Recombinant Mouse Stimulated by retinoic acid gene 8 protein(Stra8)

Code CSB-YP022873MO
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Source Yeast
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Code CSB-EP022873MO
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Source E.coli
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Code CSB-EP022873MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022873MO
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Source Baculovirus
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Code CSB-MP022873MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Stra8
Uniprot No. P70278
Alternative Names Stra8; Stimulated by retinoic acid gene 8 protein
Species Mus musculus (Mouse)
Expression Region 1-393
Target Protein Sequence MATPGEGNQPSDDGAPQPLAQLQKLEPRVVRRRLSQARHRATLVGLFNNLRKAVYSQSDI TASKWQVLNRTKIHIQEQEESLDKLLKLKASFNLQDGNPNSLEEVKEEYARMYSENDSVF LNSFLQDSPPEWFPSEAVGPDAEEEGEEEGEEEGEEGEEEEEGDEEGEEEEENGEEREVE EYQEEEEEEEEEEKKVDLSHSSSTLLPDLMEFERYLNFYKQTMDLLTMNSIISAHEVTLP IVSAAISHLWQTLSEEKKARLLQVWEQQHSAFADLTEACLELAGVEGSMKDSGVDSQGAS CSLESTPEEILFEDAFDVASFLDKSEAQHMSNISAMFATCNSENPEEKFQLYIQIIEFFK SLGCVNTPLNQEPEPPDDDDAMLLKCLETFDDL
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Meiosis-inducer required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. During spermatogenesis, next to its role in meiotic initiation, promotes (but is not required for) spermatogonial differentiation. Can associate with DNA (possibly in an indirect manner), and in vitro can activate DNA transcription.
Gene References into Functions
  1. This study concluded that retinoic acid and STRA8 are conserved factors that control the initiation of meiosis amongst mammals but the role of CYP26B1 as a meiosis-inhibiting factor may be specific to rodents. PMID: 29044428
  2. Combined deletion of Mettl3 and Mettl14 in advanced germ cells with Stra8-GFPCre disrupts spermiogenesis, whereas mice with single deletion of either Mettl3 or Mettl14 in advanced germ cells show normal spermatogenesis. The spermatids from double-mutant mice exhibit impaired translation of haploid-specific genes that are essential for spermiogenesis PMID: 28914256
  3. SMAD4 and STRA8 are essential factors that regulate the female fate of germ cells. PMID: 27606421
  4. SOHLH1 and SOHLH2 were able to directly and cooperatively repress STRA8 expression in cell lines in vitro through binding to its promoter. PMID: 25603532
  5. Our findings strengthen the importance of RA and Dazl in the meiotic transition, provide important details about the Stra8 pathway, and open avenues to investigate early meiosis through analysis of Rec8 induction and function PMID: 25102060
  6. periodic RA-STRA8 signaling intersects with periodic germ-cell competencies to regulate two distinct, cell-type-specific responses: spermatogonial differentiation and meiotic initiation PMID: 25902548
  7. Our findings suggest that the mTOR signaling pathway may have role in the proliferation and stimulation of meiotic initiation of spermatogonial stem cells PMID: 25241363
  8. CYP26B1 suppresses two distinct genetic programs induced by retinoic acid: a Stra8-dependent meiotic pathway, and a Stra8-independent mitotic pathway. PMID: 24576537
  9. cultured XX and XY germ cells at 11.5 and 12.5 days postcoitus (dpc) with an RA receptor inhibitor. Expression of Stra8, a meiosis initiation gene, was suppressed in all groups PMID: 22262692
  10. Stra8-deficient mice had an 88% decrease in teratoma incidence, providing direct evidence that premature initiation of the meiotic program contributes to tumorigenesis. PMID: 22438569
  11. DMRT1 controls Stra8 sex-specifically, activating it in the fetal ovary and repressing it in the adult testis. PMID: 21621532
  12. Stra8 and Dazl are highly expressed in premeiotic germ cells that do not undergo further differentiation in aged mouse ovaries. PMID: 20157580
  13. In this study, a detailed analysis of the EGFP expression pattern in the testis was carried out, showing that the transgene was expressed in meiotic and postmeiotic germ cells and not in undifferentiated germ cells. PMID: 19378319
  14. Endogenous Stra8 gene expression is higher in differentiating spermatogonia than in undifferentiated spermatogonia and could mediate the RA effects on spermatogonial maturation. PMID: 18032419
  15. STRA8 protein expression in adult spermatogonia was induced by RA stimulation, suggesting its role in spermatogonial differentiation. PMID: 18322276
  16. In both the male and female germ lines, meiosis is initiated through retinoic acid induction of Stra8. PMID: 18799751
  17. STRA8 is involved in the process that leads to stable commitment to the meiotic cell cycle. PMID: 18799790
  18. STRA8 associates with DNA and possesses transcriptional activity. PMID: 19805549

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Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Expressed exclusively in premeiotic germ cells in both sexes. In females, is expressed in the embryonic ovary. In males, is expressed in pubertal and adult testes, in premeiotic spermatogenic cells. Expressed by some type A and B spermatogonia, preleptote
Database Links

KEGG: mmu:20899

STRING: 10090.ENSMUSP00000031876

UniGene: Mm.5171

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