Recombinant Mouse TNF receptor-associated factor 6(Traf6)

Code CSB-YP024154MO
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Source Yeast
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Code CSB-EP024154MO
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Source E.coli
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Code CSB-EP024154MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP024154MO
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Source Baculovirus
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Code CSB-MP024154MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Traf6
Uniprot No. P70196
Alternative Names Traf6; TNF receptor-associated factor 6; EC 2.3.2.27; E3 ubiquitin-protein ligase TRAF6; RING-type E3 ubiquitin transferase TRAF6
Species Mus musculus (Mouse)
Expression Region 1-530
Target Protein Sequence MSLLNCENSC GSSQSSSDCC AAMAASCSAA VKDDSVSGSA STGNLSSSFM EEIQGYDVEF DPPLESKYEC PICLMALREA VQTPCGHRFC KACIIKSIRD AGHKCPVDNE ILLENQLFPD NFAKREILSL TVKCPNKGCL QKMELRHLED HQVHCEFALV NCPQCQRPFQ KCQVNTHIIE DCPRRQVSCV NCAVSMAYEE KEIHDQSCPL ANIICEYCGT ILIREQMPNH YDLDCPTAPI PCTFSVFGCH EKMQRNHLAR HLQENTQLHM RLLAQAVHNV NLALRPCDAA SPSRGCRPED PNYEETIKQL ESRLVRQDHQ IRELTAKMET QSMYVGELKR TIRTLEDKVA EMEAQQCNGI YIWKIGNFGM HLKSQEEERP VVIHSPGFYT GRPGYKLCMR LHLQLPTAQR CANYISLFVH TMQGEYDSHL PWPFQGTIRL TILDQSEALI RQNHEEVMDA KPELLAFQRP TIPRNPKGFG YVTFMHLEAL RQGTFIKDDT LLVRCEVSTR FDMGGLRKEG FQPRSTDAGV
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function E3 ubiquitin ligase that, together with UBE2N and UBE2V1, mediates the synthesis of 'Lys-63'-linked-polyubiquitin chains conjugated to proteins, such as IKBKG, IRAK1, AKT1 and AKT2. Also mediates ubiquitination of free/unanchored polyubiquitin chain that leads to MAP3K7 activation (By similarity). Leads to the activation of NF-kappa-B and JUN. May be essential for the formation of functional osteoclasts. Seems to also play a role in dendritic cells (DCs) maturation and/or activation. Represses c-Myb-mediated transactivation, in B-lymphocytes. Adapter protein that seems to play a role in signal transduction initiated via TNF receptor, IL-1 receptor and IL-17 receptor. Regulates osteoclast differentiation by mediating the activation of adapter protein complex 1 (AP-1) and NF-kappa-B, in response to RANK-L stimulation. Together with MAP3K8, mediates CD40 signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production.
Gene References into Functions
  1. Traf6 plays a crucial role in regulating M1 osteoclast and M2 foreign body giant cell polarization. PMID: 29273889
  2. Authors report that the deletion of TRAF6 in hematopoietic lineage cells resulted in the upregulation of HSPCs in the fetal liver at the prenatal period. However, in the early postnatal period, deletion of TRAF6 drastically diminished HSPCs in the bone marrow (BM), with severe defects in BM development and extramedullary hematopoiesis in the spleen being identified. PMID: 30037215
  3. Study demonstrated that TRAF6 plays an important role in the process of aseptic inflammatory loosening caused by periprosthetic debris, and Low intensity pulsed ultrasound was found to inhibit such inflammation through increasing the level of FBXL2, and further increasing ubiquitination of TRAF6. PMID: 28378753
  4. these findings identify IPMK as a key determinant of TRAF6 stability and elucidate the physiological function of IPMK in TLR-induced innate immunity. PMID: 28439546
  5. Results demonstrate that TRAF6 interacts with MyD88 and suggest that TRAF6 participates in protection from basal autophagy. PMID: 28432355
  6. Klf4 Alleviates Lipopolysaccharide-Induced Inflammation by Inducing Expression of MCP-1 Induced Protein 1 to Deubiquitinate TRAF6. PMID: 29975947
  7. TRAF6 RING dimer employs a concerted allosteric mechanism using both subunits of the TRAF6 dimer to promote ubiquitin (Ub) transfer. PMID: 29432170
  8. Low TRAF6 expression is associated with posterior longitudinal ligament ossification. PMID: 29782494
