Recombinant Mouse Ubiquitin-like protein ATG12 (Atg12)

Code CSB-YP861455MO
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Source Yeast
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Code CSB-EP861455MO
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Source E.coli
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Code CSB-EP861455MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861455MO
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Source Baculovirus
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Code CSB-MP861455MO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Atg12
Uniprot No.
Alternative Names
Atg12; Apg12; Apg12lUbiquitin-like protein ATG12; Autophagy-related protein 12; APG12-like
Species
Mus musculus (Mouse)
Expression Region
1-141
Target Protein Sequence
MSEDSEVVLQ LPSAPVGAGG ESLPELSPET ATPEPPSSAA VSPGTEEPPG DTKKKIDILL KAVGDTPIMK TKKWAVERTR TIQGLIDFIK KFLKLVASEQ LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW G
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.; (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection.
Gene References into Functions
  1. miR-23a downregulation modulates the inflammatory response by targeting ATG12-mediated autophagy. PMID: 29845275
  2. Axonal development is compromised in brains of mir505 knockout mice, in which autophagy signaling and formation of autophagosomes are consistently enhanced. miR505 acts via targeting Atg12. PMID: 28820282
  3. miR-30b inhibited autophagy to alleviate hepatic ischemia-reperfusion injury by decreasing the Atg12-Atg5 conjugate. PMID: 27182160
  4. Although genetic deletion of either Atg12 or Atg5 renders POMC neurons autophagy-deficient, mice lacking Atg5 in POMC neurons do not exhibit these phenotypes. PMID: 25585051
  5. ATG12-ATG3 interacts with Alix to promote basal autophagic flux and late endosome function. PMID: 25686249
  6. Atg5-Atg12/Atg16L1 protein complex is required for IFNgamma-mediated host defense against murine norovirus infection. PMID: 22520467
  7. Vaccinia virus actively disrupts the cellular autophagy through a novel molecular mechanism that is associated with aberrant LC3 lipidation and a direct conjugation between ATG12 and ATG3. PMID: 22024753
  8. Despite forming the Atg12-Atg5-Atg16L2 complex, Atg16L2 is not recruited to phagophores and is mostly present in the cytosol. PMID: 22082872
  9. Autophagy participated the process of immune response of anti-MTB. Atg12 were the important molecule which control the formation of autophagy when MTB infected. PMID: 19174008
  10. Overexpression of Atg5 or Atg12 resulted in Atg5-Atg12 conjugate formation and suppression of RNA-mediated signaling. PMID: 17709747

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Subcellular Location
Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein.
Protein Families
ATG12 family
Tissue Specificity
Ubiquitous.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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