Recombinant Mouse Syntaxin-3 (Stx3), partial

Code CSB-EP727366MO
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Source E.coli
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Code CSB-EP727366MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP727366MO
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Source Baculovirus
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Code CSB-MP727366MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Stx3
Uniprot No.
Alternative Names
Stx3; Stx3a; Syntaxin-3
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Potentially involved in docking of synaptic vesicles at presynaptic active zones.
Gene References into Functions
  1. Rab11a-knockdown cells displayed mislocalization of STX3 and the presentation of microvilli in the basolateral compartment PMID: 25673875
  2. Phosphorylation of syntaxin 3B by CaMKII can modulate the assembly of the SNARE complex in ribbon synapses of the retina. PMID: 24680688
  3. The SNARE machinery composed of VAMP7 on Tyrp1-containing vesicles and syntaxin-3 and SNAP23 on melanosomes regulates Tyrp1 trafficking to the melanosome in melanocytes. PMID: 23549422
  4. In insulin secretion, SYN-3 plays a role in the mediation of newcomer SG exocytosis and SG-SG fusion that contributes to biphasic GSIS. PMID: 23132338
  5. Results show that cells in the mouse retina express only syntaxin 3B, while nonneuronal tissues, such as kidney, express only syntaxin 3A. PMID: 18683220

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Subcellular Location
Membrane; Single-pass type IV membrane protein.; [Isoform 3B]: Photoreceptor inner segment. Cell projection, cilium, photoreceptor outer segment.
Protein Families
Syntaxin family
Tissue Specificity
Expressed in the retina (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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