  9. miR-146a-mediated control of Traf6 expression Is required for the attenuation of proinflammatory cytokine responses, control of T cell tolerance, and prevention of autoimmunity. PMID: 28784800
  10. Data indicate TNF receptor associated factor 6 (TRAF6) as an essential molecular switch leading to cardiac hypertrophy in a transforming growth factor beta-activated kinase 1 (TAK1), -dependent manner. PMID: 27249171
  11. Berberine hydrochloride targets TRAF6 and NFATc1. PMID: 28849049
  12. protein-protein interaction studies on TRAF6 and BSG to get molecular level insights of the reactions. PMID: 28612997
  13. The authors found that keratin 8 suppressed toll-like receptor-induced nuclear factor (NF)-kappaB activation and interacted with the adaptor tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) to prevent its polyubiquitination. PMID: 27586056
  14. MiR-146a/ MiR-146b could down-regulate the TRAF6 and IRAK1 expression and promote proliferation, migration and angiogenesis ability of endothelial progenitor cells, which could be important for recovery of patients with hyperacute ischemic stroke. PMID: 28811005
  15. TRAF6 coiled-coil oligomerization domain primes its interaction with Ubc13/Ub~Ubc13 to confer processivity. Long polyUb chain assembly requires coiled-coil domain of TRAF6 PMID: 28993672
  16. TRAF6 is a key promoter of ischemic signaling cascades and neuronal death after cerebral ischemia/reperfusion injury. TRAF6 upregulation binds and ubiquitinates Rac1 directly, which promotes neuron death through neuroinflammation and neuro-oxidative signals. PMID: 29114077
  17. Data provide a novel association between WDFY3 and the RANKL-induced osteoclastogenesis pathway via the modulation of TRAF6. PMID: 27330028
  18. this study shows that TRAF6 is necessary for the nontranscriptional priming of NLRP3 inflammasome by TLR/IL-1R derived signals PMID: 28739881
  19. TRAF6 prevents the mitochondrial translocation of p53 and spontaneous apoptosis by promoting lysine63-linked ubiquitination of p53 in cytosol. PMID: 27818144
  20. this study shows that an interaction of TRAF6 with cullin-5 promotes TRAF6 polyubiquitination and lipopolysaccharide signaling PMID: 27233966
  21. Consistent with cellular studies, icaritin downregulated TRAF6 and NFATc1 protein expression in CD11b(+) /Gr-1(-/low) osteoclast precursors PMID: 27813153
  22. the SH3 domain of NOSTRIN is involved in the NOSTRIN-TRAF6 interaction and is required for NOSTRIN-induced down-regulation of endothelial cell proteins PMID: 28235804
  23. this study shows that TRAF6 overexpression in hematopoietic stem/progenitor cells results in impaired hematopoiesis and bone marrow failure PMID: 28024152
  24. TRAF6 and H2AX overexpression and gammaH2AX-mediated HIF1alpha enrichment in the nucleus of cancer cells lead to overactivation of HIF1alpha-driven tumorigenesis, glycolysis and metastasis. PMID: 27918549
  25. By studying the response of Traf6(+/+) and Traf6(-/-)mouse embryonic fibroblasts to S. typhimurium infection, this study showed that TRAF6 promoted apoptosis by increasing reactive oxygen species accumulation, which led to increased Bax/Bcl-2 ratio, Bax recruitment to mitochondrial membrane, and release of Cyt c into the cytoplasm. PMID: 27473656
  26. BAT3 negatively regulates lipopolysaccharide-induced NF-kappaB signaling through TRAF6. PMID: 27501752
  27. these data identify Trim13 as a positive regulator of NF-kappaB activation and suggest that lysine 29-linked polyubiquitination is a specific ubiquitin-linked pattern involved in the control of TLR2 signaling. PMID: 28087809
  28. this study shows that the absence TRAF6 expression by dendritic cells leads to spontaneous generation of Th2-associated immune responses and increased susceptibility to model antigen-induced asthma PMID: 28338920
  29. MiR-144 reduces the antiviral response by attenuating the TRAF6-IRF7 pathway to alter the cellular antiviral transcriptional landscape. PMID: 28380049
