loss. PMID: 25220727
  15. The FAK inhibitor PF-573,228 augmented shrinkage-induced Src phosphorylation, and inhibited shrinkage-induced NKCC1 activity. PMID: 25377086
  16. in addition to SPAK and OSR1, WNK4 is able to anchor itself to the N-terminal domain of NKCC1 and to promote cotransporter activation. PMID: 24811174
  17. Fluid secretion is enhanced in a hypotonic challenge through the activation of NKCC1 in the mouse submandibular salivary gland. PMID: 24623142
  18. angiotensin II activates the WNK-SPAK-NKCC1 phosphorylation cascade through the angiotensin II type 1 receptor. PMID: 24019400
  19. Our results characterize an innovative mechanism of cardiogenic edema formation and identify NKCC1 as a unique therapeutic target in cardiogenic lung edema. PMID: 23645634
  20. Capsaicin inhibits chloride secretion in part by causing NKCC1 internalization, but by a mechanism that appears to be independent of TRPV1. PMID: 23139219
  21. This study demonistrated that NKCC1 knockdown decreases neuron production through GABA(A)-regulated neural progenitor proliferation and delays dendrite. PMID: 23015452
  22. Slc12a2 gene is not necessary for insulin secretion and that its absence increases beta-cell secretory capacity. PMID: 22872759
  23. NKCC1 and anion exchangers display functional activity to accumulate chloride ion under near physiological conditions (artificial cerebrospinal fluid). The dual Cl- accumulation mechanism is essential to homeostasis of neurogenesis in postnatal forebrain. PMID: 22390178
  24. globally, OSR1 is involved in the regulation of BP and renal tubular Na(+) reabsorption mainly via the activation of NKCC1 and NKCC2 PMID: 21972418
  25. It was shown that the behavior of the equations in Lineweaver-Burke plots are consistent with a model where Cl- binds first, followed by Na+, a second Cl-, and then K+. PMID: 21775703
  26. Findings uncover a vascular signaling pathway linking alpha(1)-adrenergic receptors and pressure to WNK1, SPAK, and NKCC1 and may, thus, significantly broaden the comprehension of the regulatory mechanisms of vascular tone in arterial hypertension. PMID: 21768522
  27. Expression of KCNQ1 and NKCC1 protein in the stria vascularis of C57BL/6J mice decreases with age. PMID: 21426709
  28. Ion channel NKCC1 and alpha2 Na, K-ATPase played important roles in the inner ear potassium recycling. PMID: 17111813
  29. NKCC1 gene takes part in the pathogenesis of age-related hearing loss. PMID: 17007383
  30. This study demonistrated that NKCC1 controls GABAergic signaling and neuroblast migration in the postnatal forebrain. PMID: 21284844
  31. Both protein and mRNA expression of alpha1 and alpha2 isoforms of Na,K-ATPase and NKCC1 in the lateral wall were dramatically reduced following a long-term deafening PMID: 21094218
  32. Kanamycin could inhibit the expression of NKCC1 in stria vascularis. PMID: 16646244
  33. In order to identify the molecular determinants of NKCC1-specific activation, chimeras of the mouse NKCC1 and the rat NKCC2 were created and expressed in X. laevis oocytes. PMID: 20530115
  34. phosphorylation-induced activation of NKCC1 by osmotic shrinkage does not involve AMP-activated protein kinase and is likely to be due to STE20/SPS1-related proline/alanine-rich kinase activation PMID: 20442269
  35. It is critical for the two main sites of NKCC1 phosphorylation (T206 and T211) to be separated by four amino acid residues for SPAK target recognition. PMID: 20463172
  36. NKCC1 activity strongly contributes to the intracellular chloride accumulation induced by recurrent seizures. PMID: 20810895
  37. data suggest that KCC2 and NKCC1 in Vc may play a critical role in the nociception and transmission of dental pain during pulpal inflammation PMID: 20067823
  38. This study suggests that stimulation of NKCC1 is required for high-[K(+)](o)-induced swelling, which contributes to glutamate release from astrocytes under high [K(+)](o). PMID: 11940530
  39. Mouse mammary epithelial cells express the Na-K-Cl cotransporter, NKCC1: characterization, localization, and involvement in ductal development and morphogenesis. PMID: 12040017
  40. NKCC1 can be important for the maintenance of normal blood pressure and vascular tone. PMID: 12384462
  41. interact with the stress-related kinases Ste20-related proline-alanine-rich kinase (SPAK) and oxidative stress response 1 (OSR1) PMID: 12386165
  42. Normal NKCC1 levels, reduced cotransport, but activation by cAMP in colonic crypts from CFTR (-/-) mice. No dysfunction of Nkcc1 in cystic fibrosis intestine. Direct activation of colonic NKCC1 by increase in intracellular cAMP. PMID: 12692180
  43. NKCC1 KO mice showed a reduction in stroking hyperalgesia (touch-evoked pain) compared to WT and HE mice but no differences were detected between the three groups in the expression of punctate hyperalgesia. PMID: 15135928
  44. Stimulation of NKCC1 activity leads to Na+ accumulation after oxygen and glucose deprivation followed by reoxygenation. PMID: 15509746
  45. These data suggest that NKCC1 plays an important role in fluid secretion by the exorbital gland of mice. PMID: 15917300
  46. WNK4 interacts with SPAK, which in turn phosphorylates and activates NKCC1 PMID: 15930150
  47. NKCC contributes importantly to secretions of Na+, K+, Cl-, fluid, and pepsinogen by the gastric mucosa through a process that is electrogenic in character and independent of acid secretion PMID: 16093421
  48. data suggest that a functional NKCC1 suppresses basal renin release, at least in part, through a direct effect on juxtaglomerular granular cells PMID: 16106034
  49. These data support the hypothesis that NKCC1 expressed in myenteric ICC is involved in the mechanism of slow waves in the murine jejunum. PMID: 16123204
  50. Increased Na+ transporter expression, increased plasma renin, and reduced plasma ANP, as observed in NKCC1 null mice, should increase vascular volume and blood pressure, thus minimizing hypotension. PMID: 16159893

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Subcellular Location
Basolateral cell membrane; Multi-pass membrane protein.
Protein Families
SLC12A transporter family
Tissue Specificity
Widely expressed. High expression found in the cochlea, cochlear lateral wall, and the choroid plexus. Lower expression found in the cerebellum and the cortex.
Database Links
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