Recombinant Mouse Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1 (Sohlh1)

Code CSB-YP743595MO
MSDS
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Source Yeast
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Code CSB-EP743595MO
MSDS
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Source E.coli
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Code CSB-EP743595MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP743595MO
MSDS
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Source Baculovirus
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Code CSB-MP743595MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sohlh1
Uniprot No.
Alternative Names
Sohlh1; Gm110; Tohlh1; Spermatogenesis- and oogenesis-specific basic helix-loop-helix-containing protein 1
Species
Mus musculus (Mouse)
Expression Region
1-357
Target Protein Sequence
MASGGHERAN EDYRVSGITG CSKTPQPETQ DSLQTSSQSS ALCTAPVAAA NLGPSLRRNV VSERERRRRI SLSCEHLRAL LPQFDGRRED MASVLEMSVY FLQLAHSMDP SWEQLSVPQP PQEMWHMWQG DVLQVTLANQ IADSKPDSGI AKPSAVSRVQ DPPCFGMLDT DQSQATERES ELLERPSSCP GHRQSALSFS EPESSSLGPG LPPWIPHSWQ PATPEASDIV PGGSHQVASL AGDPESSGML AEEANLVLAS VPDARYTTGA GSDVVDGAPF LMTTNPDWWL GSVEGRGGPA LARSSPVDGA EPSFIGDPEL CSQELQAGPG ELWGLDFGSP GLALKDEADS IFPDFFP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription regulator of both male and female germline differentiation. Suppresses genes involved in spermatogonial stem cells maintenance, and induces genes important for spermatogonial differentiation. Coordinates oocyte differentiation without affecting meiosis I.
Gene References into Functions
  1. Sohlh1 represents the first testis-specific bHLH transcription factor that is essential for spermatogonial differentiation. PMID: 16564520
  2. Sohlh1 and Lhx8 are two germ cell-specific, critical regulators of oogenesis PMID: 16690745
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
In males, it is mainly expressed in testis, while in females it is mainly expressed in ovary. In testis, it is exclusively expressed in spermatogonia, with a preference for prespermatogonia and type A spermatogonia. In ovary, it is detected in germ cell c
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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