  30. Data indicate a role of microRNA miR-146a in the modulation of host defense against mycobacterial infection by repressing nitric oxide (NO) production via targeting tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6), which may provide a promising therapeutic target for tuberculosis. PMID: 27025258
  31. TRAF6 was required for the activation of MAPKs ERK1/2 and JNK1/2 PMID: 26619121
  32. Results suggest that microRNA-146a-5p attenuates neuropathic pain partly through inhibition of tumor necrosis factor receptor associated factor-6 and its downstream c-jun N-terminal kinase/CCL2 signaling. PMID: 25957028
  33. we demonstrate that CpG treatment leads to ubiquitin-mediated degradation of DOK3 via interaction with an E3 ligase TNFR-associated factor 6 (TRAF6). PMID: 26548852
  34. TRAF6 is determined to be a direct E3 ligase for GSK3beta, and TRAF6-mediated GSK3beta ubiquitination is essential for poly I:C-dependent cytokine production by promoting the TLR3 adaptor protein TRIF-assembled signalling complex. PMID: 25828701
  35. TIFAB loss increases TRAF6 protein and the dynamic range of TLR4 signaling PMID: 26458771
  36. The MYSM1 SWIRM domain interacts with TRAF6 to terminate PRR pathways for pro-inflammatory and type I interferon responses. PMID: 26474655
  37. Transmembrane motif T6BM2-mediated TRAF6 binding is required for MAVS-related antiviral response. PMID: 26385923
  38. Tax interacted with and activated TRAF6, and triggered its mitochondrial localization, where it conjugated four carboxyl-terminal lysine residues of MCL-1 with lysine 63-linked polyubiquitin chains PMID: 25340740
  39. miR-146a ameliorates liver ischemia/reperfusion injury in vivo and hypoxia/reoxygenation injury in vitro by directly suppressing IRAK1 and TRAF6. PMID: 24987958
  40. NOD2 downregulates colonic inflammation by IRF4-mediated inhibition of K63-linked polyubiquitination of RICK and TRAF6. PMID: 24670424
  41. miR-146a attenuates sepsis-induced cardiac dysfunction by preventing NF-kappaB activation, inflammatory cell infiltration, and inflammatory cytokine production via targeting of IRAK and TRAF6 in both cardiomyocytes and inflammatory monocytic cells. PMID: 26048146
  42. The results suggest that TRAF6, upregulated in spinal cord astrocytes in the late phase after nerve injury, maintains neuropathic pain by integrating TNF-alpha and IL-1beta signaling and activating the JNK/CCL2 pathway in astrocytes. PMID: 25267210
  43. TRAF6 is also required for GM-CSF-induced ubiquitination and activation of Akt. PMID: 25700345
  44. Findings reveal a mechanism by which TRAF6 is regulated and highlight a role for MST4 in limiting inflammatory responses. PMID: 25642822
  45. TRAF6 may play a pivotal role in mediating LPS-induced cardiac injury via Akt2 ubiquitination. PMID: 24805195
  46. miR-125 protects the myocardium from I/R injury by preventing p53-mediated apoptotic signalling and suppressing TRAF6-mediated NF-kappaB activation. PMID: 24576954
  47. TNF receptor-associated factor 6 (TRAF6), a verified target of miR-146a, was up-regulated in miR-146a(-/-) T cells following alloantigen stimulation. PMID: 25205119
  48. important for EBV-encoded LMP1-mediated autoantibody production, as well as germinal center formation PMID: 24170780
  49. Study suggests that while the inhibition of TRAF6 improves muscle structure and function in young mdx mice, its continued inhibition causes more severe myopathy at later stages of disease progression potentially through repressing autophagy. PMID: 24163132
  50. CHIP inhibits NF-kappaB signaling by promoting TRAF6 degradation and plays an important role in osteoclastogenesis and bone remodeling. PMID: 24578159
  51. TRAF6 in regulatory T cells (Tregs) plays important roles in the maintenance of Foxp3 in Tregs. PMID: 24058613
  52. OXO can potentially inhibit several biomarkers related to inflammation through inhibition of ROS-mediated activation of TRAF6-ASK1-p38 pathway. PMID: 23914844
  53. A52 dimer causes TRAF6 self-association, leading to TAK1 recruitment and p38 activation. PMID: 24114841
  54. TRAF6-deficiency leads to a reduction in presenilin protein levels and reduced PS1 ubiquitination. PMID: 23707529
  55. Resveratrol, by suppressing lipopolysaccharide induced TRAF6 expression and ubiquitination, attenuates the lipopolysaccharide induced TLR4-TRAF6, MAP kinase and Akt pathways that can be significant in its anti-inflammatory effects. PMID: 22925919
  56. Our results establish that the IRAK2-TRAF6 interaction is rate limiting for the late, but not the early phase of cytokine production in bone marrow-derived macrophages and plasmacytoid dendritic cells PMID: 23918981
  57. TRAF6 plays a key role in TLR-mediated IRE1alpha activation by catalysing IRE1alpha ubiquitination and blocking the recruitment of protein phosphatase 2A. PMID: 23942232
  58. Itch interacts with the deubiquitinating enzyme and is required for deubiquitination of TRAF6, thus limiting RANKL-induced osteoclast formation PMID: 23782702
  59. role in directing dendritic cell-mediated intestinal immune tolerance PMID: 23791643
  60. TRAF6 and TAK1 play essential roles in the C-type lectin receptor signaling in response to Candida albicans infection. PMID: 23148225
  61. Mycoepoxydiene inhibits lipopolysaccharide-induced inflammatory responses through the suppression of TRAF6 polyubiquitination PMID: 22984582
  62. Nodakenin was found to significantly inhibit the LPS-induced binding of transforming growth factor-beta-activated kinase 1 to tumor necrosis factor receptor-associated factor 6 (TRAF6) by reducing TRAF6 ubiquitination. PMID: 22637723
  63. TRAF3 negatively regulated TRAF6-dependent, CD40-mediated TAK1 activation by limiting TRAF6 recruitment PMID: 22860133
  64. Skeletal muscle-specific depletion of TRAF6 in mice (TRAF6(mko)) improved regeneration of myofibers upon injury with a concomitant increase in the number of satellite cells and activation of the Notch signaling pathway. PMID: 23028045
  65. Toll-like receptor-mediated TRAF6 signaling acts in endothelial cells to promote atherosclerosis but displays atheroprotective, anti-inflammatory and prosurvival functions in myeloid cells. PMID: 22935568
  66. TRAF6-dependent signaling may be a central pathway in osteoclast differentiation, and that TNF superfamily molecules other than RANKL may modify RANK signaling by interaction with TRAF6-associated signaling PMID: 22719861
  67. The findings define a new role for the IKK-related kinases in suppressing IL-17-mediated NF-kappaB activation through TRAF6-dependent Act1 phosphorylation. PMID: 22851696
  68. Results indicate that the anti-inflammatory properties of DCO-6 was through inhibition of reactive oxygen species-dependent activation of TRAF6-ASK1-p38 pathway. PMID: 22720096
  69. NLRC3 inhibited Toll-like receptor (TLR)-dependent activation of the transcription factor NF-kappaB by interacting with the TLR signaling adaptor TRAF6 PMID: 22863753
  70. Results establish proteasome degradation of TRAF6 as a key molecular target for the anti-inflammatory pathway triggered by lipoxins and L-kynurenine, critical counter-regulatory mediators in the innate and adaptive immune systems. PMID: 22693634
  71. Kaposi's sarcoma associated herpesvirus encoded viral FLICE inhibitory protein K13 activates NF-kappaB pathway independent of TRAF6, TAK1 and LUBAC PMID: 22590573
  72. TRAF6 mediates IL-1beta/LPS-induced suppression of TGF-beta signaling through its interaction with the type III TGF-beta receptor. PMID: 22427868
  73. Traf6 mediates starvation-induced muscle atrophy in mice. PMID: 22290431
  74. Our findings defined a novel function for TRIM38 to prevent excessive TLR-induced inflammatory responses through proteasomal degradation of TRAF6. PMID: 22323536
  75. Tumor necrosis factor receptor-associated factor 6 (TRAF6) associates with huntingtin protein and promotes its atypical ubiquitination to enhance aggregate formation. PMID: 21454471
  76. study identifies a previously unrecognized role for NLRX1 in the negative regulation of TLR-induced NF-kappaB activation by dynamically interacting with TRAF6 and the IKK complex PMID: 21703539
  77. TRAF6 regulates proliferation and differentiation of skeletal myoblasts PMID: 21131124
  78. TRAF6 negatively regulates IL-2 signaling by binding to IL-2Rb to inhibit Jak1 activation. PMID: 21155952
  79. Muscle-specific depletion of TRAF6 inhibits skeletal muscle wasting in response to denervation and in a mouse model of cancer cachexia. PMID: 21187332
  80. Data suggest that pro- and anti-inflammatory features of TRAF6 signaling outweigh each other in the context of atherosclerosis. PMID: 20644648
  81. miR-146a - knockdown results in features of human 5q- syndrome phenotype with target of tumor necrosis factor receptor-associated facter-6. PMID: 19898489
  82. Blockage of common cytokine receptor beta subunit-induced NF-kappaB activation in TRAF6 knockout cells can be rescued by expression of wild-type TRAF6. PMID: 20622119
  83. TRAF6 inhibits Th17 differentiation and TGF-beta-mediated suppression of IL-2. PMID: 20351308
  84. Data show that knockdown of TNFR-associated factor 6 or NF-kappaB activator 1 in 70Z/3 pre-B cells led to decreased Rgs16 expression, indicating that both of these two genes are involved in IL-17-mediated activation of NF-kappaB signaling in B cells. PMID: 20139273
  85. The CD40-TRAF6 axis controls affinity maturation and the generation of long-lived plasma cells. PMID: 11967542
  86. TRAF6, associated with the c-Src complex, modulates the IL-1-induced cytoskeletal reorganization signal essential for activation of osteoclast-like cells in vitro. PMID: 11994463
  87. deficiency results in hypohidrotic ectodermal dysplasia PMID: 12060722
  88. crystal structures of TRAF6, alone and in complex with TRAF6-binding peptides from CD40 and TRANCE-R, members of the TNFR superfamily, to gain insight into the mechanism by which TRAF6 mediates several signalling cascades PMID: 12140561
  89. the interaction of RANK with TRAF6 is absolutely required for the proper formation of cytoskeletal structures and functional resorptive activity of osteoclasts PMID: 12185073
  90. TIFA is likely to mediate IRAK-1/TRAF6 interaction upon IL-1 stimulation. PMID: 12566447
  91. Results demonstrate that c-Src and TRAF6 are key mediators of interleukin-1-induced AP-1 activation and provide evidence of cross talk between c-Src and TRAF6 molecules through PI3 kinase-Akt-JNK pathways. PMID: 12631284
  92. TRAF6 translocation depends on raft membrane microdomains PMID: 12637570
  93. TRAF6 is a diverging point in the Toll-like receptor 9-signaling pathway for CpG DNA-mediated MAPK activation PMID: 12902324
  94. TRAF6 plays an essential role in regulating self-tolerance and immune homeostasis: its deletion induces a dominant Th2-type polarized autoimmune response. PMID: 14634083
  95. TRAF-6 has a role in RANKL inhibition of cell growth and induction of apoptosis PMID: 14645259
  96. IRAK1 and TRAF6 are essential for LMP1-induced NF-kappaB activation. PMID: 14673102
  97. in additional to ectodysplasin, another TNF pathway involving Troy/Traf6 is involved in molar tooth cusp formation PMID: 14699584
  98. TRAF6 plays a critical role mediating developmental NF-kB activation in many but not all tissues. PMID: 15305292
  99. MAST205 functions as a scaffolding protein controlling TRAF6 activity and, therefore, plays an important role in regulating inflammatory responses. PMID: 15308666
  100. TLR-mediated IFN-alpha induction requires the formation of a complex consisting of MyD88, TRAF6 and IRF7 as well as TRAF6-dependent ubiquitination. PMID: 15361868

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Subcellular Location Cytoplasm, Cytoplasm, cell cortex, Nucleus, Lipid droplet
Protein Families TNF receptor-associated factor family, A subfamily
Tissue Specificity Highly expressed in brain, lung, liver, skeletal muscle, and kidney; lower expression in heart, spleen, and testis.
Database Links

KEGG: mmu:22034

STRING: 10090.ENSMUSP00000004949

UniGene: Mm.292729

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